Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells

Song,Jia-Le; Zhao,Xin; Wang,Qiang; Zhang,Ting

doi:10.3892/mmr.2013.1396

Protective effects of Lagerstroemia speciosa on 3-morpholinosydnonimine (SIN-1)-induced oxidative stress in HIT-T15 pancreatic β cells

Authors:
- Jia-Le Song
- Xin Zhao
- Qiang Wang
- Ting Zhang
View Affiliations

Affiliations: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Research Center for Nutrition and Food Safety, The Third Military Medical University, Chongqing Key Laboratory of Nutrition and Food Safety, Chongqing 400038, P.R. China
Published online on: March 26, 2013 https://doi.org/10.3892/mmr.2013.1396
Pages: 1607-1612

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Reactive oxygen species (ROS)-induced pancreatic β cell death affects insulin secretion and is important in the pathogenesis of diabetes. Lagerstroemia speciosa, a traditional folk medicine, has been used for t he prevention and treatment of diabetes. However, whether Lagerstroemia speciosa has a cytoprotective effect on pancreatic β cells remains to be elucidated. The present study aimed to investigate the cytoprotective effects of hot water extracts from Lagerstroemia speciosa leaves (LWE) on 3-morpholinosydnonimine (SIN-1)-induced oxidative damage in Syrian hamster pancreatic insulinoma HIT-T15 cells. The HIT-T15 cells were first treated with SIN-1 (50 µM) for 24 h and then co-incubated with LWE for 48 h. SIN-1 significantly decreased HIT-T15 cell viability (P<0.05); however, LWE did not exert a significant cytotoxic effect and increased the viability of HIT-T15 cells in a dose‑dependent manner. To further investigate the protective effects of LWE on SIN-1‑induced oxidative stress in HIT-T15 cells, the cellular levels of ROS, lipid peroxidation and endogenous antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px), were determined. LWE decreased the intracellular levels of ROS and lipid peroxidation, and increased the activities of antioxidant enzymes. These results suggest that LWE has a cytoprotective effect against SIN-1‑induced oxidative stress in HIT-T15 cells through the inhibition of lipid peroxidation, a decrease in ROS levels and an increase in antioxidant enzyme activity. In addition, LWE increased insulin secretion in SIN-1-treated HIT-T15 cells. Our results suggested that LWE were effective in the treatment of diabetes. Further studies are required to study the anti-diabetic molecular mechanism in a cell model.

