Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2014 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2014 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells

  • Authors:
    • Weimin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
  • Pages: 2701-2705
    |
    Published online on: August 26, 2014
       https://doi.org/10.3892/mmr.2014.2518
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the effect of adenovirus‑mediated urokinase‑type plasminogen activator (uPA) transduction on uPA expression and fibrinolytic activity in human umbilical vein endothelial cells (HUVECs). Recombinant adenovirus vectors containing the human uPA gene were constructed and transduced into HUVECs. The expression and fibrinolytic activity of uPA was then assessed in HUVECs using western blot analysis, ELISA and colorimetric assay. The experiments were performed in three groups: The ad/uPa (n=3), ad/neg control (n=3) and blank control (n=3) groups. Western blot analysis revealed that uPA protein expression in the HUVECs in the ad/uPa group was significantly increased compared with those in the ad/neg control or blank groups (P<0.01). The uPA protein levels in the supernatant of the three groups were 379.40±2.46, 240.01±1.16 and 256.10±3.04 ng/l, respectively, showing that the uPA protein levels were significantly higher in the supernatant in the ad/uPa group compared with those in the ad/neg control or blank groups. uPA activity was determined using a colorimetric method and was found to be 40238.49±5755 IU/mg in the HUVECs in the ad/uPa group, which was significantly higher than that in the HUVECs in the ad/neg control (6180.03±942.38 IU/mg) or blank groups (3346.06±928.81 IU/mg) (both P<0.01). These findings suggested that transduction of the uPA gene increased uPA protein expression and fibrinolytic activity in HUVECs.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Goldhaber SZ: Pulmonary embolism. Lancet. 363:1295–1305. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Spencer FA, Emery C, Lessard D, et al: The Worcester Venous Thromboembolism Study: A population based study of the clinical epidemiology of venous thromboembolism. J Gen Intern Med. 21:722–727. 2006. View Article : Google Scholar

3 

Chapman NH, Brighton T, Harris MF, Caplan GA, Braithwaite J and Chong BH: Venous thromboembolism - management in general practice. Aust Fam Physician. 38:36–40. 2009.PubMed/NCBI

4 

Pengo V, Lensing AW, Prins MH, et al: Thromboembolic Pulmonary Hypertension Study Group: Incidence of chronic thromboembolic pulmonary hypertension after pulmonary embolism. N Engl J Med. 350:2257–2264. 2004. View Article : Google Scholar

5 

Vincenza Carriero M, Franco P, Vocca I, et al: Structure, function and antagonists of urokinase-type plasminogen activator. Front Biosci (Landmark Ed). 14:3782–3794. 2009.PubMed/NCBI

6 

Radha KS, Madhyastha HK, Nakajima Y, Omura S and Maruyama M: Emodin upregulates urokinase plasminogen activator, plasminogen activator inhibitor-1 and promotes wound healing in human fibroblasts. Vascul Pharmacol. 48:184–190. 2008. View Article : Google Scholar

7 

Nassar T, Yarovoi S, Fanne RA, et al: Urokinase plasminogen activator regulates pulmonary arterial contractility and vascular permeability in mice. Am J Respir Cell Mol Biol. 45:1015–1021. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Baldwin JF, Sood V, Elfline MA, et al: The role of urokinase plasminogen activator and plasmin activator inhibitor-1 on vein wall remodeling in experimental deep vein thrombosis. J Vasc Surg. 56:1089–1097. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Rao Gogineni V, Kumar Nalla A, Gupta R, et al: Radiation-inducible silencing of uPA and uPAR in vitro and in vivo in meningioma. Int J Oncol. 36:809–816. 2010.PubMed/NCBI

10 

Ylä-Herttuala S: Cardiovascular gene therapy with vascular endothelial growth factors. Gene. 525:217–219. 2013.

11 

Zhao B, Li X, Dai X and Gong N: Adenovirus-mediated anti-sense extracellular signal-regulated kinase 2 gene therapy inhibits activation of vascular smooth muscle cells and angiogenesis, and ameliorates transplant arteriosclerosis. Transplant Proc. 45:639–642. 2013. View Article : Google Scholar

12 

Dragneva G, Korpisalo P and Ylä-Herttuala S: Promoting blood vessel growth in ischemic diseases: challenges in translating preclinical potential into clinical success. Dis Model Mech. 6:312–322. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Kral BG and Kraitchman DL: From mice to men: gene therapy’s future for treatment of myocardial infarction. Circ Cardiovasc Imaging. 6:360–362. 2013.

