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April 2012 Volume 5 Issue 4

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Article

Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1

  • Authors:
    • Huaxing Luo
    • Yingxue Hao
    • Bo Tang
    • Dongzhu Zeng
    • Yan Shi
    • Peiwu Yu
  • View Affiliations / Copyright

    Affiliations: General Surgery Center, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 988-992
    |
    Published online on: February 3, 2012
       https://doi.org/10.3892/mmr.2012.777
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Abstract

Peritoneal implantation metastasis of gastric cancer cells is associated with poor prognosis. Peritoneal macrophages are the most important immune cells in the abdominal cavity to control tumor metastasis. In the present study, the immunosuppressive effects of mouse forestomach cells on macrophages were examined. Conditioned medium from mouse forestomach cell cultures were used to treat isolated peritoneal macrophages. A colorimetry-based phagocytosis assay was performed to investigate the functional change of macrophages. The alteration of cytokine secretion by macrophages was measured by ELISA assay. Specific markers of macrophage polarization were analyzed by real-time RT-PCR. TGF-β1 signaling was evaluated by western blotting. Neutralization experiments were performed using an anti-TGF-β1 antibody. Conditioned medium reduced the phagocytotic capability of macrophages. Lower TNF-α and IL-1β levels and higher IL-10 and VEGF levels were observed. Real-time RT-PCR showed increased mRNA levels of M2 macrophage markers. Further study revealed that TGF-β1 was significantly elevated in the conditioned medium and TGF-β1 signaling was activated in the macrophages by the treatment of conditioned medium. Neutralization of TGF-β1 reversed the immunosuppressive effects on macrophages. Immunosuppressive macrophages can be induced by conditioned medium from mouse forestomach cell cultures. These effects appeared to occur through the production of TGF-β1 by the tumor cells. Targeted TGF-β1 intervention may help to control peritoneal metastasis of gastric cancers.
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Copy and paste a formatted citation
Spandidos Publications style
Luo H, Hao Y, Tang B, Zeng D, Shi Y and Yu P: Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1. Mol Med Rep 5: 988-992, 2012.
APA
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., & Yu, P. (2012). Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1. Molecular Medicine Reports, 5, 988-992. https://doi.org/10.3892/mmr.2012.777
MLA
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., Yu, P."Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1". Molecular Medicine Reports 5.4 (2012): 988-992.
Chicago
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., Yu, P."Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1". Molecular Medicine Reports 5, no. 4 (2012): 988-992. https://doi.org/10.3892/mmr.2012.777
Copy and paste a formatted citation
x
Spandidos Publications style
Luo H, Hao Y, Tang B, Zeng D, Shi Y and Yu P: Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1. Mol Med Rep 5: 988-992, 2012.
APA
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., & Yu, P. (2012). Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1. Molecular Medicine Reports, 5, 988-992. https://doi.org/10.3892/mmr.2012.777
MLA
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., Yu, P."Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1". Molecular Medicine Reports 5.4 (2012): 988-992.
Chicago
Luo, H., Hao, Y., Tang, B., Zeng, D., Shi, Y., Yu, P."Mouse forestomach carcinoma cells immunosuppress macrophages through transforming growth factor-β1". Molecular Medicine Reports 5, no. 4 (2012): 988-992. https://doi.org/10.3892/mmr.2012.777
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