Anthocyanins potentiate the activity of trastuzumab in human epidermal growth factor receptor 2‑positive breast cancer cells in vitro and in vivo

Liu,Weihua; Xu,Jinmei; Liu,Yilun; Yu,Xiaoping; Tang,Xi; Wang,Zhi; Li,Xin

doi:10.3892/mmr.2014.2414

October 2014 Volume 10 Issue 4

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

October 2014 Volume 10 Issue 4

Full Size Image

Article

Anthocyanins potentiate the activity of trastuzumab in human epidermal growth factor receptor 2‑positive breast cancer cells in vitro and in vivo

Authors:
- Weihua Liu
- Jinmei Xu
- Yilun Liu
- Xiaoping Yu
- Xi Tang
- Zhi Wang
- Xin Li
View Affiliations / Copyright

Affiliations: Department of Scientific Research, First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Department of Public Health, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Administrative Office, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China
Pages: 1921-1926
|
Published online on: July 22, 2014

https://doi.org/10.3892/mmr.2014.2414
Expand metrics +

Abstract

Human epidermal growth factor receptor 2 (HER2) has been found to be overexpressed in ~25% of invasive breast cancer and is significantly associated with a poor prognosis in breast cancer patients. The anthocyanins cyanidin‑3‑glucoside (C3G) and peonidin‑3‑glucoside have been identified as potential drugs for the therapy of HER2‑positive breast cancer. They have been used as supplements in targeted therapeutics and chemotherapeutics in Asia, however, the underlying mechanism remains to be elucidated. The aim of the present study was to investigate the synergism between C3G and trastuzumab (Trast). To address this question, the response to C3G, Trast and a combination of the two drugs, in three representative HER2‑positive cell lines was evaluated. The combination treatments induced apoptosis, inhibited cell growth and affected HER2 and its downstream signaling pathway in MDA‑MB‑453, BT474 and HCC1569 cells, and the effects were synergistic. The combination of 3CG and Trast inhibited tumor growth in an in vivo xenograft model. The data from the present study suggested that C3G exhibits potent antitumor activity when combined with Trast under the investigated conditions.

View Figures

View References

1	American Cancer Society. Cancer Facts & Figures 2013. American Cancer Society; Atlanta: 2013
2	Lv F, Yu Y, Zhang B, Liang D, Li ZM and You W: Inhibitory effects of mild hyperthermia plus docetaxel therapy on ER(+/-) breast cancer cells and action mechanisms. J Huazhong Univ Sci Technolog Med Sci. 33:870–876. 2013. View Article : Google Scholar : PubMed/NCBI
3	Vrbic S, Pejcic I, Filipovic S, Kocic B and Vrbic M: Current and future anti-HER2 therapy in breast cancer. J BUON. 18:4–16. 2013.PubMed/NCBI
4	Marty M, Cognetti F, Maraninchi D, et al: Randomized phase II trial of the efficacy and safety of trastuzumab combined with docetaxel in patients with human epidermal growth factor receptor 2-positive metastatic breast cancer administered as first-line treatment: the M77001 study group. J Clin Oncol. 23:4265–4274. 2005. View Article : Google Scholar
5	Neve RM, Chin K, Fridlyand J, et al: A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. Cancer Cell. 10:515–527. 2006. View Article : Google Scholar : PubMed/NCBI
6	Slamon DJ, Leyland-Jones B, Shak S, et al: Use of chemotherapy plus a monoclonal antibody against HER2 for metastatic breast cancer that overexpresses HER2. N Eng J Med. 344:783–792. 2001. View Article : Google Scholar : PubMed/NCBI
7	Huang C, Lee SY, Lin CL, et al: Co-treatment with quercetin and 1,2,3,4,6-penta-O-galloyl-β-d-glucose causes cell cycle arrest and apoptosis in human breast cancer MDA-MB-231 and AU565 cells. J Agric Food Chem. 61:6430–6445. 2013.PubMed/NCBI
8	Wu XQ, Shao SJ and Qu WC: Effects of ru’ai shuhou recipe on the matrix metalloproteinases and the inhibitive factors in the recurrence and metastasis of HER2 positive breast cancer. Zhongguo Zhong Xi Yi Jie He Za Zhi Zhongguo Zhongxiyi Jie He Za Zhi. 32:1526–1530. 2012.(In Chinese).
9	Liu W, Xu J, Wu S, et al: Selective anti-proliferation of HER2-positive breast cancer cells by anthocyanins identified by high-throughput screening. PloS One. 8:e815862013. View Article : Google Scholar : PubMed/NCBI
10	Chen XQ, Nagao N, Itani T and Irifune K: Anti-oxidative analysis and identification and quantification of anthocyanin pigments in different coloured rice. Food chem. 135:2783–2788. 2012. View Article : Google Scholar : PubMed/NCBI
11	Fernandes I, Faria A, Azevedo J, et al: Influence of anthocyanins, derivative pigments and other catechol and pyrogallol-type phenolics on breast cancer cell proliferation. J Agric Food Chem. 58:3785–3792. 2010. View Article : Google Scholar : PubMed/NCBI
12	Bunea A, Rugina D, Sconta Z, et al: Anthocyanin determination in blueberry extracts from various cultivars and their antiproliferative and apoptotic properties in B16-F10 metastatic murine melanoma cells. Phytochemistry. 95:436–444. 2013. View Article : Google Scholar
13	Fernandes I, Marques F, de Freitas V and Mateus N: Antioxidant and antiproliferative properties of methylated metabolites of anthocyanins. Food Chem. 141:2923–2933. 2013. View Article : Google Scholar : PubMed/NCBI
14	Heinonen M: Antioxidant activity and antimicrobial effect of berry phenolics - a Finnish perspective. Mol Nutr Food Res. 51:684–691. 2007. View Article : Google Scholar : PubMed/NCBI
15	Iriti M and Varoni EM: Chemopreventive potential of flavonoids in oral squamous cell carcinoma in human studies. Nutrients. 5:2564–2576. 2013. View Article : Google Scholar : PubMed/NCBI
16	Kamenickova A, Anzenbacherova E, Pavek P, et al: Effects of anthocyanins on the AhR-CYP1A1 signaling pathway in human hepatocytes and human cancer cell lines. Toxicol Lett. 21:1–8. 2013. View Article : Google Scholar
17	Chen PN, Chu SC, Chiou HL, Kuo WH, Chiang CL and Hsieh YS: Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Lett. 235:248–259. 2006. View Article : Google Scholar : PubMed/NCBI
18	Chen PN, Chu SC, Chiou HL, Chiang CL, Yang SF and Hsieh YS: Cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr Cancer. 53:232–243. 2005. View Article : Google Scholar : PubMed/NCBI
19	Ding M, Feng R, Wang SY, et al: Cyanidin-3-glucoside, a natural product derived from blackberry, exhibits chemopreventive and chemotherapeutic activity. J Biol Chem. 281:17359–17368. 2006. View Article : Google Scholar : PubMed/NCBI
20	Cooke D, Schwarz M, Boocock D, et al: Effect of cyanidin-3-glucoside and an anthocyanin mixture from bilberry on adenoma development in the ApcMin mouse model of intestinal carcinogenesis - relationship with tissue anthocyanin levels. Int J cancer. 119:2213–2220. 2006. View Article : Google Scholar

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Anthocyanins potentiate the activity of trastuzumab in human epidermal growth factor receptor 2‑positive breast cancer cells in vitro and in vivo

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles