Immunocytochemistry of GLUT2, uptake of fluorescent desnitroso-streptozotocin analogs and phosphorylation of D-glucose in INS-1E cells

Virreira,Myrna; Popescu,Iuliana; Gillet,Celine; Zhang,Ying; Leshch,Yevgeniy; Thimm,Julian; Thiem,Joachim; Malaisse,Willy  J.; Sener,Abdullah

doi:10.3892/mmr.2013.1559

August 2013 Volume 8 Issue 2

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August 2013 Volume 8 Issue 2

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Article

Immunocytochemistry of GLUT2, uptake of fluorescent desnitroso-streptozotocin analogs and phosphorylation of D-glucose in INS-1E cells

Authors:
- Myrna Virreira
- Iuliana Popescu
- Celine Gillet
- Ying Zhang
- Yevgeniy Leshch
- Julian Thimm
- Joachim Thiem
- Willy J. Malaisse
- Abdullah Sener
View Affiliations / Copyright

Affiliations: Laboratory of Cell and Molecular Physiology, Université Libre de Bruxelles, Brussels B-1070, Belgium, Laboratory of Histology, Neuroanatomy and Neuropathology, Université Libre de Bruxelles, Brussels B-1070, Belgium, Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels B-1070, Belgium, Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels B-1070, Belgium, Department of Chemistry, University of Hamburg, Hamburg D-20146, Germany
Pages: 473-479
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Published online on: June 27, 2013

https://doi.org/10.3892/mmr.2013.1559
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Abstract

The non-invasive imaging of GLUT2-expressing cells remains a challenge. As streptozotocin, and similarly alloxan, may be transported into cells by GLUT2, the major aim of the present study was to assess the possible use of fluorescent desnitroso-streptozotocin analogs for in vitro labeling of GLUT2-expressing cells. INS-1E cells, human embryonic kidney (HEK) cells, rat isolated pancreatic islets, rat hepatic cells, rat exocrine pancreatic cells and tumoral insulin-producing BRIN-BD11 cells were incubated in the presence of two distinct fluorescent desnitroso-streptozotocin analogs, probes A and B. The immunocytochemistry of GLUT2 in INS-1E cells and the phosphorylation of D-glucose by INS-1E cell homogenates were also examined. The uptake of probes A and B (12.0 µM) by INS-1E cells yielded apparent intracellular concentrations approximately one order of magnitude higher than the extracellular concentration. The two probes differed from one another by the absolute values for their respective uptake and time course, but not so by the pattern of their concentration dependency. Comparable results were recorded in HEK cells, rat isolated pancreatic islets and hepatocytes. Vastly different findings were recorded, however, in rat exocrine pancreatic cells, which do not express GLUT2. Moreover, an unusual concentration dependency for the uptake of each probe was observed in tumoral BRIN-BD11 cells. It is proposed that suitable fluorescent desnitroso-streptozotocin analogs may be used to label GLUT2-expressing cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Immunocytochemistry of GLUT2, uptake of fluorescent desnitroso-streptozotocin analogs and phosphorylation of D-glucose in INS-1E cells

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