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Molecular Medicine Reports
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November 2013 Volume 8 Issue 5

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Article

Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study

  • Authors:
    • Lanhai Lü
    • Li Luo
    • Yinghong Lu
    • Lizhi Chen
    • Jie Xu
    • Kaihua Guo
  • View Affiliations / Copyright

    Affiliations: Guanghua School of Stomatology, Hospital of Stomatology, Institute of Stomatological Research, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 1359-1364
    |
    Published online on: September 2, 2013
       https://doi.org/10.3892/mmr.2013.1664
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Abstract

Progranulin (PGRN) is a neurotrophic factor that regulates neurite outgrowth and enhances neuronal survival. The association between PGRN and neural stem cell (NSC) differentiation may aid in elucidating the underlying pathogenesis and potential treatments for neurodegenerative diseases. To investigate the association between PGRN and NSCs and their lineages, primary NSCs were prepared from the subventricular zone of neonatal Sprague Dawley pups (age, 1 day) and cultured in the neural stem cell medium. After 7 days in culture, NSCs aggregated into neurospheres and were maintained in the differential medium for 7 days following three passages. In addition, PGRN expression in neurospheres and differentiated cells (neurons, astrocytes and oligodendrocytes) were analyzed by immunocytochemistry. The PGRN expression and localization was also investigated in the brain tissues of neonatal rats (age, 1 and 7 days) by double immunofluorescence staining. The data indicated that PGRN was highly expressed in NSCs and their differentiated cell lineages in vitro. The results also demonstrated that PGRN was predominantly expressed in neurons and microglia in vivo, and marginally in NSCs, astrocytes and oligodendrocytes. The present study suggested that PGRN may be involved in the regulation of NSC differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Lü L, Luo L, Lu Y, Chen L, Xu J and Guo K: Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study. Mol Med Rep 8: 1359-1364, 2013.
APA
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., & Guo, K. (2013). Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study. Molecular Medicine Reports, 8, 1359-1364. https://doi.org/10.3892/mmr.2013.1664
MLA
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., Guo, K."Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study". Molecular Medicine Reports 8.5 (2013): 1359-1364.
Chicago
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., Guo, K."Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study". Molecular Medicine Reports 8, no. 5 (2013): 1359-1364. https://doi.org/10.3892/mmr.2013.1664
Copy and paste a formatted citation
x
Spandidos Publications style
Lü L, Luo L, Lu Y, Chen L, Xu J and Guo K: Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study. Mol Med Rep 8: 1359-1364, 2013.
APA
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., & Guo, K. (2013). Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study. Molecular Medicine Reports, 8, 1359-1364. https://doi.org/10.3892/mmr.2013.1664
MLA
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., Guo, K."Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study". Molecular Medicine Reports 8.5 (2013): 1359-1364.
Chicago
Lü, L., Luo, L., Lu, Y., Chen, L., Xu, J., Guo, K."Progranulin expression in neural stem cells and their differentiated cell lineages: An immunocytochemical study". Molecular Medicine Reports 8, no. 5 (2013): 1359-1364. https://doi.org/10.3892/mmr.2013.1664
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