Proteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: A pilot study

Bhonsle,Hemangi  S.; Korwar,Arvind  M.; Chougale,Ashok  D.; Kote,Sachin  S.; Dhande,Nitin  L.; Shelgikar,Kishore  M.; Kulkarni,Mahesh  J.

doi:10.3892/mmr.2012.1223

February 2013 Volume 7 Issue 2

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February 2013 Volume 7 Issue 2

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Article

Proteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: A pilot study

Authors:
- Hemangi S. Bhonsle
- Arvind M. Korwar
- Ashok D. Chougale
- Sachin S. Kote
- Nitin L. Dhande
- Kishore M. Shelgikar
- Mahesh J. Kulkarni
View Affiliations / Copyright

Affiliations: Proteomics Facility, Division of Biochemical Sciences, Council of Scientific and Industrial Research, National Chemical Laboratory, Pune 411008, India, Maharashtra Medical Research Society, Pune 411008, India
Pages: 495-498
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Published online on: December 4, 2012

https://doi.org/10.3892/mmr.2012.1223
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Abstract

Proteomic approaches aid in gaining a better understanding of the pathophysiology of diabetic complications. In view of this, differential protein expression in diabetic plasma samples was studied by a combination of proteomic and western blot analyses. Diabetic plasma samples were categorized based on glycated haemoglobin levels as controlled diabetes (CD; 7-8%), poorly controlled diabetes (PCD; >8%) and non-diabetic control (ND;<6.4%). Two-dimensional electrophoresis and liquid chromatography‑mass spectrometry revealed differential expression of proteins including upregulation of fibrinogen and haptoglobin and downregulation of vitamin D binding protein, α-1-antitrypsin, transthyretin and apolipoprotein A1 (Apo A1) in diabetic compared with non-diabetic plasma samples. Amongst these proteins, Apo A1 downregulation was prominent in PCD. Downregulation of Apo A1 may serve as an early predictive marker of diabetic complications.

