Open Access

Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells

  • Authors:
    • Maelinda Daker
    • Saatheeyavaane Bhuvanendran
    • Munirah Ahmad
    • Kenzo Takada
    • Alan Soo‑Beng Khoo
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  • Published online on: December 28, 2012     https://doi.org/10.3892/mmr.2012.1253
  • Pages: 731-741
  • Copyright: © Daker et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Nasopharyngeal carcinoma (NPC) is a unique tumour of epithelial origin with a distinct geographical distribution, closely associated with the Epstein‑Barr virus (EBV). EBV‑encoded RNAs (EBERs) are small non‑polyadenylated RNAs that are abundantly expressed in latent EBV‑infected NPC cells. To study the role of EBERs in NPC, we established stable expression of EBERs in HK1, an EBV‑negative NPC cell line. Cells expressing EBERs consistently exhibited an increased growth rate. However, EBERs did not confer resistance towards cisplatin‑induced apoptosis or promote migration or invasion ability in the cells tested. Using microarray gene expression profiling, we identified potential candidate genes that were deregulated in NPC cells expressing EBERs. Gene Ontology analysis of the data set revealed that EBERs upregulate the cellular lipid metabolic process. Upregulation of low‑density lipoprotein receptor (LDLR) and fatty acid synthase (FASN) was observed in EBER‑expressing cells. NPC cells exhibited LDL‑dependent cell proliferation. In addition, a polyphenolic flavonoid compound, quercetin, known to inhibit FASN, was found to inhibit proliferation of NPC cells.
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March 2013
Volume 7 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Daker M, Bhuvanendran S, Ahmad M, Takada K and Khoo AS: Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells. Mol Med Rep 7: 731-741, 2013
APA
Daker, M., Bhuvanendran, S., Ahmad, M., Takada, K., & Khoo, A.S. (2013). Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells. Molecular Medicine Reports, 7, 731-741. https://doi.org/10.3892/mmr.2012.1253
MLA
Daker, M., Bhuvanendran, S., Ahmad, M., Takada, K., Khoo, A. S."Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells". Molecular Medicine Reports 7.3 (2013): 731-741.
Chicago
Daker, M., Bhuvanendran, S., Ahmad, M., Takada, K., Khoo, A. S."Deregulation of lipid metabolism pathway genes in nasopharyngeal carcinoma cells". Molecular Medicine Reports 7, no. 3 (2013): 731-741. https://doi.org/10.3892/mmr.2012.1253