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Article

Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells

  • Authors:
    • Jin-Ming Zhu
    • Pei-Wu Yu
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    Affiliations: Department of General Surgery, Jinan Military General Hospital, Jinan, Shandong 250031, P.R. China, Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 425-433
    |
    Published online on: June 7, 2013
       https://doi.org/10.3892/mmr.2013.1513
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Abstract

T‑cell lymphoma invasion and metastasis-inducing factor 1 (Tiam‑1) is an important member of the diffuse B-cell lymphoma (Dbl) oncogene family. In a previous study, the overexpression of Tiam‑1 protein was identified by immunohistochemistry in human gastric cancer tissues, indicating that Tiam‑1 may represent a candidate biomarker of the invasive and metastatic capacity of gastric cancer and for patient prognosis. In the present study, in vitro adhesion selection was used to separate two subpopulations with high (MH) or low (ML) invasive and metastatic potential from the MKN‑45 human gastric cancer cell line (M0). A positive correlation was observed between Tiam‑l mRNA and protein expression levels and the invasive capacity of the cells using RT‑PCR and quantitative cellular‑ELISA, respectively. To determine the mechanism by which Tiam‑1 affects the invasive capacities of gastric cancer cells, Tiam‑1 expression was downregulated in the MH subclone by liposomal transfection of antisense oligodeoxynucleotides (ASODNs). Following 48 h of treatment with ASODNs (0.43 µM), Tiam‑1 mRNA transcription and protein expression levels in MH cells was decreased by 80 and 24%, respectively, compared with untreated controls. In addition, the in vitro invasive potential of MH cells was suppressed by 60%. Morphological and ultrastructural observations also demonstrated that ASODN‑treated MH cells exhibited a smooth surface with markedly reduced filopodia and microspikes, which resembled M0 and ML cells. In addition, cytoskeletal distribution was markedly altered from disordered to regular with reduced long filament‑like structures, projections, pseudopodia on the cell surface and decreased actin bodies in the cytoplasm. Results of the current study indicate that the overexpression of Tiam‑1 contributes to the invasive phenotypes of gastric cancer cells. These observations are likely to provide an improved insight into the biological mechanisms of Tiam‑1 and promote the development of novel treatment strategies in gastric cancer.
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View References

1 

Mahar AL, McLeod RS, Kiss A, Paszat L and Coburn NG: A systematic review of the effect of institution and surgeon factors on surgical outcomes for gastric cancer. J Am Coll Surg. 214:860–868. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Lee JH, Kim KM, Cheong JH and Noh SH: Current management and future strategies of gastric cancer. Yonsei Med J. 53:248–257. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Lin LL, Huang HC and Juan HF: Discovery of biomarkers for gastric cancer: a proteomics approach. J Proteomics. 75:3081–3097. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Habets GG, Scholtes EH, Zuydgeest D, van der Kammen RA, Stam JC, Berns A and Collard JG: Identification of an invasion-inducing gene, Tiam-1, that encodes a protein with homology to GDP-GTP exchangers for Rho-like proteins. Cell. 77:537–549. 1994. View Article : Google Scholar : PubMed/NCBI

5 

Habets GG, van der Kammen RA, Jenkins NA, Gilbert DJ, Copeland NG, Hagemeijer A and Collard JG: The invasion-inducing TIAM1 gene maps to human chromosome band 21q22 and mouse chromosome 16. Cytogenet Cell Genet. 70:48–51. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Mertens AE, Roovers RC and Collard JG: Regulation of Tiam1-Rac signalling. FEBS Lett. 546:11–16. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Minard ME, Kim LS, Price JE and Gallick GE: The role of the guanine nucleotide exchange factor Tiam1 in cellular migration, invasion, adhesion and tumor progression. Breast Cancer Res Treat. 84:21–32. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Chen B, Ding Y, Liu F, et al: Tiam1, overexpressed in most malignancies, is a novel tumor biomarker. Mol Med Rep. 5:48–53. 2012.PubMed/NCBI

9 

Ding Y, Chen B, Wang S, et al: Overexpression of Tiam1 in hepatocellular carcinomas predicts poor prognosis of HCC patients. Int J Cancer. 124:653–658. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Minard ME, Ellis LM and Gallick GE: Tiam1 regulates cell adhesion, migration and apoptosis in colon tumor cells. Clin Exp Metastasis. 23:301–313. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Zhu JM, Yu PW and Zhao YL: Relationship between the expression of Tiam-1, Rac 1 and the pathobiological behavior of gastric cancer. Chin J Gen Surg. 14:168–172. 2005.

12 

Chen XR, Ren WP, Dong JF, Xiao SD and Sloane BF: Screening of gastric cancer cell sublines by adhesion method in vitro. Chin J Gastroenterol. 2:121–124. 2001.

