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Article

Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis

  • Authors:
    • Yan Zhou
    • Yuanhong Liang
    • Jie Wei
    • Jing Chen
    • Qizhu Tang
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Cardiology, Guangdong General Hospital, Guangdong Cardiovascular Institute, Guangzhou, Guangdong 510080, P.R. China, Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 493-498
    |
    Published online on: June 21, 2013
       https://doi.org/10.3892/mmr.2013.1539
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Abstract

Aldosterone-induced myocyte apoptosis is an important component of cardiovascular disease. While the p38 mitogen-activated protein kinase (p38 MAPK) pathway has been shown to be crucial in myocyte apoptosis, whether aldosterone induces myocyte apoptosis through this pathway remains unclear. In the present study, three individual strands of p38 MAPK short hairpin RNA (ShRNA), delivered by lentiviral vectors (PGLV), were constructed and used to explore the role of p38 MAPK pathway activation in aldosterone-mediated myocyte apoptosis in cultured myocytes and normotensive rats. Aldosterone stimulation increased myocyte apoptosis, caspase-3 expression levels and p38 MAPK mRNA and protein expression levels in vitro and in vivo. PGLV-ShRNA3 transduction decreased aldosterone-mediated myocyte apoptosis and p38 MAPK mRNA and protein expression levels in vitro (all P<0.01). PGLV-ShRNA3 transduction significantly decreased aldosterone-mediated myocyte apoptosis, p38 MAPK mRNA and protein expression levels in normotensive rats (P<0.01, P<0.01 and P<0.05, respectively). Results from the present study suggest that aldosterone directly induces myocyte apoptosis through the p38 MAPK pathway and the gene silencing of p38 MAPK may protect cardiac myocytes from aldosterone-mediated apoptosis.
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1 

Fraccarollo D, Berger S, Galuppo P, et al: Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse remodeling after myocardial infarction. Circulation. 123:400–408. 2011. View Article : Google Scholar

2 

Messaoudi S, Azibani F, Delcayre C and Jaisser F: Aldosterone, mineralocorticoid receptor, and heart failure. Mol Cell Endocrinol. 350:266–272. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Güder G, Bauersachs J, Frantz S, et al: Complementary and incremental mortality risk prediction by cortisol and aldosterone in chronic heart failure. Circulation. 115:1754–1761. 2007.PubMed/NCBI

4 

Iraqi W, Rossignol P, Angioi M, et al: Extracellular cardiac matrix biomarkers in patients with acute myocardial infarction complicated by left ventricular dysfunction and heart failure: insights from the Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study (EPHESUS) study. Circulation. 119:2471–2479. 2009.

5 

Rossignol P, Ménard J, Fay R, Gustafsson F, Pitt B and Zannad F: Eplerenone survival benefits in heart failure patients post-myocardial infarction are independent from its diuretic and potassium-sparing effects. Insights from an EPHESUS (Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study) substudy. J Am Coll Cardiol. 58:1958–1966. 2011.

6 

Zannad F, McMurray JJV, Krum H, et al; EMPHASIS-HF Study Group. Eplerenone in patients with systolic heart failure and mild symptoms. N Engl J Med. 364:11–21. 2011. View Article : Google Scholar

7 

Kuster GM, Kotlyar E, Rude MK, et al: Mineralocorticoid receptor inhibition ameliorates the transition to myocardial failure and decreases oxidative stress and inflammation in mice with chronic pressure overload. Circulation. 111:420–427. 2005. View Article : Google Scholar

8 

Usher MG, Duan SZ, Ivaschenko CY, et al: Myeloid mineralocorticoid receptor controls macrophage polarization and cardiovascular hypertrophy and remodeling in mice. J Clin Invest. 120:3350–3364. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Sun HY, Wang NP, Halkos M, et al: Postconditioning attenuates cardiomyocyte apoptosis via inhibition of JNK and p38 mitogen-activated protein kinase signaling pathways. Apoptosis. 11:1583–1593. 2006. View Article : Google Scholar

10 

Kinoshita H, Kuwahara K, Takano M, et al: T-type Ca2+ channel blockade prevents sudden death in mice with heart failure. Circulation. 120:743–752. 2009.

11 

Mano A, Tatsumi T, Shiraishi J, et al: Aldosterone directly induces myocyte apoptosis through calcineurin-dependent pathways. Circulation. 110:317–323. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Ferron L, Ruchon Y, Renaud JF and Capuano V: T-type Ca2+ signalling regulates aldosterone-induced CREB activation and cell death through PP2A activation in neonatal cardiomyocytes. Cardiovasc Res. 90:105–112. 2011.

