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Article

Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes

  • Authors:
    • Hao Chen
    • Xiaomei Zhang
    • Yifan Feng
    • Wen Rui
    • Zhongfeng Shi
    • Lirong Wu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Center Laboratory, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China
  • Pages: 1355-1362
    |
    Published online on: June 16, 2014
       https://doi.org/10.3892/mmr.2014.2331
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Abstract

Glycyrrhiza uralensis (G. uralensis) is a common medicinal plant that has mainly been used to modulate the pharmaceutical activity of herbal medicines. Although G. uralensis has been shown to affect the expression and activity of the key metabolic enzyme cytochrome P450 (CYP450), the detailed mechanism of this process has yet to be elucidated. The present study aimed to elucidate the effects of bioactive components of G. uralensis on different isoforms of CYP450 and determine the ability of these components to modulate drug properties. In the present study, mRNA levels of CYP1A2, CYP2D6, CYP2E1, and CYP3A4 were investigated by quantitative polymerase chain reaction (qPCR) in HepG2 cells following treatment with the major bioactive compounds of G. uralensis. The activity of CYP450 enzymes was investigated in human liver microsomes using the cocktail probe drug method, and the metabolites of specific probes were detected by UPLC‑MS/MS. The effects of G. uralensis on CYP450 were assessed using bioinformatics network analysis. Several compounds from G. uralensis had various effects on the expression and activity of multiple CYP450 isoforms. The majority of the compounds analysed the inhibited expression of CYP2D6 and CYP3A4. Several CYP isoforms were differentially modulated depending on the specific compound and dose tested. In conclusion, the present study suggested that G. uralensis influenced the expression and activity of CYP450 enzymes. Therefore, caution should be taken when G. uralensis is co‑administered with drugs that are known to be metabolized by CYP450. This study contributed to the knowledge of the mechanisms by which this medicinal plant, commonly known as licorice, modulates drug efficacy.
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Copy and paste a formatted citation
Spandidos Publications style
Chen H, Zhang X, Feng Y, Rui W, Shi Z and Wu L: Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes. Mol Med Rep 10: 1355-1362, 2014.
APA
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., & Wu, L. (2014). Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes. Molecular Medicine Reports, 10, 1355-1362. https://doi.org/10.3892/mmr.2014.2331
MLA
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., Wu, L."Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes". Molecular Medicine Reports 10.3 (2014): 1355-1362.
Chicago
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., Wu, L."Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes". Molecular Medicine Reports 10, no. 3 (2014): 1355-1362. https://doi.org/10.3892/mmr.2014.2331
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Zhang X, Feng Y, Rui W, Shi Z and Wu L: Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes. Mol Med Rep 10: 1355-1362, 2014.
APA
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., & Wu, L. (2014). Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes. Molecular Medicine Reports, 10, 1355-1362. https://doi.org/10.3892/mmr.2014.2331
MLA
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., Wu, L."Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes". Molecular Medicine Reports 10.3 (2014): 1355-1362.
Chicago
Chen, H., Zhang, X., Feng, Y., Rui, W., Shi, Z., Wu, L."Bioactive components of Glycyrrhiza uralensis mediate drug functions and properties through regulation of CYP450 enzymes". Molecular Medicine Reports 10, no. 3 (2014): 1355-1362. https://doi.org/10.3892/mmr.2014.2331
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