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1	Anderson NL and Anderson NG: The human plasma proteome: history, character and diagnostic prospects. Mol Cell Proteomics. 1:845–867. 2002. View Article : Google Scholar : PubMed/NCBI
2	Anderson NL: The clinical plasma proteome: a survey of clinical assays for proteins in plasma and serum. Clin Chem. 56:177–185. 2010. View Article : Google Scholar : PubMed/NCBI
3	Taylor R and Agius L: The biochemistry of diabetes. Biochem J. 250:625–640. 1988.PubMed/NCBI
4	Chase HP, Jackson WE, Hoops SL, et al: Glucose control and the renal and retinal complications of insulin-dependent diabetes. JAMA. 261:1155–1160. 1989. View Article : Google Scholar : PubMed/NCBI
5	Takahashi S, Uchino H, Shimizu T, et al: Comparison of glycated albumin (GA) and glycated hemoglobin (HbA1c) in type 2 diabetic patients: usefulness of GA for evaluation of short-term changes in glycemic control. Endocr J. 54:139–144. 2007. View Article : Google Scholar : PubMed/NCBI
6	Sundsten T and Ortsater H: Proteomics in diabetes research. Mol Cell Endocrinol. 297:93–103. 2009. View Article : Google Scholar
7	Brownlee M: Biochemistry and molecular cell biology of diabetic complications. Nature. 414:813–820. 2001. View Article : Google Scholar : PubMed/NCBI
8	Giacco F and Brownlee M: Oxidative stress and diabetic complications. Circ Res. 107:1058–1070. 2010. View Article : Google Scholar : PubMed/NCBI
9	Bousova I, Bakala H, Chudacek R, et al: Glycation-induced inactivation of aspartate aminotransferase, effect of uric acid. Mol Cell Biochem. 278:85–92. 2005. View Article : Google Scholar : PubMed/NCBI
10	Hashemi M, Naderi M, Rashidi H, et al: Impaired activity of serum alpha-1-antitrypsin in diabetes mellitus. Diabetes Res Clin Pract. 75:246–248. 2007. View Article : Google Scholar : PubMed/NCBI
11	Mohammad TG, Mojtaba R and Mohsen R: Study of nonenzymatic glycation of transferrin and its effect on iron-binding antioxidant capacity. Iran J Basic Med Sci. 13:194–199. 2010.
12	Jung T and Grune T: The proteasome and its role in the degradation of oxidized proteins. IUBMB Life. 11:743–752. 2008. View Article : Google Scholar : PubMed/NCBI
13	Winter WE and Schatz DA: Autoimmune markers in diabetes. Clin Chem. 57:168–175. 2011. View Article : Google Scholar : PubMed/NCBI
14	Tessari P, Kiwanuka E, Millioni R, et al: Albumin and fibrinogen synthesis and insulin effect in type 2 diabetic patients with normoalbuminuria. Diabetes Care. 29:323–328. 2006. View Article : Google Scholar : PubMed/NCBI
15	Peavy DE, Taylor JM and Jefferson LS: Correlation of albumin production rates and albumin mRNA levels in livers of normal, diabetic and insulin-treated diabetic rats. Proc Natl Acad Sci USA. 75:5879–5883. 1978. View Article : Google Scholar : PubMed/NCBI
16	Blanton D, Han Z, Bierschenk L, et al: Reduced serum vitamin D-binding protein levels are associated with type 1 diabetes. Diabetes. 60:2566–2570. 2011. View Article : Google Scholar : PubMed/NCBI
17	Ceriello A: Fibrinogen and diabetes mellitus: is it time for intervention trials? Diabetologia. 40:731–734. 1997. View Article : Google Scholar : PubMed/NCBI
18	Engstrom G, Hedblad B, Eriksson KF, et al: Complement C3 is a risk factor for the development of diabetes. A population-based cohort study. Diabetes. 54:570–575. 2005. View Article : Google Scholar : PubMed/NCBI
19	Lapolla A, Brioschi M, Banfi C, et al: On the search for glycated lipoprotein ApoA-I in the plasma of diabetic and nephropathic patients. J Mass Spectrom. 43:74–81. 2008. View Article : Google Scholar : PubMed/NCBI
20	Cheng FY, Blackburn K, Lin YM, et al: Absolute protein quantification by LC/MS(E) for global analysis of salicylic acid-induced plant protein secretion responses. J Proteome Res. 8:82–93. 2009. View Article : Google Scholar : PubMed/NCBI
21	Quintao EC, Medina WL and Passarelli M: Reverse cholesterol transport in diabetes mellitus. Diabetes Metab Res Rev. 16:237–250. 2000. View Article : Google Scholar : PubMed/NCBI
22	Vuilleumier N, Bas S, Pagano S, et al: Anti-apolipoprotein A-1 IgG predicts major cardiovascular events in patients with rheumatoid arthritis. Arthritis Rheum. 62:2640–2650. 2010. View Article : Google Scholar : PubMed/NCBI
23	Montecucco F, Vuilleumier N, Pagano S, et al: Anti-Apolipoprotein A-1 auto-antibodies are active mediators of atherosclerotic plaque vulnerability. Eur Heart J. 32:412–421. 2011. View Article : Google Scholar : PubMed/NCBI
24	Haas MJ, Horani M, Mreyoud A, et al: Suppression of apolipoprotein AI gene expression in HepG2 cells by TNF alpha and IL-1beta. Biochim Biophys Acta. 1623:120–128. 2003. View Article : Google Scholar : PubMed/NCBI
25	Mooradian AD, Haas MJ and Wong NC: Transcriptional control of apolipoprotein A-I gene expression in diabetes. Diabetes. 53:513–520. 2004. View Article : Google Scholar : PubMed/NCBI
26	Mallick KA, Joshi MR and Bhat PG: A study on Apo B100/Apo A-I ratio in uncontrolled type 2 diabetes mellitus. Int J Appl Biol Pharm Technol. 2:379–384. 2011.
27	Wagner AM and Ordonez-Llanos J: Apolipoproteins and prediction of fatal myocardial infarction. Lancet. 359:1863–1864. 2002. View Article : Google Scholar : PubMed/NCBI
28	Zoppini G, Targher G, Chonchol M, et al: Higher HDL cholesterol levels are associated with a lower incidence of chronic kidney disease in patients with type 2 diabetes. Nutr Metab Cardiovasc Dis. 19:580–586. 2009. View Article : Google Scholar : PubMed/NCBI