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Article

Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway

  • Authors:
    • Zhi-Gao Zhang
    • Cheng-Yong Qin
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China
  • Pages: 882-888
    |
    Published online on: December 24, 2013
       https://doi.org/10.3892/mmr.2013.1879
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Abstract

Sirt6, a member of the mammalian sirtuin family, is a protein that is located in the nucleus and is an NAD+‑dependent deacetylase important in the control of metabolic activity and genome stability. Recently, several studies have demonstrated the potential role of Sirt6 in tumor biology; however, the role of Sirt6 in hepatocellular carcinoma (HCC) remains unclear. In the present study, Sirt6 protein expression was found to be downregulated in human HCC tissue compared with adjacent normal tissue. Knockdown of Sirt6 promoted growth of the HepG2 HCC cell line, whereas overexpression of Sirt6 inhibited the growth of HepG2 cells. Overexpression of Sirt6 induced apoptosis in HepG2 cells, which was demonstrated by a terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assay and cleaved caspase-3 immunoblotting. Furthermore, overexpression of Sirt6 decreased intracellular reactive oxygen species and superoxide anion levels. Finally, overexpression of Sirt6 inhibited phosphorylation of extracellular signal-regulated kinases 1/2 (ERK1/2), and blocking the ERK1/2 pathway with chemical-specific inhibitor U0126, attenuated the tumor suppressive effect of overexpression of Sirt6. Collectively, these data suggest that Sirt6 is a tumor suppressor in HCC cells and may be a promising therapeutic target in HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z and Qin C: Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway. Mol Med Rep 9: 882-888, 2014.
APA
Zhang, Z., & Qin, C. (2014). Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway. Molecular Medicine Reports, 9, 882-888. https://doi.org/10.3892/mmr.2013.1879
MLA
Zhang, Z., Qin, C."Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway". Molecular Medicine Reports 9.3 (2014): 882-888.
Chicago
Zhang, Z., Qin, C."Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway". Molecular Medicine Reports 9, no. 3 (2014): 882-888. https://doi.org/10.3892/mmr.2013.1879
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z and Qin C: Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway. Mol Med Rep 9: 882-888, 2014.
APA
Zhang, Z., & Qin, C. (2014). Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway. Molecular Medicine Reports, 9, 882-888. https://doi.org/10.3892/mmr.2013.1879
MLA
Zhang, Z., Qin, C."Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway". Molecular Medicine Reports 9.3 (2014): 882-888.
Chicago
Zhang, Z., Qin, C."Sirt6 suppresses hepatocellular carcinoma cell growth via inhibiting the extracellular signal‑regulated kinase signaling pathway". Molecular Medicine Reports 9, no. 3 (2014): 882-888. https://doi.org/10.3892/mmr.2013.1879
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