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Article

Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells

  • Authors:
    • Hong-Liang Li
    • Shu Zhang
    • Yuan Wang
    • Rong-Rui Liang
    • Jun Li
    • Peng An
    • Zi-Ming Wang
    • Jun Yang
    • Zong-Fang Li
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, P.R. China, Engineering Research Center of Biotherapy and Translational Medicine of Shaanxi Province, Xi'an Jiaotong University, Xi'an 710004, P.R. China, Department of Pathology, Shaanxi Provincial People's Hospital, Xi'an 710068, P.R. China
  • Pages: 266-270
    |
    Published online on: October 9, 2012
       https://doi.org/10.3892/mmr.2012.1123
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Abstract

Recurrence of bladder cancer following transurethral resection of bladder tumor (TURBt) is an obstacle in clinical management. In the current study, we investigated the antitumor activity of baicalein, a Chinese herbal medicine, against T24 bladder cancer cells in vitro. Baicalein inhibited growth and caused G1/S arrest of the cell cycle in the T24 cells. Moreover, baicalein induced apoptosis via loss of mitochondrial transmembrane potential (ΔΨm), release of cytochrome c and activation of caspase-9 and caspase-3. Baicalein inhibited Akt phosphorylation, downregulated Bcl-2 expression and upregulated Bax expression, which in turn increased the ratio of Bax/Bcl-2. Our results demonstrate that baicalein repressed growth inhibition and induced apoptosis via loss of ΔΨm and activation of caspase-9 and caspase-3 in T24 bladder cancer cells, which indicates that baicalein may be an effective agent in the clinical management of bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li H, Zhang S, Wang Y, Liang R, Li J, An P, Wang Z, Yang J and Li Z: Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells. Mol Med Rep 7: 266-270, 2013.
APA
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P. ... Li, Z. (2013). Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells. Molecular Medicine Reports, 7, 266-270. https://doi.org/10.3892/mmr.2012.1123
MLA
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P., Wang, Z., Yang, J., Li, Z."Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells". Molecular Medicine Reports 7.1 (2013): 266-270.
Chicago
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P., Wang, Z., Yang, J., Li, Z."Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells". Molecular Medicine Reports 7, no. 1 (2013): 266-270. https://doi.org/10.3892/mmr.2012.1123
Copy and paste a formatted citation
x
Spandidos Publications style
Li H, Zhang S, Wang Y, Liang R, Li J, An P, Wang Z, Yang J and Li Z: Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells. Mol Med Rep 7: 266-270, 2013.
APA
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P. ... Li, Z. (2013). Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells. Molecular Medicine Reports, 7, 266-270. https://doi.org/10.3892/mmr.2012.1123
MLA
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P., Wang, Z., Yang, J., Li, Z."Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells". Molecular Medicine Reports 7.1 (2013): 266-270.
Chicago
Li, H., Zhang, S., Wang, Y., Liang, R., Li, J., An, P., Wang, Z., Yang, J., Li, Z."Baicalein induces apoptosis via a mitochondrial-dependent caspase activation pathway in T24 bladder cancer cells". Molecular Medicine Reports 7, no. 1 (2013): 266-270. https://doi.org/10.3892/mmr.2012.1123
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