Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April 2012 Volume 5 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April 2012 Volume 5 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation

  • Authors:
    • Joan Gannon
    • Kay Ohlendieck
  • View Affiliations / Copyright

    Affiliations: Laboratory of Systems Medicine and Cell Biology, Department of Medicine, Research Institute of McGill University Health Centre, Montreal, QC, Canada, Muscle Biology Laboratory, Department of Biology, National University of Ireland, Maynooth, Republic of Ireland
  • Pages: 993-1000
    |
    Published online on: January 17, 2012
       https://doi.org/10.3892/mmr.2012.759
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The progressive loss of skeletal muscle mass is a serious pathophysiological problem in the elderly, which warrants detailed biochemical studies into the underlying mechanism of age-related fiber degeneration. Over the last few years, mass spectrometry (MS)-based proteomics has identified a considerable number of new biomarkers of muscle aging in humans and animal models of sarcopenia. However, interpretation of the proteomic findings is often complicated by technical and biological limitations. Although gel electrophoresis-based approaches represent a highly sensitive analytical way for the large-scale and high-throughput survey of global changes in skeletal muscle proteins during aging, often the presence of components with an isoelectric point in the basic range is underestimated. We, therefore, carried out a comparative subproteomic study of young versus aged rat muscle focusing on potential changes in muscle proteins with an alkaline isoelectric point, using a combination of offgel electrophoresis and two-dimensional (2D) slab gel electrophoresis. Offgel electrophoresis was successfully applied as a prefractionation step to enrich basic protein species from crude tissue extracts representing young adult versus senescent muscle specimens. Proteomics has demonstrated alterations in a small cohort of basic proteins during muscle aging. The mass spectrometric identification of altered proteins and immunoblotting revealed a decrease in the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and a concomitant increase in mitochondrial creatine kinase (CK) and ubiquinol cytochrome‑c reductase. This agrees with the idea of a glycolytic-to-oxidative shift during muscle aging, which is indicative of an overall fast-to-slow transition process in senescent rat muscle. Thus, alterations in the abundance of metabolic enzymes appear to play a central role in the molecular pathogenesis of age‑dependent muscle wasting.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

DR ThomasSarcopeniaClin Geriatr Med26331346201010.1016/j.cger.2010.02.012

2 

AA VandervoortAging of the human neuromuscular systemMuscle Nerve251725200210.1002/mus.121511754180

3 

LV ThompsonAge-related muscle dysfunctionExp Gerontol44106111200910.1016/j.exger.2008.05.00318657920

4 

JA FaulknerLM LarkinDR ClaflinSV BrooksAge-related changes in the structure and function of skeletal musclesClin Exp Pharmacol Physiol3410911096200710.1111/j.1440-1681.2007.04752.x17880359

5 

E EdstromM AltunE BergmanH JohnsonS KullbergV Ramirez-LeonB UlfhakeFactors contributing to neuromuscular impairment and sarcopenia during agingPhysiol Behav92129135200710.1016/j.physbeh.2007.05.04017585972

6 

JG RyallJD SchertzerGS LynchCellular and molecular mechanisms underlying age-related skeletal muscle wasting and weaknessBiogerontology9213228200810.1007/s10522-008-9131-018299960

7 

WR FronteraKF ReidEM PhillipsLS KrivickasVA HughesR RoubenoffRA FieldingMuscle fiber size and function in elderly humans: a longitudinal studyJ Appl Physiol105637642200810.1152/japplphysiol.90332.200818556434

8 

MC KostekMJ DelmonicoAge-related changes in adult muscle morphologyCurr Aging SciApril292011(Epub ahead of print)

9 

AA VandervoortTB SymonsFunctional and metabolic consequences of sarcopeniaCan J Appl Physiol2690101200110.1139/h01-00711173671

10 

JS KimJM WilsonSR LeeDietary implications on mechanisms of sarcopenia: roles of protein, amino acids and antioxidantsJ Nutr Biochem21113201010.1016/j.jnutbio.2009.06.01419800212

11 

Y RollandC DupuyG Abellan van KanS GilletteB VellasTreatment strategies for sarcopenia and frailtyMed Clin North Am95427438201110.1016/j.mcna.2011.02.00821549870

