Changes in T lymphocyte subsets and intracellular cytokines after transfer of chemically extracted acellular nerve allografts

Li,Wei; Wu,Wen-Wen; Lin,Xing-Shi; Hou,Shu-Xun; Zhong,Hong-Bin; Ruan,Di-Ke

doi:10.3892/mmr.2012.747

April 2012 Volume 5 Issue 4

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April 2012 Volume 5 Issue 4

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Article

Changes in T lymphocyte subsets and intracellular cytokines after transfer of chemically extracted acellular nerve allografts

Authors:
- Wei Li
- Wen-Wen Wu
- Xing-Shi Lin
- Shu-Xun Hou
- Hong-Bin Zhong
- Di-Ke Ruan
View Affiliations / Copyright

Affiliations: Department of Orthopaedics, Navy General Hospital, Beijing 100037, P.R. China, Department of Orthopaedics, PLA General Hospital, Beijing 100853, P.R. China, Department of Immunity, PLA General Hospital, Beijing 100853, P.R. China
Pages: 1080-1086
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Published online on: January 9, 2012

https://doi.org/10.3892/mmr.2012.747
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Abstract

The aim of the present study was to observe the immune mechanism underlying the rejection of chemically extracted acellular nerve allografts for use in clinical applications. A total of 128 BALB/c mice were randomly divided into a negative contrast group (NC, 32 mice), a fresh autograft group (AG, 32 mice), a fresh allogeneic nerve group (FN, 32 mice) and a chemically extracted acellular allogeneic nerve group (CEN, 32 mice). Various types of nerve grafts were implanted into the thigh muscle of BALB/C mice in the corresponding groups. At 3, 7, 14 and 28 days post-operation, the mice (8 cases from each group) were sacrificed and their spleens were extracted. The spleens were ground into paste. The erythrocytes and other cells were lysed using distilled water and the T lymphocytes were collected. Monoclonal antibodies (CD3, CD4, CD8, CD25, IL-2, IFN-γ and TNF-α) were then added to the solution. The Facial Action Coding System was used to determine the positive rates of the cells combined with the monoclonal antibodies above. No significant statistical differences were observed between the CEN, NC and AG groups. However, some data of the FN group were significantly higher than those of the other groups at the corresponding time. No obvious immune rejections were observed among the chemically extracted acellular nerve allografts compared with fresh nerve autograft.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Changes in T lymphocyte subsets and intracellular cytokines after transfer of chemically extracted acellular nerve allografts

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