Staphylococcal enterotoxin B and α‑toxin induce the apoptosis of ECV304 cells via similar mechanisms

Yu,Feng‑Ling; Liu,Ting‑Ting; Zhu,Xiang; Yang,Weng‑Xuan; Zhang,Tao; Lin,Na; Liu,Yong; Liu,Cong‑Sen; Jiang,Jiu; Guan,Jun‑Chang

doi:10.3892/mmr.2013.1550

Staphylococcal enterotoxin B and α‑toxin induce the apoptosis of ECV304 cells via similar mechanisms

Authors:
- Feng‑Ling Yu
- Ting‑Ting Liu
- Xiang Zhu
- Weng‑Xuan Yang
- Tao Zhang
- Na Lin
- Yong Liu
- Cong‑Sen Liu
- Jiu Jiang
- Jun‑Chang Guan
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Affiliations: Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Microbiology, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China, Department of Biology, Drexel University College of Arts and Sciences, Philadelphia, PA 19104, USA
Published online on: June 25, 2013 https://doi.org/10.3892/mmr.2013.1550
Pages: 591-596

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Abstract

Staphylococcal enterotoxin B (SEB) and α‑toxin produced by Staphylococcus aureus (S. aureus) are important in the pathogenesis of diseases. In the present study, we investigated the effects of SEB and α‑toxin on ECV304 cells. It was identified that both SEB and α‑toxin were capable of inducing the apoptosis of ECV304 cells in a dose‑ and time‑dependent manner. In addition, SEB and α‑toxin were able to induce the expression of TNF‑α and the activation of caspase‑3 and ‑8 in the ECV304 cells. The inhibition of TNF‑α (with its neutralizing antibody) and caspase‑3 and ‑8 [with the corresponding inhibitory peptides; z-N-acetyl-Asp-Glu-Val-Asp-aminomethyl-coumarin (DEVD)-fluoromethyl ketone (FMK) for inhibition of caspase‑3 and z-N-acetyl-Ile-Glu-Thr-Asp (IETD)-FMK) for inhibition of caspase‑8] significantly decreased the rates of cell apoptosis induced by SEB and α‑toxin, but was not able to completely block the induced cell apoptosis. These data suggest that SEB and α‑toxin induce ECV304 cell apoptosis via a similar mechanism, which is partially mediated by the extrinsic death pathway involving TNF‑α and caspase‑8. These results provide insights into the synergistic pathogenicity of SEB and α‑toxin during S. aureus infection.

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