Effects of apocynin on oxidative stress and expression of apoptosis-related genes in testes of diabetic rats

Li,Mingchao; Liu,Zhuo; Zhuan,Li; Wang,Tao; Guo,Shuiming; Wang,Shaogang; Liu,Jihong; Ye,Zhangqun

doi:10.3892/mmr.2012.1132

January 2013 Volume 7 Issue 1

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January 2013 Volume 7 Issue 1

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Article

Effects of apocynin on oxidative stress and expression of apoptosis-related genes in testes of diabetic rats

Authors:
- Mingchao Li
- Zhuo Liu
- Li Zhuan
- Tao Wang
- Shuiming Guo
- Shaogang Wang
- Jihong Liu
- Zhangqun Ye
View Affiliations / Copyright

Affiliations: Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, P.R. China
Pages: 47-52
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Published online on: October 17, 2012

https://doi.org/10.3892/mmr.2012.1132
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Abstract

Reactive oxygen species (ROS) are important in the development of diabetic testicular dysfunction. Overproduction of ROS promotes the process of apoptosis, which shows that there is a crosstalk between oxidative stress and apoptosis. Recent research has suggested that NADPH oxidase is the main source of ROS. In this study, we investigated whether the NADPH oxidase inhibitor, apocynin, can improve diabetes‑induced testicular dysfunction by suppressing oxidative stress. The streptozocin (STZ)-induced diabetic rats were administered apocynin, and the mRNA and protein expression of Bax, Bcl-2, p47phox and p67phox was examined by real-time PCR (RT-PCR) and western blot analysis. Production of ROS was measured by thiobarbituric acid reactive substances (TBARS) assay. Terminal-deoxynucleoitidyl transferase mediated nick end-labeling (TUNEL) assay was used to detect apoptosis and ELISA was used to detect total testosterone levels. The mRNA and protein expression of Bcl-2 was signiﬁcantly reduced, and that of Bax, p47phox and p67phox was signiﬁcantly increased in the diabetic rats compared to the control group. Apocynin signiﬁcantly increased the expression of Bcl-2 and decreased the expression of Bax, p47phox and p67phox at both the mRNA and protein levels. The production of ROS and apoptotic cells signiﬁcantly increased in the diabetic group compared to the control group. Apocynin signiﬁcantly reduced the production of ROS and apoptotic cells and increased the total testosterone level. In conclusion, apocynin is capable of ameliorating testicular dysfunction.

