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Molecular Medicine Reports
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November 2013 Volume 8 Issue 5

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Article

Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells

  • Authors:
    • Jin Zhang
    • Da-Kang Hu
    • Dong-Guo Wang
    • Yang Liu
    • Chi-Bo Liu
    • Lian-Hua Yu
    • Ying Qu
    • Xin-Hua Luo
    • Jin-Hong Yang
    • Jian Yu
    • Shuang‑Chun Liu
    • Xiang-Yang Li
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, P.R. China, Department of Laboratory Medicine, The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325027, P.R. China
  • Pages: 1570-1574
    |
    Published online on: September 17, 2013
       https://doi.org/10.3892/mmr.2013.1688
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Abstract

Twenty‑three clinical Streptococcus pneumoniae (SP) strains were isolated from blood and sputum specimens from the Second Affiliated Hospital of Wenzhou Medical College in 2009. These strains and the ATCC 49619 standard strain were cultured and suspended in normal saline (at a turbidity of 1.0 McFarland). The production of interleukin (IL)‑8, intracellular adhesion molecule‑1 (ICAM‑1) and IL‑10 in THP‑1 cells following stimulation with the SP suspension was analyzed by an enzyme-linked immunosorbent assay. The concentrations of IL‑8, ICAM‑1 and IL‑10 from the THP‑1 monocytes were greater than those of the blank control following stimulation with the SP suspension. No significant difference was identified in the levels of IL‑8, ICAM‑1 and IL‑10 secretion between THP‑1 monocytes stimulated by blood‑borne SP (bb‑SP) and sputum‑borne SP (sb‑SP).
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Hu D, Wang D, Liu Y, Liu C, Yu L, Qu Y, Luo X, Yang J, Yu J, Yu J, et al: Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Mol Med Rep 8: 1570-1574, 2013.
APA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L. ... Li, X. (2013). Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Molecular Medicine Reports, 8, 1570-1574. https://doi.org/10.3892/mmr.2013.1688
MLA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8.5 (2013): 1570-1574.
Chicago
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8, no. 5 (2013): 1570-1574. https://doi.org/10.3892/mmr.2013.1688
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Hu D, Wang D, Liu Y, Liu C, Yu L, Qu Y, Luo X, Yang J, Yu J, Yu J, et al: Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Mol Med Rep 8: 1570-1574, 2013.
APA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L. ... Li, X. (2013). Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells. Molecular Medicine Reports, 8, 1570-1574. https://doi.org/10.3892/mmr.2013.1688
MLA
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8.5 (2013): 1570-1574.
Chicago
Zhang, J., Hu, D., Wang, D., Liu, Y., Liu, C., Yu, L., Qu, Y., Luo, X., Yang, J., Yu, J., Liu, S., Li, X."Effects of clinical isolates of Streptococcus pneumoniae on THP-1 human monocytic cells". Molecular Medicine Reports 8, no. 5 (2013): 1570-1574. https://doi.org/10.3892/mmr.2013.1688
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