13 

Yokozaki H: Molecular characteristics of eight gastric cancer cell lines established in Japan. Pathol Int. 50:767–777. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Li ZZ, Zhang L, Mao HT, Wang Y, Li DH and Gui SL: Effect of Tiam-1 antisense oligodeoxynucleotides (ASODNS) on antimetastasis of tumor. Chin J Cancer Biother. 50:767–777. 2000.

15 

Zhang XQ, Zhang R, Gu CH and Wang YM: Expression level of ICAM-1 on cultured cells measured quantitatively with cellular ELISA. Acta Acad Med Mil Tert. 23:117–118. 2001.

16 

Zhang JH, Ding YQ and Yang XJ: Introduction of a reforming staining method for cytoskeletal proteins. J Diag Pathol. 3:235–236. 1996.

17 

Albini A, Iwamoto Y, Kleinman HK, Martin GR, Aaronson SA, Kozlowski JM and McEwan RN: A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47:3239–3245. 1987.PubMed/NCBI

18 

Lleonart ME, Martin-Duque P, Sanchez-Prieto R, Moreno A and Ramon y Cajal S: Tumor heterogeneity: morphological, molecular and clinical implications. Histol Histopathol. 15:881–898. 2000.PubMed/NCBI

19 

Marian AJ: Molecular genetic studies of complex phenotypes. Transl Res. 159:64–79. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Prasun P, Pradhan M and Agarwal S: One gene, many phenotypes. J Postgrad Med. 53:257–261. 2007. View Article : Google Scholar

21 

Etienne MS and Hall A: Rho GTPases in cell biology. Nature. 420:629–635. 2002. View Article : Google Scholar

22 

Malliri A and Collard JG: Role of Rho-family proteins in cell adhesion and cancer. Curr Opin Cell Biol. 15:583–589. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Hirohashi S and Kanai Y: Cell adhesion system and human cancer morphogenesis. Cancer Sci. 94:575–581. 2003. View Article : Google Scholar : PubMed/NCBI

24 

Ben-Ze’ev A: The cytoskeleton in cancer cells. Biochim Biophys Acta. 780:197–212. 1985.

25 

Ehler E, van Leeuwen F, Collard JG and Salinas PC: Expression of Tiam-1 in the developing brain suggests a role for the Tiam-1-Rac signaling pathway in cell migration and neurite outgrowth. Mol Cell Neurosci. 9:1–12. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Okegawa T, Pong RC, Li Y and Hsieh JT: The role of cell adhesion molecule in cancer progression and its application in cancer therapy. Acta Biochim Pol. 51:445–457. 2004.PubMed/NCBI

27 

Marhaba R and Zoller M: CD44 in cancer progression: adhesion, migration and growth regulation. J Mol Histol. 35:211–231. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Ekblom P, Lonai P and Talts JF: Expression and biological role of laminin-1. Matrix Biol. 22:35–47. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Kim WH, Lee BL, Jun SH, Song SY and Kleinman HK: Expression of 32/67-kDa laminin receptor in laminin adhesion-selected human colon cancer cell lines. Br J Cancer. 77:15–20. 1998. View Article : Google Scholar : PubMed/NCBI

30 

Kim WH, Jun SH, Kibbey MC, Thompson EW and Kleinman HK: Expression of beta 1 integrin in laminin-adhesion-selected human colon cancer cell lines of varying tumorigenicity. Invasion Metastasis. 14:147–155. 1995.PubMed/NCBI

31 

Brábek J, Mierke CT, Rösel D, Veselý P and Fabry B: The role of the tissue microenvironment in the regulation of cancer cell motility and invasion. Cell Commun Signal. 8:222010.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhu J and Yu P: Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells. Mol Med Rep 8: 425-433, 2013.
APA
Zhu, J., & Yu, P. (2013). Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells. Molecular Medicine Reports, 8, 425-433. https://doi.org/10.3892/mmr.2013.1513
MLA
Zhu, J., Yu, P."Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells". Molecular Medicine Reports 8.2 (2013): 425-433.
Chicago
Zhu, J., Yu, P."Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells". Molecular Medicine Reports 8, no. 2 (2013): 425-433. https://doi.org/10.3892/mmr.2013.1513
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J and Yu P: Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells. Mol Med Rep 8: 425-433, 2013.
APA
Zhu, J., & Yu, P. (2013). Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells. Molecular Medicine Reports, 8, 425-433. https://doi.org/10.3892/mmr.2013.1513
MLA
Zhu, J., Yu, P."Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells". Molecular Medicine Reports 8.2 (2013): 425-433.
Chicago
Zhu, J., Yu, P."Downregulation of T‑cell lymphoma invasion and metastasis‑inducing factor 1 induces cytoskeletal rearrangement and inhibits the invasive capacity of gastric cancer cells". Molecular Medicine Reports 8, no. 2 (2013): 425-433. https://doi.org/10.3892/mmr.2013.1513
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