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Article

Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis

  • Authors:
    • Lei He
    • Bin Wang
    • Yuan Yao
    • Maosheng Su
    • Huanxian Ma
    • Ning Jia
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, PLA General Hospital, Beijing 100853, P.R. China, Department of Critical Care Medicine, PLA General Hospital, Beijing 100853, P.R. China, Department of Medical Quality Control, PLA General Hospital, Beijing 100853, P.R. China, Department of Nosocomial Infection and Disease Control, PLA General Hospital, Beijing 100853, P.R. China
  • Pages: 1869-1876
    |
    Published online on: February 26, 2014
       https://doi.org/10.3892/mmr.2014.1991
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Abstract

Septic shock and sequential multiple organ failure are the main cause of mortality in patients with sepsis. The induction of inflammation during sepsis is a complex biological cascade, which requires successful therapeutic intervention. Selenoprotein S (SEPS1) is a novel endoplasmic reticulum‑resident protein and is important in the production of inflammatory cytokines. The present study attempted to assess the effect of SEPS1 suppression by small interfering RNA (siRNA) on mice with lipopolysaccharide (LPS)‑induced sepsis. In total, 30 mice were randomly assigned to three groups: i) H group (LPS‑induced sepsis group; n=10): Mice with intraperitoneal injection of LPS (10 mg/kg); ii) K group (scrambled siRNA group; n=10): Mice transfected with scrambled control siRNA 12 h prior to injection with LPS and iii) L group (SEPS1 siRNA group; n=10): Mice transfected with SEPS1 siRNA 12 h prior to injection with LPS. The effects of siRNA were evaluated by SEPS1 gene and protein expression, biochemical parameters including serum alanine transaminase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), lactic dehydrogenase (LDH), creatine kinase (CK) and myocardial kinase (CK‑MB), as well as the cytokines interleukin‑6 (IL‑6) and tumor necrosis factor‑α (TNF‑α). The phosphorylation of p38 mitogen‑activated protein kinases (p38 MAPK) was also detected by western blot analysis. In the SEPS1 siRNA group, SEPS1 gene and protein expression decreased significantly, while the levels of TNF‑α and IL‑6 increased compared with the control group. The biochemical parameters of ALT, AST, BUN, LDH, CK and CK‑MB were markedly increased in the SEPS1 siRNA group. The phosphorylation of p38 MAPK was also significantly activated. The decrease in SEPS1 gene and protein expression and the production of TNF‑α and IL‑6 may correlate with the activation of the p38 MAPK pathway. Biochemical factors and pathological results demonstrated that the damage to vital organs was aggravated. In conclusion, these findings suggested that SEPS1 may protect mice against LPS‑induced sepsis and organ damage. Therefore, SEPS1 may be a new target to resolve LPS‑induced sepsis.
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View References

1 

Harrison C: Sepsis: calming the cytokine storm. Nat Rev Drug Discov. 9:360–361. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Delsesto D and Opal SM: Future perspectives on regulating pro-and anti-inflammatory responses in sepsis. Contrib Microbiol. 17:137–156. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Shubin NJ, Monaghan SF and Ayala A: Anti-inflammatory mechanisms of sepsis. Contrib Microbiol. 17:108–124. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Lewis DH, Chan DL, Pinheiro D, Armitage-Chan E and Garden OA: The immunopathology of sepsis: pathogen recognition, systemic inflammation, the compensatory anti-inflammatory response, and regulatory T cells. J Vet Intern Med. 26:457–482. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Lorente JA, Delgado MA and Landin L: Septic shock and nitric oxide. Enferm Infecc Microbiol Clin. 15:14–19. 1997.(In Spanish).

6 

Lvovschi V, Arnaud L, Parizot C, et al: Cytokine profiles in sepsis have limited relevance for stratifying patients in the emergency department: a prospective observational study. PLoS One. 6:e288702011. View Article : Google Scholar : PubMed/NCBI

7 

Ando H, Takamura T, Ota T, Nagai Y and Kobayashi K: Cerivastatin improves survival of mice with lipopolysaccharide-induced sepsis. J Pharmacol Exp Ther. 294:1043–1046. 2000.PubMed/NCBI

8 

Ye Y, Shibata Y, Yun C, Ron D and Rapoport TA: A membrane protein complex mediates retro-translocation from the ER lumen into the cytosol. Nature. 429:841–847. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Ye Y, Shibata Y, Kikkert M, van Voorden S, Wiertz E and Rapoport TA: Recruitment of the p97 ATPase and ubiquitin ligases to the site of retrotranslocation at the endoplasmic reticulum membrane. Proc Natl Acad Sci USA. 102:14132–14138. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Hart K, Landvik NE, Lind H, Skaug V, Haugen A and Zienolddiny S: A combination of functional polymorphisms in the CASP8, MMP1, IL10 and SEPS1 genes affects risk of non-small cell lung cancer. Lung Cancer. 71:123–129. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Curran JE, Jowett JB, Elliott KS, et al: Genetic variation in selenoprotein S influences inflammatory response. Nat Genet. 37:1234–1241. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Rayman MP: Selenoproteins and human health: insights from epidemiological data. Biochim Biophys Acta. 1790:1533–1540. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Kryukov GV, Castellano S, Novoselov SV, et al: Characterization of mammalian selenoproteomes. Science. 300:1439–1443. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Blacker D, Bertram L, Saunders AJ, et al: Results of a high-resolution genome screen of 437 Alzheimer’s disease families. Hum Mol Genet. 12:23–32. 2003.PubMed/NCBI

