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Article

Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells

  • Authors:
    • Ok-Yeon Kim
    • Hwa Jun Cha
    • Kyu Joong Ahn
    • In-Sook An
    • Sungkwan An
    • Seunghee Bae
  • View Affiliations / Copyright

    Affiliations: Molecular-Targeted Drug Research Center, Konkuk University, Seoul 143-701, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea, Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea
  • Pages: 145-154
    |
    Published online on: April 16, 2014
       https://doi.org/10.3892/mmr.2014.2158
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Abstract

microRNAs (miRNAs) are small non‑coding RNAs that regulate various biological processes by interfering with the translation of target genes. Several studies have suggested that miRNAs are involved in cellular responses to hydrogen peroxide (H2O2). Reactive oxygen species (ROS) are involved in hair malignancies, however, the H2O2‑induced, miRNA‑dependent regulatory mechanisms of human dermal papilla (HDP) cells are not fully understood. Our previous study demonstrated that changes in miRNA expression function to regulate growth arrest and apoptosis in UVB‑irradiated HDPs. In the present study, miRNA expression was profiled in HDPs treated with H2O2. The transcriptome analysis of H2O2‑treated HDPs enabled the identification of 68 differentially expressed miRNAs (62 were upregulated and 6 were downregulated) and 14,316 putative target genes of the miRNAs. Gene ontology (GO) analysis was utilized to verify that the putative target genes of the altered miRNAs were associated with H2O2‑induced cell growth arrest and apoptosis. This bioinformatics analysis indicated that H2O2‑response pathways involved in growth arrest and apoptosis were significantly affected. The identification of miRNAs and their putative targets may offer new therapeutic strategies for H2O2‑induced hair follicle disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Kim O, Cha HJ, Ahn KJ, An I, An S and Bae S: Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells. Mol Med Rep 10: 145-154, 2014.
APA
Kim, O., Cha, H.J., Ahn, K.J., An, I., An, S., & Bae, S. (2014). Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells. Molecular Medicine Reports, 10, 145-154. https://doi.org/10.3892/mmr.2014.2158
MLA
Kim, O., Cha, H. J., Ahn, K. J., An, I., An, S., Bae, S."Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells". Molecular Medicine Reports 10.1 (2014): 145-154.
Chicago
Kim, O., Cha, H. J., Ahn, K. J., An, I., An, S., Bae, S."Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells". Molecular Medicine Reports 10, no. 1 (2014): 145-154. https://doi.org/10.3892/mmr.2014.2158
Copy and paste a formatted citation
x
Spandidos Publications style
Kim O, Cha HJ, Ahn KJ, An I, An S and Bae S: Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells. Mol Med Rep 10: 145-154, 2014.
APA
Kim, O., Cha, H.J., Ahn, K.J., An, I., An, S., & Bae, S. (2014). Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells. Molecular Medicine Reports, 10, 145-154. https://doi.org/10.3892/mmr.2014.2158
MLA
Kim, O., Cha, H. J., Ahn, K. J., An, I., An, S., Bae, S."Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells". Molecular Medicine Reports 10.1 (2014): 145-154.
Chicago
Kim, O., Cha, H. J., Ahn, K. J., An, I., An, S., Bae, S."Identification of microRNAs involved in growth arrest and cell death in hydrogen peroxide-treated human dermal papilla cells". Molecular Medicine Reports 10, no. 1 (2014): 145-154. https://doi.org/10.3892/mmr.2014.2158
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