CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia

  • Authors:
    • Limin Xing
    • Chunyan Liu
    • Rong Fu
    • Huaquan Wang
    • Jun Wang
    • Xiao Liu
    • Le Feng
    • Lijuan Li
    • Hui Liu
    • Honglei Wang
    • Tian Zhang
    • Zonghong Shao
  • View Affiliations

  • Published online on: June 18, 2014     https://doi.org/10.3892/mmr.2014.2344
  • Pages: 1252-1258
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Abstract

The aim of the present study was to investigate the number and function of CD8+HLA-DR+ cells, which are considered to be activated cytotoxic T lymphocytes (CTLs), in peripheral blood to further examine the pathogenesis of severe aplastic anemia (SAA). Thirty-eight patients with SAA were included in the present study. Patients were screened for paroxysmal nocturnal hemoglobinuria by flow cytometry using anti-CD55 and anti-CD59 antibodies. The number of CD8+HLA-DR+ T cells was measured by three-color flow cytometry using anti-CD8-peridinin chlorophyll, anti-CD3-fluorescein isothiocyanate (FITC) and anti-HLA-DR-FITC antibodies. The expression of perforin, granzyme B, tumor necrosis factor-β (TNF-β) and FasL in CD8+HLA-DR+ T cells was detected by flow cytometry with the appropriate monoclonal antibodies. Total RNA was prepared from purified CD8+HLA-DR+ cells of healthy controls and SAA patients, and then polymerase chain reaction (PCR) was performed. Apoptosis of CD8+HLA-DR+ cells was detected by flow cytometry following staining with Annexin V. The proportion of CD8+HLA-DR+ T cells was analyzed by flow cytometry in peripheral blood and was identified to be significantly higher in untreated SAA than in remission patients and in the controls. The expression of perforin, granzyme B, TNF-β and FasL in CD8+HLA-DR+ T cells was analyzed by flow cytometry and PCR, which revealed increased expression in the untreated SAA group compared with that in the control group. Furthermore, the apoptosis of CD3- bone marrow cells from normal individuals was enhanced following co-culture with CD8+HLA-DR+ T cells from untreated SAA patients. In conclusion, the present study demonstrated that CD8+HLA-DR+ T cells may contribute to bone marrow failure in SAA.
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September-2014
Volume 10 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Xing L, Liu C, Fu R, Wang H, Wang J, Liu X, Feng L, Li L, Liu H, Wang H, Wang H, et al: CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia. Mol Med Rep 10: 1252-1258, 2014
APA
Xing, L., Liu, C., Fu, R., Wang, H., Wang, J., Liu, X. ... Shao, Z. (2014). CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia. Molecular Medicine Reports, 10, 1252-1258. https://doi.org/10.3892/mmr.2014.2344
MLA
Xing, L., Liu, C., Fu, R., Wang, H., Wang, J., Liu, X., Feng, L., Li, L., Liu, H., Wang, H., Zhang, T., Shao, Z."CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia". Molecular Medicine Reports 10.3 (2014): 1252-1258.
Chicago
Xing, L., Liu, C., Fu, R., Wang, H., Wang, J., Liu, X., Feng, L., Li, L., Liu, H., Wang, H., Zhang, T., Shao, Z."CD8+HLA-DR+ T cells are increased in patients with severe aplastic anemia". Molecular Medicine Reports 10, no. 3 (2014): 1252-1258. https://doi.org/10.3892/mmr.2014.2344