15 

Field LL, Tobias R and Magnus T: A locus on chromosome 15q26 (IDDM3) produces susceptibility to insulin-dependent diabetes mellitus. Nat Genet. 8:189–194. 1994. View Article : Google Scholar : PubMed/NCBI

16 

Zamani M, Pociot F, Raeymaekers P, Nerup J and Cassiman JJ: Linkage of type I diabetes to 15q26 (IDDM3) in the Danish population. Hum Genet. 98:491–496. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Susi M, Holopainen P, Mustalahti K, Maki M and Partanen J: Candidate gene region 15q26 and genetic susceptibility to coeliac disease in Finnish families. Scand J Gastroenterol. 36:372–374. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Fradejas N, Pastor MD, Mora-Lee S, Tranque P and Calvo S: SEPS1 gene is activated during astrocyte ischemia and shows prominent antiapoptotic effects. J Mol Neurosci. 35:259–265. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Gao Y, Hannan NR, Wanyonyi S, et al: Activation of the selenoprotein SEPS1 gene expression by pro-inflammatory cytokines in HepG2 cells. Cytokine. 33:246–251. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Paddison PJ, Caudy AA and Hannon GJ: Stable suppression of gene expression by RNAi in mammalian cells. Proc Natl Acad Sci USA. 99:1443–1448. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Oberholzer A, Oberholzer C and Moldawer LL: Sepsis syndromes: understanding the role of innate and acquired immunity. Shock. 16:83–96. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Saadeddin SM, Habbab MA and Ferns GA: Markers of inflammation and coronary artery disease. Med Sci Monit. 8:RA5–RA12. 2002.PubMed/NCBI

23 

Bickel C, Rupprecht HJ, Blankenberg S, et al: Relation of markers of inflammation (C-reactive protein, fibrinogen, von Willebrand factor, and leukocyte count) and statin therapy to long-term mortality in patients with angiographically proven coronary artery disease. Am J Cardiol. 89:901–908. 2002. View Article : Google Scholar

24 

de Maat MP and Kluft C: The association between inflammation markers, coronary artery disease and smoking. Vascul Pharmacol. 39:137–139. 2002.PubMed/NCBI

25 

Lin YT, Chen YH, Yang YH, et al: Heme oxygenase-1 suppresses the infiltration of neutrophils in rat liver during sepsis through inactivation of p38 MAPK. Shock. 34:615–621. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Qian F, Deng J, Gantner BN, et al: Map kinase phosphatase 5 protects against sepsis-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 302:L866–L874. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Ruland J and Mak TW: Transducing signals from antigen receptors to nuclear factor kappaB. Immunol Rev. 193:93–100. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
He L, Wang B, Yao Y, Su M, Ma H and Jia N: Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis. Mol Med Rep 9: 1869-1876, 2014.
APA
He, L., Wang, B., Yao, Y., Su, M., Ma, H., & Jia, N. (2014). Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis. Molecular Medicine Reports, 9, 1869-1876. https://doi.org/10.3892/mmr.2014.1991
MLA
He, L., Wang, B., Yao, Y., Su, M., Ma, H., Jia, N."Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis". Molecular Medicine Reports 9.5 (2014): 1869-1876.
Chicago
He, L., Wang, B., Yao, Y., Su, M., Ma, H., Jia, N."Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis". Molecular Medicine Reports 9, no. 5 (2014): 1869-1876. https://doi.org/10.3892/mmr.2014.1991
Copy and paste a formatted citation
x
Spandidos Publications style
He L, Wang B, Yao Y, Su M, Ma H and Jia N: Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis. Mol Med Rep 9: 1869-1876, 2014.
APA
He, L., Wang, B., Yao, Y., Su, M., Ma, H., & Jia, N. (2014). Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis. Molecular Medicine Reports, 9, 1869-1876. https://doi.org/10.3892/mmr.2014.1991
MLA
He, L., Wang, B., Yao, Y., Su, M., Ma, H., Jia, N."Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis". Molecular Medicine Reports 9.5 (2014): 1869-1876.
Chicago
He, L., Wang, B., Yao, Y., Su, M., Ma, H., Jia, N."Protective effects of the SEPS1 gene on lipopolysaccharide-induced sepsis". Molecular Medicine Reports 9, no. 5 (2014): 1869-1876. https://doi.org/10.3892/mmr.2014.1991
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