Component analysis of Pu-erh and its anti-constipation effects

Li,Guijie; Wang,Qiang; Qian,Yu; Zhou,Yalin; Wang,Rui; Zhao,Xin

doi:10.3892/mmr.2014.2009

Component analysis of Pu-erh and its anti-constipation effects

Authors:
- Guijie Li
- Qiang Wang
- Yu Qian
- Yalin Zhou
- Rui Wang
- Xin Zhao
View Affiliations

Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
Published online on: March 5, 2014 https://doi.org/10.3892/mmr.2014.2009
Pages: 2003-2009

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to investigate the effects of Pu-erh tea on activated carbon-induced constipation in ICR mice. The changes in body weight, dietary intake, gastrointestinal transit, first black stool defecation time, number and weight of feces, water content of feces and various levels of substances in serum were used to evaluate the anti-constipation effects of Pu-erh tea. Body weight, dietary intake and the amount of water consumed by mice decreased with activated carbon-induced constipation and those of the sample treated group mice were higher than the control group mice. The first black stool time of normal, control, bisacodyl (100 mg/kg) and 200, 400 and 800 mg/kg Pu-erh tea treated mice were 90, 221, 118, 178, 155 and 139 min, respectively. Following oral administration of 200, 400 and 800 mg/kg of Pu-erh tea and 100 mg/kg of bisacodyl, the gastrointestinal transit rates were shortened by 48.6, 59.6, 78.0 and 91.9%, respectively. Serum levels of motilin (MTL), gastrin (Gas), endothelin (ET), acetylcholine enzyme (AchE), substance P (SP) and vasoactive intestinal peptide (VIP) were significantly increased and somatostatin (SS) was decreased when the mice were treated with different concentrations of Pu-erh tea compared with the untreated control mice. These results demonstrate that Pu-erh tea has a similar preventive effect to bisacodyl and it may be used as a functional food to prevent constipation.

