Changes in T lymphocyte subsets and intracellular cytokines after transfer of chemically extracted acellular nerve allografts
- Authors:
- Wei Li
- Wen-Wen Wu
- Xing-Shi Lin
- Shu-Xun Hou
- Hong-Bin Zhong
- Di-Ke Ruan
View Affiliations
Affiliations: Department of Orthopaedics, Navy General Hospital, Beijing 100037, P.R. China, Department of Orthopaedics, PLA General Hospital, Beijing 100853, P.R. China, Department of Immunity, PLA General Hospital, Beijing 100853, P.R. China
- Published online on: January 9, 2012 https://doi.org/10.3892/mmr.2012.747
-
Pages:
1080-1086
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
The aim of the present study was to observe the immune mechanism underlying the rejection of chemically extracted acellular nerve allografts for use in clinical applications. A total of 128 BALB/c mice were randomly divided into a negative contrast group (NC, 32 mice), a fresh autograft group (AG, 32 mice), a fresh allogeneic nerve group (FN, 32 mice) and a chemically extracted acellular allogeneic nerve group (CEN, 32 mice). Various types of nerve grafts were implanted into the thigh muscle of BALB/C mice in the corresponding groups. At 3, 7, 14 and 28 days post-operation, the mice (8 cases from each group) were sacrificed and their spleens were extracted. The spleens were ground into paste. The erythrocytes and other cells were lysed using distilled water and the T lymphocytes were collected. Monoclonal antibodies (CD3, CD4, CD8, CD25, IL-2, IFN-γ and TNF-α) were then added to the solution. The Facial Action Coding System was used to determine the positive rates of the cells combined with the monoclonal antibodies above. No significant statistical differences were observed between the CEN, NC and AG groups. However, some data of the FN group were significantly higher than those of the other groups at the corresponding time. No obvious immune rejections were observed among the chemically extracted acellular nerve allografts compared with fresh nerve autograft.
View References
1
|
AJ AguayoK MizunoGM BraySchwann cell
transplantation: evidence for a primary sheath cell disorder
causing hypomyelination in quaking miceJ Neuropath Exp
Neurol36595197710.1097/00005072-197705000-00031
|
2
|
AJ AguayoGM BrayJ KasarjianDifferences in
myelination of mouse axons by transplanted human and mouse Schwann
cellsNeurology283561978
|
3
|
AJ AguayoGM BraySC PerkinsAxon-Schwann
cell relationships in neuropathies of mutant miceAnn NY Acad
Sci317512531197910.1111/j.1749-6632.1979.tb37386.x289329
|
4
|
PJ EvansR MidhaSE MackinnonThe peripheral
nerve allograft: a comprehensive review of regeneration and
neuroimmunologyProg
Neurobiol43187233199410.1016/0301-0082(94)90001-97816927
|
5
|
ME EsiriMC ReadingMacrophages, lymphocytes
and major histocompatibility complex class II antigens in adult
human sensory and sympathetic
gangliaNeurology2318719319892754017
|
6
|
E ScarpiniRP LisakS BerettaQuantitative
assessment of class II molecules in normal and pathological
nervesBrain113659675199010.1093/brain/113.3.6592163718
|
7
|
T TrumbleJ StanislawImmunology of
peripheral nerves and response to traumaJ Orthop
Res9367373199110.1002/jor.11000903082010840
|
8
|
K BergsteinsdottirA KingstonKR IessenRat
Schwann cells can be induced to express major histocompatibility
complex class II molecules in vivoJ
Neurocytol21382390199210.1007/BF011917061607881
|
9
|
YL ColsonBH MarkusA ZeeviRJ
DuquesnoyIncreased lymphocyte adherence to human arterial
endothelial cell monolayers in the context of allorecognitionJ
Immunol1442975298419901691225
|
10
|
P HayryR RenkonenD LeszczynskiLocal events
in graft rejectionTransplant Proc213716372019892669283
|
11
|
ML RoseC PageC HengstenbergMH
YacoubIdentification of antigen presenting cells in normal and
transplanted human heart: importance of endothelial cellsHuman
Immunol28179185199010.1016/0198-8859(90)90017-J1972151
|
12
|
JP TurunenJ HalttunenP HäyryR
RenkonenLymphocytes bind to capillary endothelium during heart
allograft rejectionTransplant Proc2212619902309281
|
13
|
LT YuWF HickeyWS SilversD LaRossaAM
RostamiExpression of class II antigens on peripheral nerve
allograftsAnn NY Acad
Sci540472474198810.1111/j.1749-6632.1988.tb27139.x3207279
|
14
|
F LassnerE SchallerG SteinhoffCellular
mechanisms of rejection and regeneration in peripheral nerve
allograftsTransplantation48386392198910.1097/00007890-198909000-000062781604
|
15
|
JS PoberRS CotranThe role of endothelial
cells in
inflammationTransplantation50537544199010.1097/00007890-199010000-00001
|
16
|
M SondellG LundborgM KanjeRegeneration of
the rat sciatic nerve into allografts acellular through chemical
extractionBrain
Res7954454199810.1016/S0006-8993(98)00251-09622591
|
17
|
AJ AguayoSC PerkinsGM BrayPersistence of
abnormal myelination 4 months after transplantation of Schwann
cells from trembler to normal mouse nervesNeurology273771977
|
18
|
MC HorowitzGE FriedlaendImmunologic
aspects of bone transplantationOrthop Clin North
Am1822719872951639
|
19
|
T JungU SchauerC HeusserC NeumannC
RiegerDetection of intracellular cytokines by flow cytometryJ
Immunol Methods159197207199310.1016/0022-1759(93)90158-48445253
|
20
|
B SanderJ AnderssonU AnderssonAssessment
of cytokines by immunofluorescence and the paraformal
dehyde-saponin procedureImmunol
Rev1196593199110.1111/j.1600-065X.1991.tb00578.x2045123
|