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2014-March Volume 9 Issue 3

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Article

Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17

  • Authors:
    • Yuan Xue
    • Yong Yang
    • Zhaoliang Su
    • Prince Amoatt Barnie
    • Dong Zheng
    • Yun Zhang
    • Yan Xu
    • Shengjun Wang
    • Huaxi Xu
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, Institute of Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
  • Pages: 899-903
    |
    Published online on: January 13, 2014
       https://doi.org/10.3892/mmr.2014.1893
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Abstract

Th1 and Th17 cells are involved in the pathogenesis of rheumatoid arthritis (RA). T-bet, a Th1-specific transcription factor, appears to drive the maturation of Th1 and IFN-γ secretion. In the present study, we established the T-bet shRNA recombinant plasmid (p-T-shRNA) and explored its possible anti-inflammatory effect in a collagen-induced arthritis (CIA) model by local injection of plasmid vectors. For the initiation of CIA, DBA/1J mice were immunized with type II collagen (CII) in Freund's adjuvant and the CII-immunized mice were treated with p-T-shRNA. Levels of T-bet, IFN-γ, IL-17 and RORγt mRNA in splenocytes and synovial joints were measured by quantitative real-time PCR and T-bet expression in joint tissue was detected by immunohistochemistry staining. The intracellular IFN-γ and IL-17 were analyzed by flow cytometry (FCM). The results demonstrated that therapeutic administration on the local joints with p-T-shRNA significantly suppressed IFN-γ and IL-17 gene expression and improved the pathogenesis of arthritis in CIA mice, while administration of a plasmid expressing T-bet (pIRES-T-bet) accelerated the disease onset. Our study suggests that T-bet may be developed as a potential target for arthritis therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Xue Y, Yang Y, Su Z, Barnie PA, Zheng D, Zhang Y, Xu Y, Wang S and Xu H: Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17. Mol Med Rep 9: 899-903, 2014.
APA
Xue, Y., Yang, Y., Su, Z., Barnie, P.A., Zheng, D., Zhang, Y. ... Xu, H. (2014). Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17. Molecular Medicine Reports, 9, 899-903. https://doi.org/10.3892/mmr.2014.1893
MLA
Xue, Y., Yang, Y., Su, Z., Barnie, P. A., Zheng, D., Zhang, Y., Xu, Y., Wang, S., Xu, H."Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17". Molecular Medicine Reports 9.3 (2014): 899-903.
Chicago
Xue, Y., Yang, Y., Su, Z., Barnie, P. A., Zheng, D., Zhang, Y., Xu, Y., Wang, S., Xu, H."Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17". Molecular Medicine Reports 9, no. 3 (2014): 899-903. https://doi.org/10.3892/mmr.2014.1893
Copy and paste a formatted citation
x
Spandidos Publications style
Xue Y, Yang Y, Su Z, Barnie PA, Zheng D, Zhang Y, Xu Y, Wang S and Xu H: Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17. Mol Med Rep 9: 899-903, 2014.
APA
Xue, Y., Yang, Y., Su, Z., Barnie, P.A., Zheng, D., Zhang, Y. ... Xu, H. (2014). Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17. Molecular Medicine Reports, 9, 899-903. https://doi.org/10.3892/mmr.2014.1893
MLA
Xue, Y., Yang, Y., Su, Z., Barnie, P. A., Zheng, D., Zhang, Y., Xu, Y., Wang, S., Xu, H."Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17". Molecular Medicine Reports 9.3 (2014): 899-903.
Chicago
Xue, Y., Yang, Y., Su, Z., Barnie, P. A., Zheng, D., Zhang, Y., Xu, Y., Wang, S., Xu, H."Local delivery of T-bet shRNA reduces inflammation in collagen II‑induced arthritis via downregulation of IFN-γ and IL-17". Molecular Medicine Reports 9, no. 3 (2014): 899-903. https://doi.org/10.3892/mmr.2014.1893
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