Gankyrin is essential for hypoxia enhanced metastatic potential in breast cancer cells

Gao,Liucun; Xie,Huahong; Dong,Lihou; Zou,Jia; Fu,Jie; Gao,Xin; Ou,Lun; Xiang,Shensi; Song,Haifeng

doi:10.3892/mmr.2013.1860

2014-March Volume 9 Issue 3

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2014-March Volume 9 Issue 3

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Article

Gankyrin is essential for hypoxia enhanced metastatic potential in breast cancer cells

Authors:
- Liucun Gao
- Huahong Xie
- Lihou Dong
- Jia Zou
- Jie Fu
- Xin Gao
- Lun Ou
- Shensi Xiang
- Haifeng Song
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Affiliations: Department of Pharmacology and Toxicology, Beijing Institute of Radiation Medicine, Beijing, P.R. China, State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China
Pages: 1032-1036
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Published online on: December 12, 2013

https://doi.org/10.3892/mmr.2013.1860
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Abstract

Hypoxia, a critical regulator of tumor growth and metastasis, induces the transcriptional activation of several pathways involved in proliferation, migration and invasion. Gankyrin was found to be overexpressed, and also promoted the metastasis in breast cancer cells, which is also involved in the regulation of hypoxia inducible factor‑1 and hypoxia‑inducible factor‑1α. The present study showed that gankyrin mRNA and protein expression were increased under hypoxic conditions in the BT474 breast cancer cell line, accompanied with increased ability of cell migration and invasion. Lentivirus‑mediated siRNA targeting gankyrin was transfected into BT474 cells. Wound‑healing and transwell experiments showed that gankyrin deletion abrogated the increased migration and invasion of BT474 cells due to hypoxia. In addition, E‑cadherin was found to be involved in the gankyrin induced invasion of breast cancer cells due to hypoxia. The present study indicated that gankyrin deletion abrogated the increased metastatic potential of breast cancer cells under hypoxic conditions partly through regulating E‑cadherin, suggesting that an improved understanding of gankyrin may offer a potential therapeutic target for the treatment of human breast cancer metastasis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Gankyrin is essential for hypoxia enhanced metastatic potential in breast cancer cells

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Abstract

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