Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 11 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro

  • Authors:
    • Wengang Yang
    • Hui Zheng
    • Yongyi Wang
    • Feng Lian
    • Zhenglei Hu
    • Song Xue
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 133-142
    |
    Published online on: October 23, 2014
       https://doi.org/10.3892/mmr.2014.2754
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the expression of nesprin‑1 protein in MSCs and its effects on the differentiation of rat bone‑marrow mesenchymal stem cells (MSCs). Surface‑associated antigens of MSCs were detected by flow cytometry. MSC differentiation was induced by treatment with 10 µmol/l 5‑azacytidine. Sprague‑Dawley rats were anesthetized prior to thoracotomy and subsequent ligation of the left anterior descending coronary artery to establish a model of myocardial infarction. Two weeks following myocardial infarction, DAPI‑marked MSCs were injected into the infarcted region in the experimental group, while DMEM was injected into the infarcted region of the control group. Characteristics of the putative cardiac‑myogenic cells were evaluated using immunohistochemical and immunofluorescent analysis. The messenger RNA expression levels of cardiac‑myogenic specific genes; desmin, α‑actinin and cardiac troponin I (cTnI) were detected by reverse transcription quantitative polymerase chain reaction. The expression of nesprin‑1 protein in MSCs was identified by immunofluorescence and western blot analysis, prior to and following MSC differentiation. Following differentiation, the MSCs appeared spindle‑shaped with irregular processes and were positive for CD90 and CD29, but negative for CD45. Cardiomyocyte‑like cells were positive for desmin, α‑sarcomeric actin and cTnI. Nesprin protein was detected in the nuclear membrane via immunofluorescence, and following MSC differentiation into cardiomyocyte‑like cells, the expression of nesprin protein was significantly higher (*P=0.03<0.05). The results of the present study indicated that MSCs may be differentiated in vitro and in vivo into cells with characteristics commonly attributed to cardiomyocytes. Cardiomyocyte‑like cells cultured from bone marrow sources may be potentially useful for repairing the injured myocardium. The results also suggested that, consistent with the results of previous studies, the expression of nesprin‑1 protein was higher during the differentiation process of MSCs and may have an important role in mediating MSC differentiation. Elucidation of the role of nesprin‑1 in MSC differentiation may aid in the development of novel therapies for the treatment of myocardial ischemia and nesprin‑1 genetic deficiencies.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Zhang Q, Skepper JN, Yang F, et al: Nesprins: a novel family of spectrin-repeat-containing proteins that localize to the nuclear membrane in multiple tissues. J Cell Sci. 114:4485–4498. 2001.PubMed/NCBI

2 

Apel ED, Lewis RM, Grady RM and Sanes JR: Syne-1, a dystrophin- and Klarsicht-related protein associated with synaptic nuclei at the neuromuscular junction. J Biol Chem. 275:31986–31995. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Lammerding J, Schulze PC, Takahashi T, et al: Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J Clin Invest. 113:370–378. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Zhang Q, Ragnauth CD, Skepper JN, et al: Nesprin-2 is a multi-isomeric protein that binds lamin and emerin at the nuclear envelope and forms a subcellular network in skeletal muscle. J Cell Sci. 118(Pt 4): 673–687. 2005. View Article : Google Scholar

5 

Pittenger MF, Mackay AM, Beck SC, et al: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Minguell JJ, Erices A and Conget P: Mesenchymal stem cells. Exp Biol Med (Maywood). 226:507–520. 2001.PubMed/NCBI

7 

Devine SM: Mesenchymal stem cells: will they have a role in the clinic? J Cell Biochem Suppl. 38:73–79. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Nagaya N, Fujii T, Iwase T, et al: Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis. Am J Physiol Heart Circ Physiol. 287:H2670–H2676. 2004. View Article : Google Scholar

9 

Miyahara Y, Nagaya N, Kataoka M, et al: Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nat Med. 12:459–465. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Nagaya N, Kangawa K, Itoh T, et al: Transplantation of mesenchymal stem cells improves cardiac function in a rat model of dilated cardiomyopathy. Circulation. 112:1128–1135. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Chedrawy EG, Wang JS, Nguyen DM, et al: Incorporation and integration of implanted myogenic and stem cells into native myocardial fibers: anatomic basis for functional improvements. J Thorac Cardiovasc Surg. 124:584–590. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Min JY, Sandmann S, Meissner A, et al: Differential effects of mibefradil, verapamil, and amlodipine on myocardial function and intracellular Ca(2+) handling in rats with chronic myocardial infarction. J Pharmacol Exp Ther. 291:1038–1044. 1999.PubMed/NCBI

13 

Chomczynski P and Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:156–159. 1987. View Article : Google Scholar : PubMed/NCBI

14 

Martina JD1, Simmons C and Jukic DM: High-definition hematoxylin and eosin staining in a transition to digital pathology. J Pathol Inform. 2:452011. View Article : Google Scholar : PubMed/NCBI

15 

Friedenstein AJ, Petrakova KV, Kurolesova AI, et al: Heterotopic transplants of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation. 6:230–247. 1968. View Article : Google Scholar : PubMed/NCBI