View Figures

View References

1	Halliwell B: Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life. Plant Physiol. 141:312–22. 2006. View Article : Google Scholar : PubMed/NCBI
2	Evans JL, Goldfine ID, Maddux BA and Grodsky GM: Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction? Diabetes. 52:1–8. 2003. View Article : Google Scholar : PubMed/NCBI
3	Rahimi R, Nikfar S, Larijani B and Abdollahi M: A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother. 59:365–373. 2005. View Article : Google Scholar : PubMed/NCBI
4	Robertson RP and Harmon JS: Diabetes, glucose toxicity, and oxidative stress: a case of double jeopardy for the pancreatic islet beta cell. Free Radic Biol Med. 41:177–184. 2006. View Article : Google Scholar : PubMed/NCBI
5	Bell DS: Do sulfonylurea drugs increase the risk of cardiac events? CMAJ. 174:185–186. 2006. View Article : Google Scholar : PubMed/NCBI
6	Home PD, Pocock SJ, Beck-Nielsen H, Gomis R, Hanefeld M, Jones NP, Komajda M and McMurray JJ; RECORD Study Group. Rosiglitazone evaluated for cardiovascular outcomes - an interim analysis. N Engl J Med. 357:28–38. 2007. View Article : Google Scholar : PubMed/NCBI
7	Psaty BM and Furberg CD: The record on rosiglitazone and the risk of myocardial infarction. N Engl J Med. 357:67–69. 2007. View Article : Google Scholar : PubMed/NCBI
8	Nathan DM: Rosiglitazone and cardiotoxicity - weighing the evidence. N Engl J Med. 357:64–66. 2007. View Article : Google Scholar : PubMed/NCBI
9	Bailey CJ and Day C: Traditional plant medicines as treatments for diabetes. Diabetes Care. 12:553–564. 1989. View Article : Google Scholar : PubMed/NCBI
10	Garcia F: Distribution and deterioration of insulin-like principle in Lagerstroemia speciosa (banaba). Acta Med Philippina. 3:99–104. 1941.
11	Klein G, Kim J, Himmeldirk K, Cao Y and Chen X: Antidiabetes and anti-obesity activity of Lagerstroemia speciosa. Evid Based Complement Alternat Med. 4:401–407. 2007. View Article : Google Scholar
12	Mishra Y, Khan MSY, Zafar R and Agarwal SS: Hypoglycemic activity of leaves of Lagerstroemia speciosa (L) Pers. Indian J Pharmacol. 22:174–176. 1990.
13	Priya TT, Sabu MC and Jolly CI: Free radical scavenging and anti-inflammatory properties of Lagerstroemia speciosa (L). Inflammopharmacology. 16:182–187. 2008. View Article : Google Scholar : PubMed/NCBI
14	Khan MT, Lampronti I, Martello D, Bianchi N, Jabbar S, Choudhuri MS, Datta BK and Gambari R: Identification of pyrogallol as an antiproliferative compound present in extracts from the medicinal plant Emblica officinalis: effects on in vitro cell growth of human tumor cell lines. Int J Oncol. 21:187–192. 2002.PubMed/NCBI
15	Suzuki Y, Unno T, Ushitani M, Hayashi K and Kakuda T: Antiobesity activity of extracts from Lagerstroemia speciosa L. leaves on female KK-Ay mice. J Nutr Sci Vitaminol (Tokyo). 45:791–795. 1999. View Article : Google Scholar
16	Kakuda T, Sakane I, Takihara T, Ozaki Y, Takeuchi H and Kuroyanagi M: Hypoglycemic effect of extracts from Lagerstroemia speciosa L. leaves in genetically diabetic KK-AY mice. Biosci Biotechnol Biochem. 60:204–208. 1996.
17	Liu F, Kim J, Li Y, Liu X, Li J and Chen X: An extract of Lagerstroemia speciosa L. has insulin-like glucose uptake-stimulatory and adipocyte differentiation-inhibitory activities in 3T3-L1 cells. J Nutr. 131:2242–2247. 2001.PubMed/NCBI
18	Liu X, Kim JK, Li Y, Li J, Liu F and Chen X: Tannic acid stimulates glucose transport and inhibits adipocyte differentiation in 3T3-L1 cells. J Nutr. 135:165–171. 2005.PubMed/NCBI
19	Liu J, Sun H, Duan W, Mu D and Zhang L: Maslinic acid reduces blood glucose in KK-Ay mice. Biol Pharm Bull. 30:2075–2078. 2007. View Article : Google Scholar : PubMed/NCBI