12 

BF HurleyED HansonAK SheaffStrength training as a countermeasure to aging muscle and chronic diseaseSports Med41289306201110.2165/11585920-000000000-0000021425888

13 

M PahorS KritchevskyResearch hypotheses on muscle wasting, aging, loss of function and disabilityJ Nutr Health Aging297100199810993575

14 

DR ThomasLoss of skeletal muscle mass in aging: examining the relationship of starvation, sarcopenia and cachexiaClin Nutr26389399200710.1016/j.clnu.2007.03.00817499396

15 

JE MorleyDiabetes, sarcopenia, and frailtyClin Geriatr Med24455469200810.1016/j.cger.2008.03.004

16 

P DoranJ GannonK O’ConnellK OhlendieckProteomic profiling of animal models mimicking skeletal muscle disordersProteomics Clin Appl111691184200710.1002/prca.20070004221136766

17 

P DoranP DonoghueK O’ConnellJ GannonK OhlendieckProteomics of skeletal muscle agingProteomics99891003200910.1002/pmic.20080036519180535

18 

J KanskiSJ HongC SchoneichProteomic analysis of protein nitration in aging skeletal muscle and identification of nitrotyrosine-containing sequences in vivo by nanoelectrospray ionization tandem mass spectrometryJ Biol Chem2802426124266200510.1074/jbc.M501773200

19 

J GannonL StauntonK O’ConnellP DoranK OhlendieckPhosphoproteomic analysis of aged skeletal muscleInt J Mol Med2233422008

20 

J FengH XieDL MeanyLV ThompsonEA ArriagaTJ GriffinQuantitative proteomic profiling of muscle type-dependent and age-dependent protein carbonylation in rat skeletal muscle mitochondriaJ Gerontol A Biol Sci Med Sci6311371152200810.1093/gerona/63.11.113719038828

21 

K O’ConnellP DoranJ GannonK OhlendieckLectin-based proteomic profiling of aged skeletal muscle: decreased pyruvate kinase isozyme M1 exhibits drastically increased levels of N-glycosylationEur J Cell Biol877938052008

22 

I PiecA ListratJ AlliotC ChambonRG TaylorD BechetDifferential proteome analysis of aging in rat skeletal muscleFASEB J1911431145200515831715

23 

C GelfiA ViganoM RipamontiA PontoglioS BegumMA PellegrinoB GrassiR BottinelliR WaitP CerretelliThe human muscle proteome in agingJ Proteome Res513441353200610.1021/pr050414x16739986

24 

K O’ConnellJ GannonP DoranK OhlendieckProteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscleInt J Mol Med20145153200717611631

25 

P DoranJ GannonK O’ConnellK OhlendieckAging skeletal muscle shows a drastic increase in the small heat shock proteins alphaB-crystallin/HspB5 and cvHsp/HspB7Eur J Cell Biol86629640200710.1016/j.ejcb.2007.07.003

26 

P DoranK O’ConnellJ GannonM KavanaghK OhlendieckOpposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysisProteomics8364377200810.1002/pmic.20070047518050275

27 

D CapitanioM VassoC FaniaM MoriggiA ViganoP ProcacciV MagnaghiC GelfiComparative proteomic profile of rat sciatic nerve and gastrocnemius muscle tissues in ageing by 2-D DIGEProteomics920042020200910.1002/pmic.20070116219333999

28 

J GannonP DoranA KirwanK OhlendieckDrastic increase of myosin light chain MLC-2 in senescent skeletal muscle indicates fast-to-slow fibre transition in sarcopenia of old ageEur J Cell Biol88685700200910.1016/j.ejcb.2009.06.00419616867

29 

P DonoghueL StauntonE MullenG ManningK OhlendieckDIGE analysis of rat skeletal muscle proteins using nonionic detergent phase extraction of young adult versus aged gastrocnemius tissueJ Proteomics7314411453201010.1016/j.jprot.2010.01.014

30 

K O’ConnellK OhlendieckProteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscleProteomics955095524200919834913

31 

I BraticA TrifunovicMitochondrial energy metabolism and ageingBiochim Biophys Acta1797961967201010.1016/j.bbabio.2010.01.00420064485

