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Article

Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells

  • Authors:
    • Jia-Le Song
    • Xin Zhao
    • Qiang Wang
    • Ting Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Research Center for Nutrition and Food Safety, The Third Military Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing 400038, P.R. China
  • Pages: 1607-1612
    |
    Published online on: March 26, 2013
       https://doi.org/10.3892/mmr.2013.1396
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Abstract

Reactive oxygen species (ROS)-induced pancreatic β cell death affects insulin secretion and is important in the pathogenesis of diabetes. Lagerstroemia speciosa, a traditional folk medicine, has been used for t he prevention and treatment of diabetes. However, whether Lagerstroemia speciosa has a cytoprotective effect on pancreatic β cells remains to be elucidated. The present study aimed to investigate the cytoprotective effects of hot water extracts from Lagerstroemia speciosa leaves (LWE) on 3-morpholinosydnonimine (SIN-1)-induced oxidative damage in Syrian hamster pancreatic insulinoma HIT-T15 cells. The HIT-T15 cells were first treated with SIN-1 (50 µM) for 24 h and then co-incubated with LWE for 48 h. SIN-1 significantly decreased HIT-T15 cell viability (P<0.05); however, LWE did not exert a significant cytotoxic effect and increased the viability of HIT-T15 cells in a dose‑dependent manner. To further investigate the protective effects of LWE on SIN-1‑induced oxidative stress in HIT-T15 cells, the cellular levels of ROS, lipid peroxidation and endogenous antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px), were determined. LWE decreased the intracellular levels of ROS and lipid peroxidation, and increased the activities of antioxidant enzymes. These results suggest that LWE has a cytoprotective effect against SIN-1‑induced oxidative stress in HIT-T15 cells through the inhibition of lipid peroxidation, a decrease in ROS levels and an increase in antioxidant enzyme activity. In addition, LWE increased insulin secretion in SIN-1-treated HIT-T15 cells. Our results suggested that LWE were effective in the treatment of diabetes. Further studies are required to study the anti-diabetic molecular mechanism in a cell model.
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Copy and paste a formatted citation
Spandidos Publications style
Song J, Zhao X, Wang Q and Zhang T: Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells. Mol Med Rep 7: 1607-1612, 2013.
APA
Song, J., Zhao, X., Wang, Q., & Zhang, T. (2013). Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells. Molecular Medicine Reports, 7, 1607-1612. https://doi.org/10.3892/mmr.2013.1396
MLA
Song, J., Zhao, X., Wang, Q., Zhang, T."Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells". Molecular Medicine Reports 7.5 (2013): 1607-1612.
Chicago
Song, J., Zhao, X., Wang, Q., Zhang, T."Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells". Molecular Medicine Reports 7, no. 5 (2013): 1607-1612. https://doi.org/10.3892/mmr.2013.1396
Copy and paste a formatted citation
x
Spandidos Publications style
Song J, Zhao X, Wang Q and Zhang T: Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells. Mol Med Rep 7: 1607-1612, 2013.
APA
Song, J., Zhao, X., Wang, Q., & Zhang, T. (2013). Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells. Molecular Medicine Reports, 7, 1607-1612. https://doi.org/10.3892/mmr.2013.1396
MLA
Song, J., Zhao, X., Wang, Q., Zhang, T."Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells". Molecular Medicine Reports 7.5 (2013): 1607-1612.
Chicago
Song, J., Zhao, X., Wang, Q., Zhang, T."Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells". Molecular Medicine Reports 7, no. 5 (2013): 1607-1612. https://doi.org/10.3892/mmr.2013.1396
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