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Molecular Medicine Reports
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Print ISSN: 1791-2997 Online ISSN: 1791-3004
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April 2012 Volume 5 Issue 4

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Article

Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma

  • Authors:
    • Bo Zou
    • Shoujuan Sun
    • Xiaojuan Qi
    • Ping Ji
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Chongqing Medical University, ChongQing, P.R. China
  • Pages: 1116-1120
    |
    Published online on: February 3, 2012
       https://doi.org/10.3892/mmr.2012.781
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Abstract

Accumulating data reveal that aldehyde dehydrogenase (ALDH) activity is a cancer stem cell marker in several types of human cancers. Whether ALDH also defines cancer stem cells of tongue squamous cell carcinoma is unknown. In the present study, we performed the Aldefluor assay to detect ALDH enzymatic activity, and used flow cytometry to isolate ALDH+ and ALDH- cells based on their ALDH activity in the Tca8113 tongue squamous cell carcinoma cell line. We found that Tca8113 cells harbored 1.3% of ALDH+ cells, which exhibited higher proliferation capacity than their ALDH- counterparts and parental cells. Sorted ALDH+ cells were able to differentiate and generate ALDH- cells in vitro. Moreover, in serum-free medium, ALDH+, but not ALDH- cells, survived and formed tumorspheres. The suppression subtractive hybridization assay revealed 68 overexpressed or underexpressed genes in the ALDH+ subpopulation relative to ALDH- cells, which included a set of known cancer stem cell-related genes. Thus, our data demonstrated that a small subset of Tca8113 cells with high ALDH enzymatic activity display characteristics of cancer stem cells, suggesting that ALDH activity may be a cancer stem cell marker for tongue squamous cell carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Zou B, Sun S, Qi X and Ji P: Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma. Mol Med Rep 5: 1116-1120, 2012.
APA
Zou, B., Sun, S., Qi, X., & Ji, P. (2012). Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma. Molecular Medicine Reports, 5, 1116-1120. https://doi.org/10.3892/mmr.2012.781
MLA
Zou, B., Sun, S., Qi, X., Ji, P."Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma". Molecular Medicine Reports 5.4 (2012): 1116-1120.
Chicago
Zou, B., Sun, S., Qi, X., Ji, P."Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma". Molecular Medicine Reports 5, no. 4 (2012): 1116-1120. https://doi.org/10.3892/mmr.2012.781
Copy and paste a formatted citation
x
Spandidos Publications style
Zou B, Sun S, Qi X and Ji P: Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma. Mol Med Rep 5: 1116-1120, 2012.
APA
Zou, B., Sun, S., Qi, X., & Ji, P. (2012). Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma. Molecular Medicine Reports, 5, 1116-1120. https://doi.org/10.3892/mmr.2012.781
MLA
Zou, B., Sun, S., Qi, X., Ji, P."Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma". Molecular Medicine Reports 5.4 (2012): 1116-1120.
Chicago
Zou, B., Sun, S., Qi, X., Ji, P."Aldehyde dehydrogenase activity is a cancer stem cell marker of tongue squamous cell carcinoma". Molecular Medicine Reports 5, no. 4 (2012): 1116-1120. https://doi.org/10.3892/mmr.2012.781
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