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1	Aybek H, Aybek Z, Rota S, Sen N and Akbulut M: The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress. Fertil Steril. 90:755–760. 2008. View Article : Google Scholar : PubMed/NCBI
2	Tang XY, Zhang Q, Dai DZ, Ying HJ, Wang QJ and Dai Y: Effects of strontium fructose 1,6-diphosphate on expression of apoptosis-related genes and oxidative stress in testes of diabetic rats. Int J Urol. 15:251–256. 2008. View Article : Google Scholar : PubMed/NCBI
3	Shrilatha B and Muralidhara: Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: its progression and genotoxic consequences. Reprod Toxicol. 23:578–587. 2007. View Article : Google Scholar : PubMed/NCBI
4	Boujbiha MA, Hamden K, Guermazi F, Bouslama A, Omezzine A, Kammoun A and El Feki A: Testicular toxicity in mercuric chloride treated rats: association with oxidative stress. Reprod Toxicol. 28:81–89. 2009. View Article : Google Scholar : PubMed/NCBI
5	Kosova B, Cetintaş VB, Yavaşoğlu A, Altay B and Aktuğ H: From a molecular biological viewpoint, does endothelin type A receptor antagonist therapy reduce diabetes-induced testicular damage in rats? Urology. 77:250.e7–13. 2011. View Article : Google Scholar
6	Sainio-Pöllänen S, Henriksén K, Parvinen M, Simell O and Pöllänen P: Stage-specific degeneration of germ cells in the seminiferous tubules of nonobese diabetic mice. Int J Androl. 20:243–253. 1997.PubMed/NCBI
7	Koh PO: Streptozotocin-induced diabetes increases the interaction of Bad/Bcl-xL and decreases the binding of pBad/14-3-3 in rat testis. Life Sci. 81:1079–1084. 2007. View Article : Google Scholar : PubMed/NCBI
8	Jin L, Lagoda G, Leite R, Webb RC and Burnett AL: NADPH oxidase activation: a mechanism of hypertension-associated erectile dysfunction. J Sex Med. 5:544–551. 2008. View Article : Google Scholar : PubMed/NCBI
9	Jin L and Burnett AL: NADPH oxidase: recent evidence for its role in erectile dysfunction. Asian J Androl. 10:6–13. 2008. View Article : Google Scholar : PubMed/NCBI
10	Cai H, Griendling KK and Harrison DG: The vascular NAD(P)H oxidases as therapeutic targets in cardiovascular diseases. Trends Pharmacol Sci. 24:471–478. 2003. View Article : Google Scholar : PubMed/NCBI
11	Taye A, Saad AH, Kumar AH and Morawietz H: Effect of apocynin on NADPH oxidase-mediated oxidative stress-LOX-1-eNOS pathway in human endothelial cells exposed to high glucose. Eur J Pharmacol. 627:42–48. 2010. View Article : Google Scholar : PubMed/NCBI
12	Cotter MA and Cameron NE: Effect of the NAD(P)H oxidase inhibitor, apocynin, on peripheral nerve perfusion and function in diabetic rats. Life Sci. 73:1813–1824. 2003. View Article : Google Scholar : PubMed/NCBI
13	Olukman M, Orhan CE, Celenk FG and Ulker S: Apocynin restores endothelial dysfunction in streptozotocin diabetic rats through regulation of nitric oxide synthase and NADPH oxidase expressions. J Diabetes Complications. 24:415–423. 2010. View Article : Google Scholar
14	Xu M, Dai DZ, Zhang Q, Cheng YS and Dai Y: Upregulated NADPH oxidase contributes to diabetic testicular complication and is relieved by strontium fructose 1,6-diphosphate. Exp Clin Endocrinol Diabetes. 118:459–465. 2010. View Article : Google Scholar : PubMed/NCBI
15	Zhao Y, Tan Y, Dai J, et al: Exacerbation of diabetes-induced testicular apoptosis by zinc deficiency is most likely associated with oxidative stress, p38 MAPK activation, and p53 activation in mice. Toxicol Lett. 200:100–106. 2011. View Article : Google Scholar : PubMed/NCBI
16	Unlüçerçi Y, Bekpinar S and Koçak H: Testis glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase activities in aminoguanidine-treated diabetic rats. Arch Biochem Biophys. 379:217–220. 2000.PubMed/NCBI
17	Shrilatha B and Muralidhara: Occurrence of oxidative impairments, response of antioxidant defences and associated biochemical perturbations in male reproductive milieu in the Streptozotocin-diabetic rat. Int J Androl. 30:508–518. 2007. View Article : Google Scholar : PubMed/NCBI
18	Koh PO: Streptozotocin-induced diabetes increases apoptosis through JNK phosphorylation and Bax activation in rat testes. J Vet Med Sci. 69:969–971. 2007. View Article : Google Scholar : PubMed/NCBI
19	Kaushal N and Bansal MP: Dietary selenium variation-induced oxidative stress modulates CDC2/cyclin B1 expression and apoptosis of germ cells in mice testis. J Nutr Biochem. 18:553–564. 2007. View Article : Google Scholar : PubMed/NCBI
20	Boekelheide K, Fleming SL, Johnson KJ, Patel SR and Schoenfeld HA: Role of Sertoli cells in injury-associated testicular germ cell apoptosis. Proc Soc Exp Biol Med. 225:105–115. 2000. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of apocynin on oxidative stress and expression of apoptosis-related genes in testes of diabetic rats

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