Tanshinone IIA inhibits the growth of pancreatic cancer BxPC‑3 cells by decreasing protein expression of TCTP, MCL‑1 and Bcl‑xL

Huang,Cheng‑Yen; Chiu,Tsung‑Lang; Kuo,Shou‑Jen; Chien,Su‑Yu; Chen,Dar‑Ren; Su,Chin‑Cheng

doi:10.3892/mmr.2013.1290

March 2013 Volume 7 Issue 3

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March 2013 Volume 7 Issue 3

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Article

Tanshinone IIA inhibits the growth of pancreatic cancer BxPC‑3 cells by decreasing protein expression of TCTP, MCL‑1 and Bcl‑xL

Authors:
- Cheng‑Yen Huang
- Tsung‑Lang Chiu
- Shou‑Jen Kuo
- Su‑Yu Chien
- Dar‑Ren Chen
- Chin‑Cheng Su
View Affiliations / Copyright

Affiliations: Department of Surgery, Changhua Christian Hospital, Changhua 500‑06, Taiwan, R.O.C. , Tzu‑Chi University, Buddhist Tzu‑Chi General Hospital, Hualien 970, Taiwan, R.O.C., Department of Pharmacy, Changhua Christian Hospital, Changhua 500‑06, Taiwan, R.O.C.
Pages: 1045-1049
|
Published online on: January 25, 2013

https://doi.org/10.3892/mmr.2013.1290
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Abstract

Pancreatic cancer remains a challenging disease worldwide. Tanshinone IIA (Tan‑IIA) is one of the active constituents of Danshen (Radix Salviae miltiorrhizae). Tan‑IIA has been hypothesized to inhibit numerous human cancer cells by various molecular mechanisms. However, the efficacy and molecular mechanism of Tan‑IIA action in pancreatic cancer has not been well studied. In the present study, the cytotoxicity of Tan‑IIA in human pancreatic cancer BxPC‑3 cells was evaluated by MTT assay. Cell cycle analysis of BxPC‑3 cells treated with Tan‑IIA was performed by flow cytometry (FACS). Protein expression levels of TCTP, Mcl‑1, Bcl‑xL, Bax and Caspase‑3 in BxPC‑3 cells were measured by western blot analysis. The results revealed that Tan‑IIA inhibited BxPC‑3 cells in a time‑ and dose‑dependent manner. FACS analysis demonstrated that Tan‑IIA increases the rate of sub‑G1 phase. BxPC‑3 cells treated with Tan‑IIA were identified to upregulate protein expression of Bax and Caspase‑3 and downregulate expression of TCTP, Mcl‑1 and Bcl‑xL. These results indicate that Tan‑IIA may inhibit BxPC‑3 human pancreatic cancer cells through the induction of apoptosis by decreasing protein expression of TCTP, Mcl‑1 and Bcl‑xL and increasing Bax expression in vitro. The chemotherapeutic potential of Tan‑IIA for human pancreatic cancer warrants further study.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Tanshinone IIA inhibits the growth of pancreatic cancer BxPC‑3 cells by decreasing protein expression of TCTP, MCL‑1 and Bcl‑xL

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