Histone deacetylase 5 promotes the proliferation of glioma cells by upregulation of Notch 1

Liu,Quan; Zheng,Jie‑Min; Chen,Jia‑Kang; Yan,Xian‑Lei; Chen,Hong‑Mou; Nong,Wei‑Xia; Huang,He‑Qing

doi:10.3892/mmr.2014.2395

October 2014 Volume 10 Issue 4

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October 2014 Volume 10 Issue 4

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Article

Histone deacetylase 5 promotes the proliferation of glioma cells by upregulation of Notch 1

Authors:
- Quan Liu
- Jie‑Min Zheng
- Jia‑Kang Chen
- Xian‑Lei Yan
- Hong‑Mou Chen
- Wei‑Xia Nong
- He‑Qing Huang
View Affiliations / Copyright

Affiliations: Department of Neurosurgery, The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, P.R. China, Department of Histology and Embryology, School of Basic Medicine, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Pages: 2045-2050
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Published online on: July 18, 2014

https://doi.org/10.3892/mmr.2014.2395
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Abstract

Histone deacetylases (HDACs) constitute a family of enzymes that play important roles in the epigenetic regulation of gene expression and contribute to the growth, differentiation and apoptosis of cancer cells. However, the biological function of HDAC5 in glioma cells has not been fully understood. In the present study, we found that the mRNA and protein levels of HDAC5 are increased in human glioma tissues and cell lines. In addition, overexpression of HDAC5 promoted proliferation of glioma cells, as measured by the MTT assay. By contrast, HDAC5 gene silencing using small interfering RNA (siRNA) inhibited cell proliferation. Furthermore, we demonstrated that HDAC5 enhances Notch 1 expression at both the mRNA and the protein level in glioma cell lines. Taken together, these results demonstrated, for the first time to the best of our knowledge, that HDAC5 promotes glioma cell proliferation, and suggest that this effect involves the upregulation of Notch 1. Therefore, our study may provide a novel therapeutic target for treatment of gliomas.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Histone deacetylase 5 promotes the proliferation of glioma cells by upregulation of Notch 1

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