16 

Dennis JE and Charbord P: Origin and differentiation of human and murine stroma. Stem Cells. 20:205–214. 2002. View Article : Google Scholar : PubMed/NCBI

17 

Campagnoli C, Roberts IA, Kumar S, et al: Identification of mesenchymal stem/progenitor cells in human first-trimester fetal blood, liver, and bone marrow. Blood. 98:2396–2402. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Martin DR, Cox NR, Hathcock TL, et al: Isolation and characterization of multipotential mesenchymal stem cells from feline bone marrow. Exp Hematol. 30:879–886. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Wakitani S, Saito T and Caplan AI: Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve. 18:1417–1426. 1995. View Article : Google Scholar : PubMed/NCBI

20 

Dominici M, Le Blanc K, Mueller I, et al: Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 8:315–317. 2006. View Article : Google Scholar

21 

Xu W, Zhang X, Qian H, et al: Mesenchymal stem cells from adult human bone marrow differentiate into a cardiomyocyte phenotype in vitro. Exp Biol Med (Maywood). 229:623–631. 2004.PubMed/NCBI

22 

Makino S, Fukuda K, Miyoshi S, et al: Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest. 103:697–705. 1999. View Article : Google Scholar : PubMed/NCBI

23 

Toma C, Pittenger MF, Cahill KS, et al: Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation. 105:93–98. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Yoon J, Min BG, Kim YH, et al: Differentiation, engraftment and functional effects of pre-treated mesenchymal stem cells in a rat myocardial infarct model. Acta Cardiol. 60:277–284. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Xie XJ, Wang JA, Cao J and Zhong X: Differentiation of bone marrow mesenchymal stem cells induced by myocardial medium under hypoxic conditions. Acta Pharmacol Sin. 27:1153–1158. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Forte G, Minieri M, Cossa P, et al: Hepatocyte growth factor effects on mesenchymal stem cells: proliferation, migration, and differentiation. Stem Cells. 24:23–33. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Li W, Ma N, Ong LL, et al: Bcl-2 engineered MSCs inhibited apoptosis and improved heart function. Stem Cells. 25:2118–2127. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Hu X, Wang J, Chen J, et al: Optimal temporal delivery of bone marrow mesenchymal stem cells in rats with myocardial infarction. Eur J Cardiothorac Surg. 31:438–443. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Mislow JM, Holaska JM, Kim MS, et al: Nesprin-1alpha self-associates and binds directly to emerin and lamin A in vitro. FEBS Lett. 525:135–140. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Mislow JM, Kim MS, Davis DB, et al: Myne-1, a spectrin repeat transmembrane protein of the myocyte inner nuclear membrane, interacts with lamin A/C. J Cell Sci. 115(Pt 1): 61–70. 2002.PubMed/NCBI

31 

Starr DA and Han M: Role of ANC-1 in tethering nuclei to the actin cytoskeleton. Science. 298:406–409. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Starr DA and Han M: ANChors away: an actin based mechanism of nuclear positioning. J Cell Sci. 116(Pt 2): 211–216. 2003. View Article : Google Scholar : PubMed/NCBI

33 

Gough LL, Fan J, Chu S, et al: Golgi localization of Syne-1. Mol Biol Cell. 14:2410–2424. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Zhang Q, Ragnauth C, Greener MJ, et al: The nesprins are giant actin-binding proteins, orthologous to Drosophila melanogaster muscle protein MSP-300. Genomics. 80:473–481. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Pare GC, Easlick JL, Mislow JM, et al: Nesprin-1alpha contributes to the targeting of mAKAP to the cardiac myocyte nuclear envelope. Exp Cell Res. 303:388–399. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Puckelwartz MJ, Kessler EJ, Kim G, et al: Nesprin-1 mutations in human and murine cardiomyopathy. J Mol Cell Cardiol. 48:600–608. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Randles KN, Lam le T, Sewry CA, et al: Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development. Dev Dyn. 239:998–1009. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang W, Zheng H, Wang Y, Lian F, Hu Z and Xue S: Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro. Mol Med Rep 11: 133-142, 2015.
APA
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., & Xue, S. (2015). Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro. Molecular Medicine Reports, 11, 133-142. https://doi.org/10.3892/mmr.2014.2754
MLA
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., Xue, S."Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro". Molecular Medicine Reports 11.1 (2015): 133-142.
Chicago
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., Xue, S."Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro". Molecular Medicine Reports 11, no. 1 (2015): 133-142. https://doi.org/10.3892/mmr.2014.2754
Copy and paste a formatted citation
x
Spandidos Publications style
Yang W, Zheng H, Wang Y, Lian F, Hu Z and Xue S: Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro. Mol Med Rep 11: 133-142, 2015.
APA
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., & Xue, S. (2015). Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro. Molecular Medicine Reports, 11, 133-142. https://doi.org/10.3892/mmr.2014.2754
MLA
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., Xue, S."Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro". Molecular Medicine Reports 11.1 (2015): 133-142.
Chicago
Yang, W., Zheng, H., Wang, Y., Lian, F., Hu, Z., Xue, S."Nesprin‑1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte‑like cells in vivo and in vitro". Molecular Medicine Reports 11, no. 1 (2015): 133-142. https://doi.org/10.3892/mmr.2014.2754
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team