Steroid receptor coactivator-3 differentially regulates the inflammatory response in peritoneal macrophages

Li,Jun; Liu,Ying-Hai; Ou,Shan; Dai,Xue-Mei; Wang,Jun-Ping; Su,Yong-Ping

doi:10.3892/mmr.2012.750

April 2012 Volume 5 Issue 4

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April 2012 Volume 5 Issue 4

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Article

Steroid receptor coactivator-3 differentially regulates the inflammatory response in peritoneal macrophages

Authors:
- Jun Li
- Ying-Hai Liu
- Shan Ou
- Xue-Mei Dai
- Jun-Ping Wang
- Yong-Ping Su
View Affiliations / Copyright

Affiliations: Department of Anesthesia, General Hospital of Chengdu Military Command Area, Chengdu, Sichuan 610083, P.R. China, Institute of Combined Injury of PLA, State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing 400038, P.R. China
Pages: 1099-1105
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Published online on: January 12, 2012

https://doi.org/10.3892/mmr.2012.750
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Abstract

Steroid receptor coactivator-3 (SRC-3) is a transcriptional coactivator that plays an important role in the regulation of cytokine mRNA translation. In the present study, SCR-3 gene knockout mice were used to study the effects of SCR-3 on the regulation of the inflammatory response in peritoneal macrophages induced by lipopolysaccharides (LPS). Peritoneal macrophages (PMs) of SRC-3-/- mice showed a decrease in the release of TNF-α, IL-1β and IL-6, and an increase in the release of IL-10. Furthermore, results of RT-PCR also showed that levels of TNF-α, IL-1β and IL-6 mRNA expression were significantly lower, while the level of IL-10 mRNA expression was higher in the SRC-3-/- mice, compared to those of wild-type mice, following treatment with LPS (p﹤0.01). In addition, western blotting revealed that: i) the extent of reduction of the glucocorticoid receptor in PMs from SRC-3-/- mice was significantly lower than that in wild-type mice (p﹤0.01); ii) the extent of increase of AP-1 in PMS from SRC-3-/- mice was significantly lower than that in wild-type mice (p﹤0.01); iii) the extent of increase of NF-κB p65 in PMs from SRC-3-/- mice was significantly higher than that in wild-type mice (p﹤0.01). Collectively, our studies revealed that SRC-3 may play a key role in the maintenance of innate immunity. Furthermore, absence of the SRC-3 protein may result in the partial loss of inflammation and phagocytosis barrier function, including suppression of LPS-induced transcriptional activity, release of TNF-α, IL-1β and IL-6, and obstruction of the function of phagocytes and elimination of bacteria, as well as their production.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Steroid receptor coactivator-3 differentially regulates the inflammatory response in peritoneal macrophages

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