32 

G PariseM De LisioMitochondrial theory of aging in human age-related sarcopeniaInterdiscip Top Gerontol37142156201010.1159/00031999920703060

33 

L StauntonK O’ConnellK OhlendieckProteomic profiling of mitochondrial enzymes during skeletal muscle agingJ Aging Res2011908035201110.4061/2011/90803521437005

34 

PG RighettiA CastagnaP AntonioliE BoschettiPrefractio nation techniques in proteome analysis: the mining tools of the third millenniumElectrophoresis26297319200510.1002/elps.20040618915657944

35 

P HorthCA MillerT PreckelC WenzEfficient fractionation and improved protein identification by peptide OFFGEL electrophoresisMol Cell Proteomics519681974200610.1074/mcp.T600037-MCP20016849286

36 

EM KeidelD DoschA BrunnerJ KellermannF LottspeichEvaluation of protein loading techniques and improved separation in OFFGEL isoelectric focusingElectrophoresis3216591666201121563181

37 

S FratermanU ZeigerTS KhuranaNA RubinsteinM WilmCombination of peptide OFFGEL fractionation and label-free quantitation facilitated proteomics profiling of extraocular muscleProteomics734043416200710.1002/pmic.20070038217708596

38 

M ChevalletS LucheT RabilloudSilver staining of proteins in polyacrylamide gelsNat Protoc118521858200610.1038/nprot.2006.28817487168

39 

HM McBrideM NeuspielS WasiakMitochondria: more than just a powerhouseCurr Biol16R551R560200610.1016/j.cub.2006.06.05416860735

40 

DC ChanMitochondria: dynamic organelles in disease, aging, and developmentCell12512411252200610.1016/j.cell.2006.06.01016814712

41 

DA HoodI IrrcherV LjubicicAM JosephCoordination of metabolic plasticity in skeletal muscleJ Exp Biol20922652275200610.1242/jeb.0218216731803

42 

U SchlattnerM Tokarska-SchlattnerT WallimannMitochondrial creatine kinase in human health and diseaseBiochim Biophys Acta1762164180200610.1016/j.bbadis.2005.09.00416236486

43 

NV DudkinaR KourilK PetersHP BraunEJ BoekemaStructure and function of mitochondrial supercomplexesBiochim Biophys Acta1797664670201010.1016/j.bbabio.2009.12.01320036212

44 

K OhlendieckProteomics of skeletal muscle glycolysisBiochim Biophys Acta180420892101201010.1016/j.bbapap.2010.08.00120709194

45 

A HionaC LeeuwenburghThe role of mitochondrial DNA mutations in aging and sarcopenia: implications for the mitochondrial vicious cycle theory of agingExp Gerontol432433200810.1016/j.exger.2007.10.00117997255

46 

B ChabiV LjubicicKJ MenziesJH HuangA SaleemDA HoodMitochondrial function and apoptotic susceptibility in aging skeletal muscleAging Cell7212200810.1111/j.1474-9726.2007.00347.x18028258

47 

PA FigueiredoSK PowersRM FerreiraHJ AppellJA DuarteAging impairs skeletal muscle mitochondrial bioenergetic functionJ Gerontol A Biol Sci Med Sci642133200910.1093/gerona/gln04819196905

48 

D PetteThe adaptive potential of skeletal muscle fibersCan J Appl Physiol27423448200210.1139/h02-02312442355

49 

P DonoghueP DoranK WynneK PedersenMJ DunnK OhlendieckProteomic profiling of chronic low-frequency stimulated fast muscleProteomics734173430200710.1002/pmic.20070026217708595

50 

L StauntonH JockuschC WiegandT AlbrechtK OhlendieckIdentification of secondary effects of hyperexcitability by proteomic profiling of myotonic mouse muscleMol Biosyst724802489201110.1039/c1mb05043e21629954

51 

MA AlnaqeebG GoldspinkChanges in fiber type, number and diameter in developing and ageing skeletal muscleJ Anat153314519873429325

52 

MR DeschenesEffects of aging on muscle fiber type and sizeSports Med34809824200410.2165/00007256-200434120-0000215462613

53 

J LexellCC TaylorM SjostromWhat is the cause of the ageing atrophy? Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15- to 83-year-old menJ Neurol Sci842752941988