View Figures

View References

1	Weisburger JH: Tea and health: the underlying mechanisms. Proc Soc Exp Biol Med. 220:271–275. 1999. View Article : Google Scholar : PubMed/NCBI
2	Gong ZQ, Watanabe N, Yagi A, Etoh H, Sakata K, Ina K and Liu QJ: Compositional change of Pu-erh tea during processing. Biosci Biotechnol Biochem. 57:1745–1746. 1993. View Article : Google Scholar
3	Duh PD, Yen GC, Yen WJ, Wang BS and Chang LW: Effects of pu-erh tea on oxidative damage and nitric oxide scavenging. J Agric Food Chem. 52:8169–8176. 2004. View Article : Google Scholar : PubMed/NCBI
4	Ueki A and Otsuka M: Life style risks of Parkinson’s disease: Association between decreased water intake and constipation. J Neurol. 251:18–23. 2004.
5	Wexner SD, Beck DE, Baron TH, Fanelli RD, Hyman N, Shen B and Wasco KE: A consensus document on bowel preparation before colonoscopy: prepared by a task force from the American society of colon and rectal surgeons (ASCRS), the American society for gastrointestinal endoscopy (ASGE), and the society of American gastrointestinal and endoscopic surgeons (SAGES). Dis Colon Rectum. 49:792–809. 2006.
6	Farrugia G, Miller SM, Rich A, Liu X, Maines MD, Rae JL and Szurszewski JH: Distribution of heme oxygenase and effects of exogenous carbon monoxide in canine jejunum. Am J Physiol. 274:G350–G358. 1998.PubMed/NCBI
7	Farrugia G, Lei S, Lin X, Miller SM, Nath KA, Ferris CD, Levitt M and Szurszewski JH: A major role for carbon monoxide as an endogenous hyperpolarizing factor in the gastrointestinal tract. Proc Natl Acad Sci USA. 100:8567–8570. 2003. View Article : Google Scholar : PubMed/NCBI
8	Miller SM, Reed D, Sarr MG, Farrugia G and Szurszewski JH: Haem oxygenase in enteric nervous system of human stomach and jejunum and co-localization with nitric oxide synthase. Neurogastroenterol Motil. 13:121–131. 2001. View Article : Google Scholar : PubMed/NCBI
9	Xue L, Farrugia G, Miller SM, Ferris CD, Snyder SH and Szurszewski JH: Carbon monoxide and nitric oxide as coneurotransmitters in the enteric nervous system: evidence from genomic deletion of biosynthetic enzymes. Proc Natl Acad Sci USA. 97:1851–1855. 2000. View Article : Google Scholar : PubMed/NCBI
10	Lee JS, Kim DH, Liu KH, Oh TK and Lee CH: Identification of flavonoids using liquid chromatography with electrospray ionization and ion trap tandem mass spectrometry with an MS/MS library. Rapid Commun Mass Spectrom. 19:3539–3548. 2005. View Article : Google Scholar : PubMed/NCBI
11	Wu SC, Yen GC, Wang BS, Chiu CK, Yen WJ, Chang LW and Du PD: Antimutagenic and antimicrobial activities of pu-erh tea. LWT-Food Sci Technol. 40:506–512. 2007. View Article : Google Scholar
12	Chrubasik C, Maier T, Dawid C, Torda T, Schieber A, Hofmann T and Chrubasik S: An observational study and quantification of the actives in a supplement with Sambucus nigra and Asparagus officinalis used for weight reduction. Phytother Res. 22:913–918. 2008. View Article : Google Scholar : PubMed/NCBI
13	Ghoneim AI and Eldahshan OA: Anti-apoptotic effects of tamarind leaves against ethanol-induced rat liver injury. J Pharm Pharmacol. 64:430–438. 2012. View Article : Google Scholar : PubMed/NCBI
14	Lin MC, Lin JH, Chen SK, Cheng YW and Cheng HW: Simultaneous determination of podophyllotoxin, quercetin and kaempferol in podophyllin by liquid chromatography tandem mass spectrometry. J Food Drug Anal. 16:29–40. 2008.
15	Maki KC, Reeves MS, Farmer M, et al: Green tea catechin consumption enhances exercise-induced abdominal fat loss in overweight and obese adults. J Nutr. 139:264–270. 2009. View Article : Google Scholar : PubMed/NCBI
16	Dolk A, Brodén G, Holmström B, Johansson C and Schultzberg M: Slow transit chronic constipation (Arbuthnot Lane’s disease). An immunohistochemical study of neuropeptide-containing nerves in resected specimens from the large bowel. Int J Colorectal Dis. 5:181–187. 1990.
17	Walia R, Mahajan L and Steffen R: Recent advances in chronic constipation. Curr Opin Pediatr. 21:661–666. 2009. View Article : Google Scholar
18	Emmanuel AV, Tack J, Quigley EM and Talley NJ: Pharmacological management of constipation. Neurogastroenterol Motil. 21:41–54. 2009. View Article : Google Scholar
19	Lubowski DZ, Chen FC, Kennedy ML and King DW: Results of colectomy for severe slow transit constipation. Dis Colon Rectum. 39:23–29. 1996. View Article : Google Scholar
20	Sjölund K, Ekman R, Akre F and Lindner P: Motilin in chronic idiopathic constipation. Scand J Gastroenterol. 21:914–918. 1986.
21	El-Salhy M and Norrgård O: Colonic neuroendocrine peptide levels in patients with chronic idiopathic slow transit constipation. Ups J Med Sci. 103:223–230. 1998. View Article : Google Scholar : PubMed/NCBI
22	Silkoff P, Karmeli F, Goldin E, Ewenson A, Gilon C, Chorev M, Laufer R, Selinger Z and Rachmilewitz D: Effect of substance P on rat gastrointestinal transit. Digest Dis Sci. 33:74–77. 1988. View Article : Google Scholar : PubMed/NCBI
23	Feighner SD, Tan CP, McKee KK, et al: Receptor for motilin identified in the human gastrointestinal system. Science. 284:2184–2188. 1999. View Article : Google Scholar : PubMed/NCBI
24	Preston DM, Adrian TE, Christofides ND, Lennard-Jones JE and Bloom SR: Positive correlation between symptoms and circulating motilin, pancreatic polypeptide and gastrin concentrations in functional bowel disorders. Gut. 26:1059–1064. 1985. View Article : Google Scholar
25	Soudah HC, Hasler WL and Owyang C: Effect of octreotide on intestinal motility and bacterial overgrowth in scleroderma. N Engl J Med. 325:1461–1467. 1991. View Article : Google Scholar : PubMed/NCBI
26	Furchgott RF and Zawadzki JV: The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 288:373–376. 1980. View Article : Google Scholar : PubMed/NCBI
27	Tzavella K, Riepl RL, Klauser AG, Voderholzer WA, Schindlbeck NE and Müller-Lissner SA: Decreased substance P levels in rectal biopsies from patients with slow transit constipation. Eur J Gastroenterol Hepatol. 8:1207–1211. 1996. View Article : Google Scholar : PubMed/NCBI
28	Milner P, Crowe R, Kamm MA, Lennard-Jones JE and Burnstock G: Vasoactive intestinal polypeptide levels in sigmoid colon in idiopathic constipation and diverticular disease. Gastroenterology. 99:666–675. 1990.PubMed/NCBI