Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide

Lv,Xue-Chao; Bi,Liang-Jia; Jiang,Ying; Wang,Xiang

doi:10.3892/mmr.2013.1677

Effects of icariin on the alkline phosphatase activity of human periodontal ligament cells inhibited by lipopolysaccharide

Authors:
- Xue-Chao Lv
- Liang-Jia Bi
- Ying Jiang
- Xiang Wang
View Affiliations

Affiliations: Department of Pediatric Dentistry, School of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Tooth Planting, School of Stomatology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
Published online on: September 13, 2013 https://doi.org/10.3892/mmr.2013.1677
Pages: 1411-1415

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Icariin (ICA), a flavanoid isolated from herbal Epimedium, has multiple biological activities. The present study investigated the effects of ICA on the proliferation and alkaline phosphatase (ALP) activity (an index for PDLC differentiation) of human periodontal ligament cells (hPDLCs) inhibited by lipopolysaccharide (LPS). hPDLCs were cultured in vitro and stimulated with various concentrations of ICA. The proliferation ability of hPDLCs was detected by an MTT assay. The activity of ALP was determined by the p‑Nitrophenyl phosphate method, and the expression of ALP was analyzed by reverse transcription polymerase chain reaction and western blot analysis. ICA exhibited a dose-dependent effect on the proliferation of hPDLCs in a suitable concentration range, from 10‑6 to 10‑8 mol/l, and with a mediate optimal concentration (10-6 mol/l). The alkaline phosphatase activity was markedly inhibited in 10 µg/ml LPS‑treated PDLCs and this inhibition was suppressed in the presence of icariin at a concentration of 10-6 mol/L following prolonged treatment (96 h). Therefore, this study provided insight into the use of ICA for periapical tissue regeneration.

View Figures

View References

1	Kinane DF and Marshall GJ: Periodontal manifestations of systemic disease. Aust Dent J. 46:2–12. 2001. View Article : Google Scholar
2	Roberts FA, Hockett RD Jr, Bucy RP, et al: Quantitative assessment of inflammatory cytokine gene expression in chronic adult periodontitis. Oral Microbiol Immunol. 12:336–344. 1997. View Article : Google Scholar : PubMed/NCBI
3	Gamonal J, Acevedo A, Bascones A, et al: Levels of interleukin-1 beta, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment. J Periodontol. 71:1535–1545. 2000. View Article : Google Scholar : PubMed/NCBI
4	Thammasitboon K, Goldring SR and Boch JA: Role of macrophages in LPS-induced osteoblast and PDL cell apoptosis. Bone. 38:845–852. 2006. View Article : Google Scholar : PubMed/NCBI
5	Kumada H, Haishima Y, Umemoto T, et al: Structural study on the free lipid A isolated from lipopolysaccharide of Porphyromonas gingivalis. J Bacteriol. 177:2098–2106. 1995.PubMed/NCBI
6	Cho JH, Lee SK, Lee JW and Kim EC: The role of heme oxygenase-1 in mechanical stress-and lipopolysacharide-induced osteogenic differentiation in human periodontal ligament cells. Angle Orthod. 80:552–559. 2010.
7	Murakami Y, Kojima T, Nagasawa T, et al: Novel isolation of alkaline phosphatase-positive subpopulation from periodontal ligament fibroblasts. J Periodontol. 74:780–786. 2003. View Article : Google Scholar : PubMed/NCBI
8	Groeneveld MC, Everts V and Beertsen W: Alkaline phosphatase activity in the periodontal ligament and gingiva of the rat molar: its relation to cementum formation. J Dent Res. 74:1374–1381. 1995. View Article : Google Scholar : PubMed/NCBI
9	Beersten W, vandenBos T and Everts V: Root development in mice lacking functional tissue non-specific alkaline phosphatase gene: inhibition of acellullar cementum formation. J Dent Res. 78:1221–1229. 1999. View Article : Google Scholar : PubMed/NCBI
10	Anan H, Akamine A and Maeda K: An enzyme histochemical study of the behavior of rat bone cells during experimental apical periodontitis. J Endod. 19:83–86. 1993. View Article : Google Scholar : PubMed/NCBI
11	Gibert P, Tramini P, Sieso V and Piva MT: Alkaline phosphatase isoenzyme activity in serum from patients with chronic periodontitis. J Periodontal Res. 38:362–365. 2003. View Article : Google Scholar : PubMed/NCBI
12	Feng-Qiu Zhang, Zhi-Fen Wu, Ling Wan, et al: Effect of lipopolysaccharides on proliferation and alkaline phosphatase activity of periodontal ligament cells. Chinese Journal of Conservative Dentistry. 13:27–19. 2003.(In Chinese).
13	Perinetti G, Paolantonio M, Femminella B, Serra E and Spoto G: Gingival crevicular fluid alkaline phosphatase activity reflects periodontal healing/recurrent inflammation phases in chronic periodontitis patients. J Periodontol. 79:1200–1207. 2008. View Article : Google Scholar
14	Mok SK, Chen WF, Lai WP, et al: Icariin protects against bone loss induced by oestrogen deficiency and activates oestrogen receptor-dependent osteoblastic functions in UMR 106 cells. Br J Pharmacol. 159:939–949. 2010. View Article : Google Scholar : PubMed/NCBI
15	Huang J, Yuan L, Wang X, Zhang TL and Wang K: Icariin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro. Life Sci. 81:832–840. 2007. View Article : Google Scholar : PubMed/NCBI
16	Liu D, Xu J, Liu O, Fan Z, et al: Mesenchymal stem cells derived from inflamed periodontal ligaments exhibit impaired immunomodulation. J Clin Periodontol. 39:1174–1182. 2012. View Article : Google Scholar : PubMed/NCBI
17	Yang SF, Yang ZP and Chang KW: Continuing root formation following apexifiction treatment. Endod Dent Traumatol. 6:232–235. 1990. View Article : Google Scholar : PubMed/NCBI
18	Lekic P and McCulloch CA: Periodontal ligament cell populations: the central role of fibroblasts in creating a unique tissue. Anat Rec. 245:327–341. 1996. View Article : Google Scholar : PubMed/NCBI
19	Chantarawaratit P, Sangvanich P, Banlunara W, Soontornvipart K and Thunyakitpisal P: Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament reneberation in a canine class II furcation defect model. J Periodontal Res. 48:224–232. 2013.PubMed/NCBI
20	Hseih TP, Sheu SY, Sun JS and Chen MH: Icariin inhibits osteoclast differentiation and bone resorption by suppression of MAPKs/NF-κB regulated HIF-1α and PGE(2) synthesis. Phytomedicine. 18:176–185. 2011.PubMed/NCBI
21	Hong CY, Lin SK, Kok SH, et al: The role of lipopolysaccharide in infectious bone resorption of periapical lesion. J Oral Pathol Med. 33:162–169. 2004. View Article : Google Scholar : PubMed/NCBI