CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia

Xing,Limin; Liu,Chunyan; Fu,Rong; Wang,Huaquan; Wang,Jun; Liu,Xiao; Feng,Le; Li,Lijuan; Liu,Hui; Wang,Honglei; Zhang,Tian; Shao,Zonghong

doi:10.3892/mmr.2014.2344

September-2014 Volume 10 Issue 3

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September-2014 Volume 10 Issue 3

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Article

CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia

Authors:
- Limin Xing
- Chunyan Liu
- Rong Fu
- Huaquan Wang
- Jun Wang
- Xiao Liu
- Le Feng
- Lijuan Li
- Hui Liu
- Honglei Wang
- Tian Zhang
- Zonghong Shao
View Affiliations / Copyright

Affiliations: Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, P.R. China
Pages: 1252-1258
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Published online on: June 18, 2014

https://doi.org/10.3892/mmr.2014.2344
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Abstract

The aim of the present study was to investigate the number and function of CD8+HLA-DR+ cells, which are considered to be activated cytotoxic T lymphocytes (CTLs), in peripheral blood to further examine the pathogenesis of severe aplastic anemia (SAA). Thirty-eight patients with SAA were included in the present study. Patients were screened for paroxysmal nocturnal hemoglobinuria by flow cytometry using anti-CD55 and anti-CD59 antibodies. The number of CD8+HLA-DR+ T cells was measured by three-color flow cytometry using anti-CD8-peridinin chlorophyll, anti-CD3-fluorescein isothiocyanate (FITC) and anti-HLA-DR-FITC antibodies. The expression of perforin, granzyme B, tumor necrosis factor-β (TNF-β) and FasL in CD8+HLA-DR+ T cells was detected by flow cytometry with the appropriate monoclonal antibodies. Total RNA was prepared from purified CD8+HLA-DR+ cells of healthy controls and SAA patients, and then polymerase chain reaction (PCR) was performed. Apoptosis of CD8+HLA-DR+ cells was detected by flow cytometry following staining with Annexin V. The proportion of CD8+HLA-DR+ T cells was analyzed by flow cytometry in peripheral blood and was identified to be significantly higher in untreated SAA than in remission patients and in the controls. The expression of perforin, granzyme B, TNF-β and FasL in CD8+HLA-DR+ T cells was analyzed by flow cytometry and PCR, which revealed increased expression in the untreated SAA group compared with that in the control group. Furthermore, the apoptosis of CD3- bone marrow cells from normal individuals was enhanced following co-culture with CD8+HLA-DR+ T cells from untreated SAA patients. In conclusion, the present study demonstrated that CD8+HLA-DR+ T cells may contribute to bone marrow failure in SAA.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia

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