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Article

Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis

  • Authors:
    • Chen Liu
    • Tao Hong
    • Mengping Shao
    • Zhenghang Chen
    • Chunsheng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P.R. China
  • Pages: 1323-1328
    |
    Published online on: July 15, 2014
       https://doi.org/10.3892/mmr.2014.2382
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Abstract

Melatonin, a widespread physiological mediator, has been demonstrated to exhibit a dose‑dependent immunoregulatory effect in vitro and in vivo, including mediating physiological circadian rhythms, neutralizing free radicals and exerting antisenescence actions. In the present study, the efficacy and mechanism of melatonin alone or in combination with cyclosporine (CsA) in prolonging heart transplantation survival was examined. Daily treatment with melatonin (200 mg/kg/day) through a gavage, significantly prolonged the survival of cardiac grafts (mean survival time, 13.4±2.4 days; n=7; P<0.0001) compared with the untreated controls (5.8±1.2 days; n=7). When CsA (5 mg/kg/day) was co‑administered with melatonin (50 mg/kg/day), the survival rate improved (31.6±2.4 days; n=7; P<0.001), compared with that achieved by only 20 mg/kg/day CsA (22±2.8 days; n=7). As expected, melatonin significantly alleviated the inflammatory response and apoptosis as determined by TdT‑mediated dUTP‑biotin nick end labeling assay (P<0.05). Further analysis demonstrated that melatonin significantly reduced p65 activation and the release of inflammatory factors. Therefore, these findings indicate that melatonin in combination with CsA protected the cardiac allograft by inhibiting inflammation‑induced apoptosis. These results provide evidence for a novel therapeutic approach for future immunosuppressive agents in organ transplantation.
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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Hong T, Shao M, Chen Z and Wang C: Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Mol Med Rep 10: 1323-1328, 2014.
APA
Liu, C., Hong, T., Shao, M., Chen, Z., & Wang, C. (2014). Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Molecular Medicine Reports, 10, 1323-1328. https://doi.org/10.3892/mmr.2014.2382
MLA
Liu, C., Hong, T., Shao, M., Chen, Z., Wang, C."Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis". Molecular Medicine Reports 10.3 (2014): 1323-1328.
Chicago
Liu, C., Hong, T., Shao, M., Chen, Z., Wang, C."Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis". Molecular Medicine Reports 10, no. 3 (2014): 1323-1328. https://doi.org/10.3892/mmr.2014.2382
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Hong T, Shao M, Chen Z and Wang C: Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Mol Med Rep 10: 1323-1328, 2014.
APA
Liu, C., Hong, T., Shao, M., Chen, Z., & Wang, C. (2014). Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis. Molecular Medicine Reports, 10, 1323-1328. https://doi.org/10.3892/mmr.2014.2382
MLA
Liu, C., Hong, T., Shao, M., Chen, Z., Wang, C."Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis". Molecular Medicine Reports 10.3 (2014): 1323-1328.
Chicago
Liu, C., Hong, T., Shao, M., Chen, Z., Wang, C."Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis". Molecular Medicine Reports 10, no. 3 (2014): 1323-1328. https://doi.org/10.3892/mmr.2014.2382
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