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Article

Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation

  • Authors:
    • Hai-Feng Shi
    • Jie-Fu Yang
    • Qian Wang
    • Ruo-Gu Li
    • Ying-Jia Xu
    • Xin-Kai Qu
    • Wei-Yi Fang
    • Xu Liu
    • Yi-Qing Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Beijing Hospital, Beijing 100730, P.R. China, Department of Cardiology and Cardiovascular Research, Shanghai Chest Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200030, P.R. China
  • Pages: 767-774
    |
    Published online on: December 28, 2012
       https://doi.org/10.3892/mmr.2012.1252
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Abstract

Atrial fibrillation (AF) is the most common form of cardiac arrhythmia observed in clinical practice and a major contributor to cardiovascular morbidity and mortality. Accumulating evidence indicates a substantial genetic basis for AF. However, AF is genetically heterogeneous and the hereditary components responsible for AF remain to be identified in the majority of patients. The cardiac gap junction protein α 5 (GJA5) is specifically expressed in atrial myocytes and is associated with the coordinated electrical activation of the atria, providing a rationale to screen GJA5 as a logical candidate gene for AF. A cohort of 310 unrelated patients with lone AF and their available relatives were included in this study. A group of 200 unrelated healthy individuals matched for age, gender and race were also included as controls. The entire coding region and splice sites of the GJA5 gene were initially sequenced in 310 unrelated AF patients. The relatives of mutation carriers and 200 controls were subsequently genotyped for the presence of identified mutations. As a result, 4 novel heterozygous GJA5 mutations, p.K107R, p.L223M, p.Q236H and p.I257L, were identified in 4 of 310 unrelated AF patients, respectively, with a prevalence of ~1.29%. Genetic analysis of the carriers' families showed that in each family the missense mutation was present in all the affected family members. Absent in the 400 reference alleles, these mutations altered the amino acids highly conserved among various species, with the exception of p.I257L. In conclusion, this study expands the spectrum of GJA5 mutations associated with AF and provides novel insights into the molecular basis of AF, suggesting potential implications for the improved, gene-specific rhythm control strategies.
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Spandidos Publications style
Shi H, Yang J, Wang Q, Li R, Xu Y, Qu X, Fang W, Liu X and Yang Y: Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation. Mol Med Rep 7: 767-774, 2013.
APA
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X. ... Yang, Y. (2013). Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation. Molecular Medicine Reports, 7, 767-774. https://doi.org/10.3892/mmr.2012.1252
MLA
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X., Fang, W., Liu, X., Yang, Y."Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation". Molecular Medicine Reports 7.3 (2013): 767-774.
Chicago
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X., Fang, W., Liu, X., Yang, Y."Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation". Molecular Medicine Reports 7, no. 3 (2013): 767-774. https://doi.org/10.3892/mmr.2012.1252
Copy and paste a formatted citation
x
Spandidos Publications style
Shi H, Yang J, Wang Q, Li R, Xu Y, Qu X, Fang W, Liu X and Yang Y: Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation. Mol Med Rep 7: 767-774, 2013.
APA
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X. ... Yang, Y. (2013). Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation. Molecular Medicine Reports, 7, 767-774. https://doi.org/10.3892/mmr.2012.1252
MLA
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X., Fang, W., Liu, X., Yang, Y."Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation". Molecular Medicine Reports 7.3 (2013): 767-774.
Chicago
Shi, H., Yang, J., Wang, Q., Li, R., Xu, Y., Qu, X., Fang, W., Liu, X., Yang, Y."Prevalence and spectrum of GJA5 mutations associated with lone atrial fibrillation". Molecular Medicine Reports 7, no. 3 (2013): 767-774. https://doi.org/10.3892/mmr.2012.1252
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