14 

Botkjaer KA, Fogh S, Bekes EC, et al: Targeting the autolysis loop of urokinase-type plasminogen activator with conformation-specific monoclonal antibodies. Biochem J. 438:39–51. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Sood V, Luke CE, Deatrick KB, et al: Urokinase plasminogen activator independent early experimental thrombus resolution: MMP2 as an alternative mechanism. Thromb Haemost. 104:1174–1183. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Humphries J, Gossage JA, Modarai B, et al: Monocyte urokinase-type plasminogen activator up-regulation reduces thrombus size in a model of venous thrombosis. J Vasc Surg. 50:1127–1134. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Madhyastha R, Madhyastha H, Nakajima Y, Omura S and Maruyama M: Curcumin facilitates fibrinolysis and cellular migration during wound healing by modulating urokinase plasminogen activator expression. Pathophysiol Haemost Thromb. 37:59–66. 2010. View Article : Google Scholar

18 

Shenkman B, Livnat T, Budnik I, Tamarin I, Einav Y and Martinowitz U: Plasma tissue-type plasminogen activator increases fibrinolytic activity of exogenous urokinase-type plasminogen activator. Blood Coagul Fibrinolysis. 23:729–733. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Lipskaia L, Hadri L, Lopez JJ, Hajjar RJ and Bobe R: Benefit of SERCA2a gene transfer to vascular endothelial and smooth muscle cells: a new aspect in therapy of cardiovascular diseases. Curr Vasc Pharmacol. 11:465–479. 2013. View Article : Google Scholar

20 

Hennessy EJ and Moore KJ: Using microRNA as an alternative treatment for hyperlipidemia and cardiovascular disease: cardio-miRs in the pipeline. J Cardiovasc Pharmacol. 62:247–254. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Won YW, McGinn AN, Lee M, Nam K, Bull DA and Kim SW: Post-translational regulation of a hypoxia-responsive VEGF plasmid for the treatment of myocardial ischemia. Biomaterials. 34:6229–6238. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Yamaguchi Y, Yamada K, Suzuki T, et al: Induction of uPA release in human peripheral blood lymphocytes by [deamino-Cysl, D-Arg8]-vasopressin (dDAVP). Am J Physiol Endocrinol Metab. 287:E970–E976. 2004.

23 

De Meyer SF, Deckmyn H and Vanhoorelbeke K: von Willebrand factor to the rescue. Blood. 113:5049–5057. 2009.PubMed/NCBI

24 

Chey S, Claus C and Liebert UG: Validation and application of normalization factors for gene expression studies in rubella virus-infected cell lines with quantitative real-time PCR. J Cell Biochem. 110:118–128. 2010.PubMed/NCBI

25 

Gu Z, Pan J, Bankowski MJ and Hayden RT: Quantitative real-time polymerase chain reaction detection of BK virus using labeled primers. Arch Pathol Lab Med. 134:444–448. 2010.PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001.

27 

Oliveira MS, Skinner F, Arshadmansab MF, et al: Altered expression and function of small-conductance (SK) Ca(2+)-activated K+ channels in pilocarpine-treated epileptic rats. Brain Res. 1348:187–199. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Liang F, Arora N, Zhang KL, et al: A new, multiplex, quantitative real-time polymerase chain reaction system for nucleic Acid detection and quantification. Methods Mol Biol. 1039:51–68. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Wagner EM: Monitoring gene expression: quantitative real-time rt-PCR. Methods Mol Biol. 1027:19–45. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Gentilini F and Turba ME: Two novel real-time PCR methods for genotyping the von Willebrand disease type I mutation in Doberman Pinscher dogs. Vet J. 197:457–460. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou W: Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells. Mol Med Rep 10: 2701-2705, 2014.
APA
Zhou, W. (2014). Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells. Molecular Medicine Reports, 10, 2701-2705. https://doi.org/10.3892/mmr.2014.2518
MLA
Zhou, W."Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells". Molecular Medicine Reports 10.5 (2014): 2701-2705.
Chicago
Zhou, W."Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells". Molecular Medicine Reports 10, no. 5 (2014): 2701-2705. https://doi.org/10.3892/mmr.2014.2518
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou W: Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells. Mol Med Rep 10: 2701-2705, 2014.
APA
Zhou, W. (2014). Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells. Molecular Medicine Reports, 10, 2701-2705. https://doi.org/10.3892/mmr.2014.2518
MLA
Zhou, W."Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells". Molecular Medicine Reports 10.5 (2014): 2701-2705.
Chicago
Zhou, W."Effect of adenovirus‑mediated uPA gene transduction on the fibrinolytic activity of human umbilical vein endothelial cells". Molecular Medicine Reports 10, no. 5 (2014): 2701-2705. https://doi.org/10.3892/mmr.2014.2518
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team