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Molecular Medicine Reports
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August 2013 Volume 8 Issue 2

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Article

Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation

  • Authors:
    • Changqing Zuo
    • Zonggui Wang
    • Hanyun Lu
    • Zhong Dai
    • Xinguang Liu
    • Liao Cui
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China, Department of Biochemistry and Molecular Biology, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China
  • Pages: 463-467
    |
    Published online on: June 21, 2013
       https://doi.org/10.3892/mmr.2013.1540
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Abstract

Protein‑coding genes and small non‑coding microRNAs involved in the guidance of differentiation in mesenchymal stem cells (MSCs) into osteoblasts have been extensively investigated in previous studies. However, long non‑coding RNAs (lncRNAs), which account for a large proportion of the genomic sequences in numerous species, have not yet been reported. In the present study, the lncRNA expression profile was analyzed using the Arraystar lncRNA array in C3H10T1/2 MSCs undergoing early osteoblast differentiation and 116 differentially expressed lncRNAs were identified between BMP‑2 treated and untreated groups. Among these lncRNAs, 59 were upregulated and 57 were downregulated in BMP‑2 treated groups. In addition, 24 cooperatively differentially expressed lncRNAs and nearby mRNA pairs were found. For example, mouselincRNA0231 and its nearby gene, EGFR, were downregulated, while lncRNA NR_027652 and its nearby gene, DLK1, were upregulated. These observations may be part of the regulatory mechanisms of lncRNAs in the control of osteoblast differentiaton. In conclusion, results of the present study indicate that lncRNA expression profiles are significantly altered in C3H10T1/2 undergoing early osteoblast differentiation and these results may provide insight into the mechanisms responsible for osteoblast differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Zuo C, Wang Z, Lu H, Dai Z, Liu X and Cui L: Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation. Mol Med Rep 8: 463-467, 2013.
APA
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., & Cui, L. (2013). Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation. Molecular Medicine Reports, 8, 463-467. https://doi.org/10.3892/mmr.2013.1540
MLA
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., Cui, L."Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation". Molecular Medicine Reports 8.2 (2013): 463-467.
Chicago
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., Cui, L."Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation". Molecular Medicine Reports 8, no. 2 (2013): 463-467. https://doi.org/10.3892/mmr.2013.1540
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo C, Wang Z, Lu H, Dai Z, Liu X and Cui L: Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation. Mol Med Rep 8: 463-467, 2013.
APA
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., & Cui, L. (2013). Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation. Molecular Medicine Reports, 8, 463-467. https://doi.org/10.3892/mmr.2013.1540
MLA
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., Cui, L."Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation". Molecular Medicine Reports 8.2 (2013): 463-467.
Chicago
Zuo, C., Wang, Z., Lu, H., Dai, Z., Liu, X., Cui, L."Expression profiling of lncRNAs in C3H10T1/2 mesenchymal stem cells undergoing early osteoblast differentiation". Molecular Medicine Reports 8, no. 2 (2013): 463-467. https://doi.org/10.3892/mmr.2013.1540
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