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Article

Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide

  • Authors:
    • Xue-Chao Lv
    • Liang-Jia Bi
    • Ying Jiang
    • Xiang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Dentistry, School of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Tooth Planting, School of Stomatology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
  • Pages: 1411-1415
    |
    Published online on: September 13, 2013
       https://doi.org/10.3892/mmr.2013.1677
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Abstract

Icariin (ICA), a flavanoid isolated from herbal Epimedium, has multiple biological activities. The present study investigated the effects of ICA on the proliferation and alkaline phosphatase (ALP) activity (an index for PDLC differentiation) of human periodontal ligament cells (hPDLCs) inhibited by lipopolysaccharide (LPS). hPDLCs were cultured in vitro and stimulated with various concentrations of ICA. The proliferation ability of hPDLCs was detected by an MTT assay. The activity of ALP was determined by the p‑Nitrophenyl phosphate method, and the expression of ALP was analyzed by reverse transcription polymerase chain reaction and western blot analysis. ICA exhibited a dose-dependent effect on the proliferation of hPDLCs in a suitable concentration range, from 10‑6 to 10‑8 mol/l, and with a mediate optimal concentration (10-6 mol/l). The alkaline phosphatase activity was markedly inhibited in 10 µg/ml LPS‑treated PDLCs and this inhibition was suppressed in the presence of icariin at a concentration of 10-6 mol/L following prolonged treatment (96 h). Therefore, this study provided insight into the use of ICA for periapical tissue regeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Lv X, Bi L, Jiang Y and Wang X: Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide. Mol Med Rep 8: 1411-1415, 2013.
APA
Lv, X., Bi, L., Jiang, Y., & Wang, X. (2013). Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide. Molecular Medicine Reports, 8, 1411-1415. https://doi.org/10.3892/mmr.2013.1677
MLA
Lv, X., Bi, L., Jiang, Y., Wang, X."Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide". Molecular Medicine Reports 8.5 (2013): 1411-1415.
Chicago
Lv, X., Bi, L., Jiang, Y., Wang, X."Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide". Molecular Medicine Reports 8, no. 5 (2013): 1411-1415. https://doi.org/10.3892/mmr.2013.1677
Copy and paste a formatted citation
x
Spandidos Publications style
Lv X, Bi L, Jiang Y and Wang X: Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide. Mol Med Rep 8: 1411-1415, 2013.
APA
Lv, X., Bi, L., Jiang, Y., & Wang, X. (2013). Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide. Molecular Medicine Reports, 8, 1411-1415. https://doi.org/10.3892/mmr.2013.1677
MLA
Lv, X., Bi, L., Jiang, Y., Wang, X."Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide". Molecular Medicine Reports 8.5 (2013): 1411-1415.
Chicago
Lv, X., Bi, L., Jiang, Y., Wang, X."Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide". Molecular Medicine Reports 8, no. 5 (2013): 1411-1415. https://doi.org/10.3892/mmr.2013.1677
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