Tanshinone IIA inhibits human breast cancer MDA-MB-231 cells by decreasing LC3-II, Erb-B2 and NF-κBp65
- Authors:
- Chin-Cheng Su
- Su-Yu Chien
- Shou-Jen Kuo
- Yao-Li Chen
- Chun-Yuan Cheng
- Dar-Ren Chen
View Affiliations
Affiliations: Department of Surgery, Comprehensive Breast Cancer Center, Laboratory of Integrative Medicine Cancer Research, Changhua Christian Hospital, Changhua 500-06, Taiwan, R.O.C., Department of Pharmacy, Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 500-06, Taiwan, R.O.C., Department of Surgery, Comprehensive Breast Cancer Center, Changhua Christian Hospital, Changhua 500-06, Taiwan, R.O.C.
- Published online on: January 13, 2012 https://doi.org/10.3892/mmr.2012.756
-
Pages:
1019-1022
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Abstract
The ability of tanshinone IIA (Tan-IIA) to inhibit the proliferation of human breast cancer cell lines in vitro and in vivo is well documented. However, the molecular mechanisms have not been fully elucidated. In the present study, MDA-MB-231 cells were treated with different concentrations of Tan-IIA for 48 h, followed by protein extraction for western blotting. For an in vivo study, MDA-MB-231 cells were implanted directly into female SCID mice which were divided randomly into three groups to be treated with vehicle, Tan-IIA (20 mg/kg) and Tan-IIA (60 mg/kg) every other day orally, with treatment starting 4 weeks after inoculation with the MDA-MB-231 cells. The results showed that Tan-IIA inhibited the proliferation of MDA-MB-231 cells and decreased the protein expression of LC3-II and Erb-B2 in vitro. Treatment with Tan-IIA (20 or 60 mg/kg) for 90 days resulted in a reduction in tumor size and weight compared to the control group. The protein expression of NF-κBp65 was reduced, while caspase-3 was up-regulated compared to the control group. These findings indicate that Tan-IIA inhibits tumor growth in a MDA-MB-231 xenograft animal model. One of the molecular mechanisms may be through a decrease in NF-κBp65 and an increase in caspase-3 expression.
View References
|
1
|
A JemalF BrayMM CenterJ FerlayE WardD
FormanGlobal Cancer StatisticsCA Cancer J
Clin616990201110.3322/caac.20107
|
|
2
|
TW LeungPJ JohnsonSystemic therapy for
hepatocellular carcinomaSemin
Oncol28514520200110.1016/S0093-7754(01)90144-711685744
|
|
3
|
ZT LiBJ YangGE MaChemical studies of
Salvia miltiorrhiza f. albaYaoxue Xuebao262092131991(In
Chinese).
|
|
4
|
TJ LinAntioxidation mechanism of
schizandrin and tanshinonatic acid A and their effects on the
protection of cardio-toxic action of adriamycinShengli Kexue
Jinzhan223423451991(In Chinese).
|
|
5
|
Y LiangYM YangSL YuanStudies on Pharmic
mechanism and clinic application of TanshinoneTradit Herbal
Drugs313043062000
|
|
6
|
CC SuYH LinTanshinone IIA down-regulates
the protein expression of ErbB-2 and up-regulates TNF-α in colon
cancer cells in vitro and in vivoInt J Mol
Med22847851200819020785
|
|
7
|
CC SuGW ChenJC KangMH ChanGrowth
inhibition and apoptosis induction by tanshinone IIA in human colon
adenocarcinoma cellsPlanta
Med7413571362200810.1055/s-2008-108129918622903
|
|
8
|
CY ChengCC SuTanshinone IIA inhibits
Hep-J5 cells by increasing calreticulin, caspase 12 and GADD153
protein expressionInt J Mol Med26379385201020664954
|
|
9
|
CY ChengCC SuTanshinone IIA may inhibit
the growth of small cell lung cancer H146 cells by up-regulating
the Bax/Bcl-2 ratio and decreasing mitochondrial membrane
potentialMol Med Rep3645650201021472292
|
|
10
|
TL ChiuCC SuTanshinone IIA induces
apoptosis in human lung cancer A549 cells through the induction of
reactive oxygen species and decreasing the mitochondrial membrane
potentialInt J Mol Med25231236201020043132
|
|
11
|
CC SuYH LinTanshinone IIA inhibits human
breast cancer cells through increased Bax to Bcl-xL ratiosInt J Mol
Med22357361200818698495
|
|
12
|
X ZhangPR ZhangJ ChenQA LüStudy on the
effect of Tanshinone II A against human breast cancer in
vivoSichuan Da Xue Xue Bao Yi Xue Ban4162672010(In Chinese).
|
|
13
|
Q LuP ZhangX ZhangJ ChenExperimental study
of the anti-cancer mechanism of tanshinone IIA against human breast
cancerInt J Mol Med24773780200919885617
|
|
14
|
PR ZhangQA Lüstudy on anticancer activity
of tanshinone IIA against human breast cancerSichuan Da Xue Xue Bao
Yi Xue Ban402452492009(In Chinese).
|
|
15
|
X WangY WeiS YuanG LiuY LuJ ZhangW
WangPotential anticancer activity of tanshinone IIA against human
breast cancerInt J Cancer116799807200510.1002/ijc.2088015849732
|
|
16
|
HC ChenWT HsiehWC ChangJG ChungAloe-emodin
induced in vitro G2/M arrest of cell cycle in human promyelocytic
leukemia HL-60 cellsFood Chem
Toxicol4212511257200410.1016/j.fct.2004.03.00215207375
|
|
17
|
MM BradfordA rapid and sensitive method
for the quantitation of microgram quantities of protein using the
principle of protein-dye bindingAnal
Biochem72248254197610.1016/0003-2697(76)90527-3942051
|