54 

J LexellHuman aging, muscle mass, and fiber type compositionJ Gerontol A Biol Sci Med Sci5011161995

55 

O DelbonoExpression and regulation of excitation-contraction coupling proteins in aging skeletal muscleCurr Aging SciApril292011(Epub ahead of print)

56 

E ProchniewiczLV ThompsonDD ThomasAge-related decline in actomyosin structure and functionExp Gerontol42931938200710.1016/j.exger.2007.06.01517706387

57 

CE LeeA McArdleRD GriffithsThe role of hormones, cytokines and heat shock proteins during age-related muscle lossClin Nutr26524534200710.1016/j.clnu.2007.05.00517590243

58 

E MarzettiC LeeuwenburghSkeletal muscle apoptosis, sarcopenia and frailty at old ageExp Gerontol4112341238200610.1016/j.exger.2006.08.01117052879

59 

DW RussIR LanzaThe impact of old age on skeletal muscle energetics: supply and demandCurr Aging Sci2011Apr29(in press)

60 

K O’ConnellJ GannonP DoranK OhlendieckReduced expression of sarcalumenin and related Ca2+-regulatory proteins in aged rat skeletal muscleExp Gerontol43958961200818762239

61 

C BruleE DargelosR DialloA ListratD BechetP CottinS PoussardProteomic study of calpain interacting proteins during skeletal muscle agingBiochimie9219231933201010.1016/j.biochi.2010.09.00320850499

62 

P LorenzonE BandiF de GuarriniT PietrangeloR SchaferM ZweyerA WernigF RuzzierAgeing affects the differentiation potential of human myoblastsExp Gerontol3915451554200410.1016/j.exger.2004.07.00815501025

63 

AC KayaniJP MortonA McArdleThe exercise-induced stress response in skeletal muscle: failure during agingAppl Physiol Nutr Metab3310331041200810.1139/H08-08918923581

64 

T LangT StreeperP CawthonK BaldwinDR TaaffeTB HarrisSarcopenia: etiology, clinical consequences, intervention, and assessmentOsteoporos Int21543559201010.1007/s00198-009-1059-y19779761

65 

AJ Cruz-JentoftJP BaeyensJM BauerY BoirieT CederholmF LandiFC MartinJP MichelY RollandSM SchneiderSarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older peopleAge Ageing39412423201010.1093/ageing/afq03420392703

66 

AJ Cruz-JentoftF LandiE TopinkovaJP MichelUnderstanding sarcopenia as a geriatric syndromeCurr Opin Clin Nutr Metab Care1317201010.1097/MCO.0b013e328333c1c119915458

67 

M PahorT ManiniM CesariSarcopenia: clinical evaluation, biological markers and other evaluation toolsJ Nutr Health Aging13724728200910.1007/s12603-009-0204-919657557

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Gannon J and Ohlendieck K: Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Mol Med Rep 5: 993-1000, 2012.
APA
Gannon, J., & Ohlendieck, K. (2012). Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Molecular Medicine Reports, 5, 993-1000. https://doi.org/10.3892/mmr.2012.759
MLA
Gannon, J., Ohlendieck, K."Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation". Molecular Medicine Reports 5.4 (2012): 993-1000.
Chicago
Gannon, J., Ohlendieck, K."Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation". Molecular Medicine Reports 5, no. 4 (2012): 993-1000. https://doi.org/10.3892/mmr.2012.759
Copy and paste a formatted citation
x
Spandidos Publications style
Gannon J and Ohlendieck K: Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Mol Med Rep 5: 993-1000, 2012.
APA
Gannon, J., & Ohlendieck, K. (2012). Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation. Molecular Medicine Reports, 5, 993-1000. https://doi.org/10.3892/mmr.2012.759
MLA
Gannon, J., Ohlendieck, K."Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation". Molecular Medicine Reports 5.4 (2012): 993-1000.
Chicago
Gannon, J., Ohlendieck, K."Subproteomic analysis of basic proteins in aged skeletal muscle following offgel pre-fractionation". Molecular Medicine Reports 5, no. 4 (2012): 993-1000. https://doi.org/10.3892/mmr.2012.759
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team