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Article

Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2

  • Authors:
    • Ling Liu
    • Jingkai Zhang
    • Meiling Li
    • Xiaohong Zhang
    • Jinlu Zhang
    • Zhenjing Li
    • Likui Wang
    • Jihui Wu
    • Cheng Luo
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Ministry of Education, School of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R. China, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China, School of Life Science, Chinese University of Science and Technology, Hefei, Anhui 230026, P.R. China
  • Pages: 2505-2511
    |
    Published online on: March 17, 2014
       https://doi.org/10.3892/mmr.2014.2059
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Abstract

Cyclooxygenase (COX)-2, a multi-functional molecule, is overexpressed in hepatocellular carcinomas. In order to understand cell proliferation and its association with COX-2 in HepG2 cells in the presence of ursolic acid (UA), viili exopolysaccharides (VEPS) and Astragalus polysaccharides (APS), the cell proliferation, superoxide dismutase (SOD) and metabolic malondialdehyde (MDA) of fatty acids, COX-2, prostaglandin E2 (PGE2), as well as apoptotic morphology and rate were investigated. The results revealed that the activities of SOD, COX-2 and PGE2 were reduced, MDA was markedly decreased, apoptotic blebs were induced, and HepG2 cells were accumulated in the G1 and sub G1/apoptotic phases in test groups. The results indicated that UA, VEPS, APS and any combination of these possess anticancer properties, particularly by downregulating COX-2 expression, which may have increased internal oxidation and triggered apoptosis together with a change in internal antioxidant response elements, leading to a reduction in cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Zhang J, Li M, Zhang X, Zhang J, Li Z, Wang L, Wu J and Luo C: Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2. Mol Med Rep 9: 2505-2511, 2014.
APA
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z. ... Luo, C. (2014). Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2. Molecular Medicine Reports, 9, 2505-2511. https://doi.org/10.3892/mmr.2014.2059
MLA
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z., Wang, L., Wu, J., Luo, C."Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2". Molecular Medicine Reports 9.6 (2014): 2505-2511.
Chicago
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z., Wang, L., Wu, J., Luo, C."Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2". Molecular Medicine Reports 9, no. 6 (2014): 2505-2511. https://doi.org/10.3892/mmr.2014.2059
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Zhang J, Li M, Zhang X, Zhang J, Li Z, Wang L, Wu J and Luo C: Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2. Mol Med Rep 9: 2505-2511, 2014.
APA
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z. ... Luo, C. (2014). Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2. Molecular Medicine Reports, 9, 2505-2511. https://doi.org/10.3892/mmr.2014.2059
MLA
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z., Wang, L., Wu, J., Luo, C."Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2". Molecular Medicine Reports 9.6 (2014): 2505-2511.
Chicago
Liu, L., Zhang, J., Li, M., Zhang, X., Zhang, J., Li, Z., Wang, L., Wu, J., Luo, C."Inhibition of HepG2 cell proliferation by ursolic acid and polysaccharides via the downregulation of cyclooxygenase-2". Molecular Medicine Reports 9, no. 6 (2014): 2505-2511. https://doi.org/10.3892/mmr.2014.2059
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