Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March 2013 Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March 2013 Volume 7 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells

  • Authors:
    • Yiming Qi
    • Ying Li
    • Lin Zhang
    • Junqi Huang
  • View Affiliations / Copyright

    Affiliations: Institute of Immunology, Zhongshan School of Medicine, Sun Yat‑sen University, Guangzhou 510080, P.R. China, Key Laboratory of Tropical Diseases Control, Ministry of Education, Guangzhou 510080, P.R. China
  • Pages: 791-798
    |
    Published online on: January 24, 2013
       https://doi.org/10.3892/mmr.2013.1288
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Dengue virus (DENV) causes self‑limiting dengue fever (DF), severe dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). It is generally considered that cytokine storm leads to the increased plasma leakage characteristic of DHF/DSS. In the present study, peripheral blood mononuclear cells (PBMCs) were isolated from blood samples of healthy volunteers and infected with DENV serotype 2 (DENV2). Culture supernatants of DENV2‑infected and -uninfected PBMCs were analyzed using a human cytokine array. Between a 6‑12 h post‑infection, levels of CCL5, IL‑6 and IL‑8 were markedly elevated, while those of TNF‑α decreased. Total RNA isolated from these PBMCs was analyzed by human miRNA microarray to identify differentially expressed microRNAs (miRNAs). Quantitative reverse transcription polymerase chain reaction was used to validate 11 upregulated and 4 downregulated miRNAs. Sanger mibase, miRanda and TargetScan were used to identify 261 common predicted genes. Databases were used to identify homologous sequences on mRNAs of putative target genes that may be directly bound by the miRNAs identified. We found that cytokines and epigenetic regulators may be putative target genes of these miRNAs. Using ingenuity pathway analysis, we noted that canonical pathways, including biological regulation, may be modulated by these miRNAs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Guzman MG and Vazquez S: The complexity of antibody-dependent enhancement of dengue virus infection. Viruses. 2:2649–2662. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Martina BE, Koraka P and Osterhaus AD: Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev. 22:564–581. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Green S and Rothman A: Immunopathological mechanisms in dengue and dengue hemorrhagic fever. Curr Opin Infect Dis. 19:429–436. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Pang T, Cardosa MJ and Guzman MG: Of cascades and perfect storms: the immunopathogenesis of dengue haemorrhagic fever-dengue shock syndrome (DHF/DSS). Immunol Cell Biol. 85:43–45. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Gracias DT and Katsikis PD: MicroRNAs: key components of immune regulation. Adv Exp Med Biol. 780:15–26. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Lieber D and Haas J: Viruses and microRNAs: a toolbox for systematic analysis. Wiley Interdiscip Rev RNA. 2:787–801. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Curtale G, Citarella F, Carissimi C, et al: An emerging player in the adaptive immune response: microRNA-146a is a modulator of IL-2 expression and activation-induced cell death in T lymphocytes. Blood. 115:265–273. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Hashimi ST, Fulcher JA, Chang MH, Gov L, Wang S and Lee B: MicroRNA profiling identifies miR-34a and miR-21 and their target genes JAG1 and WNT1 in the coordinate regulation of dendritic cell differentiation. Blood. 114:404–414. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Ceppi M, Pereira PM, Dunand-Sauthier I, et al: MicroRNA-155 modulates the interleukin-1 signaling pathway in activated human monocyte-derived dendritic cells. Proc Natl Acad Sci USA. 106:2735–2740. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Sato F, Tsuchiya S, Meltzer SJ and Shimizu K: MicroRNAs and epigenetics. FEBS J. 278:1598–1609. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Liao H, Xu J and Huang J: FasL/Fas pathway is involved in dengue virus induced apoptosis of the vascular endothelial cells. J Med Virol. 82:1392–1399. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Neseliler S, Narayanan D, Fortis-Santiago Y, Katz DB and Birren SJ: Genetically induced cholinergic hyper-innervation enhances taste learning. Front Syst Neurosci. 5:972011. View Article : Google Scholar : PubMed/NCBI

