Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
September-2014 Volume 10 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2014 Volume 10 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

(-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase

  • Authors:
    • Yuko Iida
    • Tomoaki Doi
    • Rie Matsushima-Nishiwaki
    • Haruhiko Tokuda
    • Shinji Ogura
    • Osamu Kozawa
    • Hiroki Iida
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Gifu University Graduate School of Medicine, Gifu, Gifu 501-1194, Japan, Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu 501-1194, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Gifu 501-1194, Japan
  • Pages: 1383-1388
    |
    Published online on: July 16, 2014
       https://doi.org/10.3892/mmr.2014.2389
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

(‑)‑Epigallocatechin gallate (EGCG) is a major component of green tea. It has been demonstrated that EGCG has an antithrombotic effect by inhibiting platelet aggregation. However, the detailed mechanisms underlying the effects of EGCG remain to be elucidated. The present study examined the effects of EGCG on human platelet activation by various stimulators and the exact underlying mechanisms. EGCG suppressed adenosine diphosphate (ADP)‑stimulated platelet aggregation dose dependently between 30 and 70 µM. By contrast, EGCG failed to affect platelet aggregation stimulated by collagen, U46619 (a TP agonist) or ristocetin (an activator of GPIb/IX/V). EGCG attenuated the ADP‑induced phosphorylation of p38 mitogen‑activated protein (MAP) kinase and heat shock protein 27 (HSP27). The ADP‑stimulated release of platelet‑derived growth factor (PDGF)‑AB and the soluble CD40 (sCD40) ligand was inhibited by EGCG. These findings suggest that EGCG selectively inhibits ADP‑stimulated human platelet activation and that EGCG reduces the release of PDGF‑AB and the sCD40 ligand due to suppressing HSP27 phosphorylation via p38 MAP kinase.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Jankun J, Selman SH, Swiercz R and Skrzypczak-Jankun E: Why drinking green tea could prevent cancer. Nature. 387:5611997. View Article : Google Scholar : PubMed/NCBI

2 

Kang WS, Lim IH, Yuk DY, et al: Antithrombotic activities of green tea catechins and (−)-epigallocatechin gallate. Thromb Res. 96:229–237. 1999.

3 

Oyama J, Maeda T, Kouzuma K, et al: Green tea catechins improve human forearm endothelial dysfunction and have antiatherosclerotic effects in smokers. Circ J. 74:578–588. 2010. View Article : Google Scholar

4 

Harborne JB and Williams CA: Advances in flavonoid research since 1992. Phytochemistry. 55:418–504. 2000. View Article : Google Scholar

5 

Ruggeri ZM: The role of von Willebrand factor in thrombus formation. Thromb Res. 120:S5–S9. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Berndt MC, Shen Y, Dopheide SM, Gardiner EE and Andrews RK: The vascular biology of the glycoprotein Ib-IX-V complex. Thromb Haemost. 86:178–188. 2001.PubMed/NCBI

7 

Garcia A, Quinton TM, Dorsam RT and Kunapuli SP: Src family kinase-mediated and Erk-mediated thromboxane A2 generation are essential for VWF/GPIb-induced fibrinogen receptor activation in human platelets. Blood. 106:3410–3414. 2005. View Article : Google Scholar

8 

Jennings LK: Mechanisms of platelet activation: need for new strategies to protect against platelet-mediated atherothrombosis. Thromb Haemost. 102:248–257. 2009.

9 

Dong JF, Berndt MC, Schade A, McIntire LV, Andrews RK and López JA: Ristocetin-dependent, but not botrocetin-dependent, binding of von Willebrand factor to the platelet glycoprotein Ib-IX-V complex correlates with shear-dependent interactions. Blood. 97:162–168. 2001. View Article : Google Scholar

10 

Hechler B, Léon C, Vial C, Vigne P, Frelin C, Cazenave JP and Gachet C: The P2Y1 receptor is necessary for adenosine 5′-diphosphate-induced platelet aggregation. Blood. 92:152–159. 1998.

11 

Daniel JL, Dangelmaier C, et al: Role of intracellular signaling events in ADP-induced platelet aggregation. Thromb Haemost. 82:1322–1326. 1999.PubMed/NCBI