20	Fraga CG, Leibovitz BE and Tappel AL: Lipid peroxidation measured as thiobarbituric acid-reactive substances in tissue slices: characterization and comparison with homogenates and microsomes. Free Radic Biol Med. 4:155–161. 1988. View Article : Google Scholar
21	Nelson DP and Kiesow LA: Enthalpy of decomposition of hydrogen peroxide by catalase at 25 degrees C (with molar extinction coefficients of H₂O₂ solutions in the UV). Anal Biochem. 49:474–478. 1972. View Article : Google Scholar : PubMed/NCBI
22	Marklund S and Marklund G: Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 47:469–474. 1974. View Article : Google Scholar : PubMed/NCBI
23	Hafeman DG, Sunde RA and Hoekstra WG: Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J Nutr. 104:580–587. 1974.PubMed/NCBI
24	Robertson RP, Harmon J, Tran PO, Tanaka Y and Takahashi H: Glucose toxicity in beta-cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes. 52:581–587. 2003. View Article : Google Scholar : PubMed/NCBI
25	Cheng Q, Law PK, de Gasparo M and Leung PS: Combination of the dipeptidyl peptidase IV inhibitor LAF237 [(S)-1-[(3-hydroxy-1-adamantyl)ammo]acetyl-2-cyanopyrrolidine] with the angiotensin II type 1 receptor antagonist valsartan [N-(1-oxopentyl)-N-[[2′-(1H-tetrazol-5-yl)-[1,1′-biphenyl]-4-yl]methyl]-L-valine] enhances pancreatic islet morphology and function in a mouse model of type 2 diabetes. J Pharmacol Exp Ther. 327:683–691. 2008.
26	Lenzen S: Oxidative stress: the vulnerable beta-cell. Biochem Soc Trans. 36:343–347. 2008. View Article : Google Scholar : PubMed/NCBI
27	Zhang H, Ollinger K and Brunk U: Insulinoma cells in culture show pronounced sensitivity to alloxan-induced oxidative stress. Diabetologia. 38:635–641. 1995. View Article : Google Scholar : PubMed/NCBI
28	Moriscot C, Pattou F, Kerr-Conte J, Richard MJ, Lemarchand P and Benhamou PY: Contribution of adenoviral-mediated superoxide dismutase gene transfer to the reduction in nitric oxide-induced cytotoxicity on human islets and INS-1 insulin-secreting cells. Diabetologia. 43:625–631. 2000. View Article : Google Scholar
29	Kubisch HM, Wang J, Bray TM and Phillips JP: Targeted overexpression of Cu/Zn superoxide dismutase protects pancreatic beta-cells against oxidative stress. Diabetes. 46:1563–1566. 1997. View Article : Google Scholar : PubMed/NCBI
30	Kubisch HM, Wang J, Luche R, Carlson E, Bray TM, Epstein CJ and Phillips JP: Transgenic copper/zinc superoxide dismutase modulates susceptibility to type I diabetes. Proc Natl Acad Sci USA. 91:9956–9959. 1994. View Article : Google Scholar : PubMed/NCBI
31	Xu B, Moritz JT and Epstein PN: Overexpression of catalase provides partial protection to transgenic mouse beta cells. Free Radic Biol Med. 27:830–837. 1999. View Article : Google Scholar : PubMed/NCBI
32	Amstad P, Moret R and Cerutti P: Glutathione peroxidase compensates for the hypersensitivity of Cu,Zn-superoxide dismutase overproducers to oxidant stress. J Biol Chem. 269:1606–1609. 1994.PubMed/NCBI
33	Lortz S and Tiedge M: Sequential inactivation of reactive oxygen species by combined overexpression of SOD isoforms and catalase in insulin-producing cells. Free Radic Biol Med. 34:683–688. 2003. View Article : Google Scholar : PubMed/NCBI
34	Lepore DA, Shinkel TA, Fisicaro N, Mysore TB, Johnson LE, d’Apice AJ and Cowan PJ: Enhanced expression of glutathione peroxidase protects islet beta cells from hypoxia-reoxygenation. Xenotransplantation. 11:53–59. 2004. View Article : Google Scholar : PubMed/NCBI
35	Mysore TB, Shinkel TA, Collins J, Salvaris EJ, Fisicaro N, Murray-Segal LJ, Johnson LE, Lepore DA, Walters SN, Stokes R, Chandra AP, O’Connell PJ, d’Apice AJ and Cowan PJ: Overexpression of glutathione peroxidase with two isoforms of superoxide dismutase protects mouse islets from oxidative injury and improves islet graft function. Diabetes. 54:2109–2116. 2005. View Article : Google Scholar : PubMed/NCBI