13 

Dhingra S, Sharma AK, Singla DK and Singal PK: p38 and ERK1/2 MAPKs mediate the interplay of TNF-alpha and IL-10 in regulating oxidative stress and cardiac myocyte apoptosis. Am J Physiol Heart Circ Physiol. 293:H3524–H3531. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Yeh CC, Li H, Malhotra D, et al: Distinctive ERK and p38 signaling in remote and infarcted myocardium during post-MI remodeling in the mouse. J Cell Biochem. 109:1185–1191. 2010.PubMed/NCBI

15 

Cheriyan J, Webb AJ, Sarov-Blat L, et al: Inhibition of p38 mitogen-activated protein kinase improves nitric oxide-mediated vasodilatation and reduces inflammation in hypercholesterolemia. Circulation. 123:515–523. 2011. View Article : Google Scholar

16 

Ma XL, Kumar S, Gao F, et al: Inhibition of p38 mitogen-activated protein kinase decreases cardiomyocyte apoptosis and improves cardiac function after myocardial ischemia and reperfusion. Circulation. 99:1685–1691. 1999. View Article : Google Scholar

17 

See F, Thomas W, Way K, et al: p38 mitogen-activated protein kinase inhibition improves cardiac function and attenuates left ventricular remodeling following myocardial infarction in the rat. J Am Coll Cardiol. 44:1679–1689. 2004. View Article : Google Scholar

18 

De Silva DS, Wilson RM, Hutchinson C, et al: Fenofibrate inhibits aldosterone-induced apoptosis in adult rat ventricular myocytes via stress-activated kinase-dependent mechanisms. Am J Physiol Heart Circ Physiol. 296:H1983–H1993. 2009.PubMed/NCBI

19 

Thomas WG, Brandenburger Y, Autelitano DJ, Pham T, Qian H and Hannan RD: Adenoviral-directed expression of the type 1A angiotensin receptor promotes cardiomyocyte hypertrophy via transactivation of the epidermal growth factor receptor. Circ Res. 90:135–142. 2002. View Article : Google Scholar

20 

Coleman JE, Huentelman MJ, Kasparov S, et al: Efficient large-scale production and concentration of HIV-1-based lentiviral vectors for use in vivo. Physiol Genomics. 12:221–228. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Casciola-Rosen L, Nicholson DW, Chong T, et al: Apopain/CPP32 cleaves proteins that are essential for cellular repair: a fundamental principle of apoptotic death. J Exp Med. 183:1957–1964. 1996. View Article : Google Scholar : PubMed/NCBI

22 

Li Z, Ma JY, Kerr I, Chakravarty S, Dugar S, Schreiner G and Protter AA: Selective inhibition of p38alpha MAPK improves cardiac function and reduces myocardial apoptosis in rat model of myocardial injury. Am J Physiol Heart Circ Physiol. 291:H1972–H1977. 2006. View Article : Google Scholar : PubMed/NCBI

23 

De Angelis N, Fiordaliso F, Latini R, et al: Appraisal of the role of angiotensin II and aldosterone in ventricular myocyte apoptosis in adult normotensive rat. J Mol Cell Cardiol. 34:1655–1665. 2002.PubMed/NCBI

24 

Aouadi M, Tesz GJ, Nicoloro SM, et al: Orally delivered siRNA targeting macrophage Map4k4 suppresses systemic inflammation. Nature. 458:1180–1184. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Whitehead KA, Langer R and Anderson DG: Knocking down barriers: advances in siRNA delivery. Nat Rev Drug Discov. 8:129–138. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Liang Y, Wei J, Chen J and Tang Q: Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis. Mol Med Rep 8: 493-498, 2013.
APA
Zhou, Y., Liang, Y., Wei, J., Chen, J., & Tang, Q. (2013). Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis. Molecular Medicine Reports, 8, 493-498. https://doi.org/10.3892/mmr.2013.1539
MLA
Zhou, Y., Liang, Y., Wei, J., Chen, J., Tang, Q."Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis". Molecular Medicine Reports 8.2 (2013): 493-498.
Chicago
Zhou, Y., Liang, Y., Wei, J., Chen, J., Tang, Q."Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis". Molecular Medicine Reports 8, no. 2 (2013): 493-498. https://doi.org/10.3892/mmr.2013.1539
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Liang Y, Wei J, Chen J and Tang Q: Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis. Mol Med Rep 8: 493-498, 2013.
APA
Zhou, Y., Liang, Y., Wei, J., Chen, J., & Tang, Q. (2013). Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis. Molecular Medicine Reports, 8, 493-498. https://doi.org/10.3892/mmr.2013.1539
MLA
Zhou, Y., Liang, Y., Wei, J., Chen, J., Tang, Q."Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis". Molecular Medicine Reports 8.2 (2013): 493-498.
Chicago
Zhou, Y., Liang, Y., Wei, J., Chen, J., Tang, Q."Lentiviral-mediated p38 MAPK RNAi attenuates aldosterone-induced myocyte apoptosis". Molecular Medicine Reports 8, no. 2 (2013): 493-498. https://doi.org/10.3892/mmr.2013.1539
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