12 

Kato H, Takai S, Matsushima-Nishiwaki R, Adachi S, Minamitani C, Otsuka T, Tokuda H, Akamatsu S, Doi T, Ogura S and Kozawa O: HSP27 phosphorylation is correlated with ADP-induced platelet granule secretion. Arch Biochem Biophys. 475:80–86. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Doi T, Adachi S, Matsushima-Nishiwaki R, Kato H, Enomoto Y, Minamitani C, Otsuka T, Tokuda H, Akamatsu S, Iwama T, Kozawa O and Ogura S: Antithrombin III suppresses ADP-induced platelet granule secretion: inhibition of HSP27 phosphorylation. Arch Biochem Biophys. 489:62–67. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Enomoto Y, Adachi S, Matsushima-Nishiwaki R, Doi T, Niwa M, Akamastu S, Tokuda H, Ogura S, Yoshimura S, Iwama T and Kozawa O: Thromboxane A(2) promotes soluble CD40 ligand release from human platelets. Atherosclerosis. 209:415–421. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Jin YR, Im JH, Park ES, Cho MR, Han XH, Lee JJ, Lim Y, Kim TJ and Yun YP: Antiplatelet activity of epigallocatechin gallate is mediated by the inhibition of PLCgamma2 phosphorylation, elevation of PGD2 production, and maintaining calcium-ATPase activity. J Cardiovasc Pharmacol. 51:45–54. 2008. View Article : Google Scholar

16 

Fabre JE, Nguyen M, Latour A, Keifer JA, Audoly LP, Coffman TM and Koller BH: Decreased platelet aggregation, increased bleeding time and resistance to thromboembolism in P2Y1-deficient mice. Nat Med. 5:1199–1202. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Kato K, Ito H, Hasegawa K, Inaguma Y, Kozawa O and Asano T: Modulation of the stress-induced synthesis of hsp27 and αB-crystallin by cyclic AMP in C6 rat glioma cells. J Neurochem. 66:946–950. 1996.

18 

Laemmli UK: Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature. 227:680–685. 1970. View Article : Google Scholar : PubMed/NCBI

19 

Liu J, Pestina TI, Berndt MC, Steward SA, Jackson CW and Gartner TK: The roles of ADP and TXA2 in botrocetin/VWF-induced aggregation of washed platelets. J Thromb Haemost. 2:2213–2222. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Nakahata N: Thromboxane A2: physiology/pathophysiology, cellular signal transduction and pharmacology. Pharmacol Ther. 118:18–35. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Rendu F and Brohard-Bohn B: The platelet release reaction: granules’ constituents, secretion and functions. Platelets. 12:261–273. 2001.

22 

Heldin CH and Westermark B: Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev. 79:1283–1316. 1999.PubMed/NCBI

23 

Hermann A, Rauch BH, Braun M, Schrör K and Weber AA: Platelet CD40 ligand (CD40L) - subcellular localization, regulation of expression, and inhibition by clopidogrel. Platelets. 12:74–82. 2001. View Article : Google Scholar : PubMed/NCBI

24 

André P, Nannizzi-Alaimo L, Prasad SK and Phillips DR: Platelet-derived CD40L: the switch-hitting player of cardiovascular disease. Circulation. 106:896–899. 2002.PubMed/NCBI

25 

Henn V, Slupsky JR, Gräfe M, Anagnostopoulos I, Förster R, Müller-Berghaus G and Kroczek RA: CD40 ligand on activated platelets triggers an inflammatory reaction of endothelial cells. Nature. 391:591–594. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Heeschen C, Dimmeler S, Hamm CW, van den Brand MJ, Boersma E, Zeiher AM and Simoons ML; CAPTURE Study Investigators. Soluble CD40 ligand in acute coronary syndromes. N Engl J Med. 348:1104–1111. 2003. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Iida Y, Doi T, Matsushima-Nishiwaki R, Tokuda H, Ogura S, Kozawa O and Iida H: (-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase. Mol Med Rep 10: 1383-1388, 2014.
APA
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., & Iida, H. (2014). (-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase. Molecular Medicine Reports, 10, 1383-1388. https://doi.org/10.3892/mmr.2014.2389
MLA
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., Iida, H."(-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase". Molecular Medicine Reports 10.3 (2014): 1383-1388.
Chicago
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., Iida, H."(-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase". Molecular Medicine Reports 10, no. 3 (2014): 1383-1388. https://doi.org/10.3892/mmr.2014.2389
Copy and paste a formatted citation
x
Spandidos Publications style
Iida Y, Doi T, Matsushima-Nishiwaki R, Tokuda H, Ogura S, Kozawa O and Iida H: (-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase. Mol Med Rep 10: 1383-1388, 2014.
APA
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., & Iida, H. (2014). (-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase. Molecular Medicine Reports, 10, 1383-1388. https://doi.org/10.3892/mmr.2014.2389
MLA
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., Iida, H."(-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase". Molecular Medicine Reports 10.3 (2014): 1383-1388.
Chicago
Iida, Y., Doi, T., Matsushima-Nishiwaki, R., Tokuda, H., Ogura, S., Kozawa, O., Iida, H."(-)-Epigallocatechin gallate selectively inhibits adenosine diphosphate‑stimulated human platelet activation: Suppression of heat shock protein 27 phosphorylation via p38 mitogen‑activated protein kinase". Molecular Medicine Reports 10, no. 3 (2014): 1383-1388. https://doi.org/10.3892/mmr.2014.2389
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team