13 

Luers AJ, Loudig OD and Berman JW: MicroRNAs are expressed and processed by human primary macrophages. Cell Immunol. 263:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Espada-Murao LA and Morita K: Dengue and soluble mediators of the innate immune system. Trop Med Health. 39:53–62. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Nguyen TH, Nguyen TL, Lei HY, et al: Association between sex, nutritional status, severity of dengue hemorrhagic fever and immune status in infants with dengue hemorrhagic fever. Am J Trop Med Hyg. 72:370–374. 2005.PubMed/NCBI

16 

Chakravarti A and Kumaria R: Circulating levels of tumour necrosis factor-alpha and interferon-gamma in patients with dengue and dengue haemorrhagic fever during an outbreak. Indian J Med Res. 123:25–30. 2006.PubMed/NCBI

17 

Perez AB, Garcia G, Sierra B, et al: IL-10 levels in Dengue patients: some findings from the exceptional epidemiological conditions in Cuba. J Med Virol. 73:230–234. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Juffrie M, Meer GM, Hack CE, et al: Inflammatory mediators in dengue virus infection in children: interleukin-6 and its relation to C-reactive protein and secretory phospholipase A2. Am J Trop Med Hyg. 65:70–75. 2001.PubMed/NCBI

19 

Dalrymple NA and Mackow ER: Endothelial cells elicit immune-enhancing responses to dengue virus infection. J Virol. 86:6408–6415. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Esteller M: Non-coding RNAs in human disease. Nat Rev Genet. 12:861–874. 2011. View Article : Google Scholar

21 

Guo H, Ingolia NT, Weissman JS and Bartel DP: Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature. 466:835–840. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Kong Y and Han JH: MicroRNA: biological and computational perspective. Genomics Proteomics Bioinformatics. 3:62–72. 2005.PubMed/NCBI

23 

Heiss BL, Maximova OA and Pletnev AG: Insertion of microRNA targets into the flavivirus genome alters its highly neurovirulent phenotype. J Virol. 85:1464–1472. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Lee TC, Lin YL, Liao JT, et al: Utilizing liver-specific microRNA-122 to modulate replication of dengue virus replicon. Biochem Biophys Res Commun. 396:596–601. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Pham AM, Langlois RA and TenOever BR: Replication in cells of hematopoietic origin is necessary for Dengue virus dissemination. PLoS Pathog. 8:e10024652012. View Article : Google Scholar : PubMed/NCBI

26 

Tolfvenstam T, Lindblom A, Schreiber MJ, et al: Characterization of early host responses in adults with dengue disease. BMC Infect Dis. 11:2092011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Qi Y, Li Y, Zhang L and Huang J: microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells. Mol Med Rep 7: 791-798, 2013.
APA
Qi, Y., Li, Y., Zhang, L., & Huang, J. (2013). microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells. Molecular Medicine Reports, 7, 791-798. https://doi.org/10.3892/mmr.2013.1288
MLA
Qi, Y., Li, Y., Zhang, L., Huang, J."microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells". Molecular Medicine Reports 7.3 (2013): 791-798.
Chicago
Qi, Y., Li, Y., Zhang, L., Huang, J."microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells". Molecular Medicine Reports 7, no. 3 (2013): 791-798. https://doi.org/10.3892/mmr.2013.1288
Copy and paste a formatted citation
x
Spandidos Publications style
Qi Y, Li Y, Zhang L and Huang J: microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells. Mol Med Rep 7: 791-798, 2013.
APA
Qi, Y., Li, Y., Zhang, L., & Huang, J. (2013). microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells. Molecular Medicine Reports, 7, 791-798. https://doi.org/10.3892/mmr.2013.1288
MLA
Qi, Y., Li, Y., Zhang, L., Huang, J."microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells". Molecular Medicine Reports 7.3 (2013): 791-798.
Chicago
Qi, Y., Li, Y., Zhang, L., Huang, J."microRNA expression profiling and bioinformatic analysis of dengue virus‑infected peripheral blood mononuclear cells". Molecular Medicine Reports 7, no. 3 (2013): 791-798. https://doi.org/10.3892/mmr.2013.1288
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team