MicroRNA-16 suppresses epithelial-mesenchymal transition‑related gene expression in human glioma

Wang,Qin; Li,Xu; Zhu,Yu; Yang,Ping

doi:10.3892/mmr.2014.2583

December-2014 Volume 10 Issue 6

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December-2014 Volume 10 Issue 6

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Article

MicroRNA-16 suppresses epithelial-mesenchymal transition‑related gene expression in human glioma

Authors:
- Qin Wang
- Xu Li
- Yu Zhu
- Ping Yang
View Affiliations / Copyright

Affiliations: Department of Clinical Laboratory, Tianjin Huanhu Hospital, Tianjin 300060, P.R. China
Pages: 3310-3314
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Published online on: September 19, 2014

https://doi.org/10.3892/mmr.2014.2583
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Abstract

Glioma is one of the most prevalent types of brain tumor and is associated with the highest mortality rate of all CNS cancers. Epithelial‑mesenchymal transition (EMT) has been recognized as an important factor in tumor metastasis. Previously, it has been demonstrated that microRNA-16 (miR-16) has an important role in tumor metastasis in human cancer cell lines. However, the role of miR-16 in epithelial‑mesenchymal transition of human glioma cells remains unclear. In the present study, U87 and U251 glioma cell lines overexpressing miR-16 were established and it was identified that miR-16 suppressed invasion, adhesion, cell cycle, production of interleukin (IL)-6, IL-8 and transforming growth factor-β, and EMT-related gene expression, including vimentin, β-catenin and E-cadherin in miR-16 overexpressing U87 and U251 glioma cells. Furthermore, miR-16 suppressed EMT mainly through the downregulation of p-FAK and p-Akt expression, and nuclear factor-κB and Slug transcriptional activity. Therefore, miR-16 may be an important therapeutic target and predictor for glioma therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA-16 suppresses epithelial-mesenchymal transition‑related gene expression in human glioma

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