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Article

Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation

  • Authors:
    • Duo Zhang
    • Bin Chen
    • Jian Zhou
    • Lin Zhou
    • Qing Li
    • Fei Liu
    • Kuang‑Yen Chou
    • Lei Tao
    • Li‑Ming Lu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University School of Medicine, Shanghai 200031, P.R. China, Shanghai Institute of Immunology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 349-356
    |
    Published online on: October 23, 2014
       https://doi.org/10.3892/mmr.2014.2760
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Abstract

Trichosanthin (TCS) is a type I ribosome‑­inactivating protein, which inhibits cell viability in human epithelial type 2 (HEp‑2) and AMC‑HN‑8 human laryngeal epidermoid carcinoma cells. Although TCS is a potential chemotherapeutic agent, its mechanism of action remains to be elucidated. In the present study, HEp‑2 and AMC‑HN‑8 cells were treated with different concentrations of TCS combined with or without cisplatin. After 5 days of successive treatment, different experimental groups were detected using a cell counting kit‑8 and the collected supernatants were analyzed using a lactate dehydrogenase kit. Flow cytometric assays were performed to detect apoptosis and cell cycle arrest in the HEp‑2 and AMC‑HN‑8 cells, reverse transcription quantitative polymerase chain reaction was performed to detect the levels of p27, p21WAF and western blot analysis was performed to detect changes in c‑Jun N‑terminal protein kinase (JNK)/phosphorylated (phospho)‑JNK, p38/phospho‑p38, extracellular signal‑regulated kinase (ERK)/phospho‑ERK, caspase‑3 and caspase‑9 in the HEp‑2 and AMC‑HN‑8 cancer cells. TCS significantly inhibited the cell viability of the HEp‑2 and AMC‑HN‑8 cells, independently of necrosis. TCS induced apoptosis and increased the percentage of HEp‑2 and AMC‑HN‑8 cells in the S‑phase of the cell cycle. In addition, the JNK/mitogen‑activated protein kinase (MAPK) pathway was activated by TCS in the HEp‑2 and AMC‑HN‑8 cells. Low concentrations of TCS also induced apoptosis and S‑phase cell cycle arrest in the HEp‑2 and AMC‑HN‑8 cells. The antitumor effects of TCS may be associated with JNK/MAPK activation
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1 

Shaw PC, Chan WL, Yeung HW and Ng TB: Minireview: trichosanthin - a protein with multiple pharmacological properties. Life Sci. 55:253–262. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Li M, Li X and Li JC: Possible mechanisms of trichosanthin-induced apoptosis of tumor cells. Anat Rec (Hoboken). 293:986–992. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Tsao SW, Ng TB and Yeung HW: Toxicities of trichosanthin and alpha-momorcharin, abortifacient proteins from Chinese medicinal plants, on cultured tumor cell lines. Toxicon. 28:1183–1192. 1990. View Article : Google Scholar : PubMed/NCBI

4 

Fang EF, Ng TB, Shaw PC and Wong RN: Recent progress in medicinal investigations on trichosanthin and other ribosome inactivating proteins from the plant genus Trichosanthes. Curr Med Chem. 18:4410–4417. 2011. View Article : Google Scholar : PubMed/NCBI

5 

He D, Yau K, He X, et al: Conversion of trichosanthin-induced CD95 (Fas) type I into type II apoptotic signaling during Herpes simplex virus infection. Mol Immunol. 48:2000–2008. 2011. View Article : Google Scholar : PubMed/NCBI

6 

He D, Zheng Y and Tam S: The anti-herpetic activity of trichosanthin via the nuclear factor-kappaB and p53 pathways. Life Sci. 90:673–681. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Sha O, Yew DT, Ng TB, Yuan L and Kwong WH: Different in vitro toxicities of structurally similar type I ribosome-inactivating proteins (RIPs). Toxicol In Vitro. 24:1176–1182. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Wagner EF and Nebreda AR: Signal integration by JNK and p38 MAPK pathways in cancer development. Nat Rev Cancer. 9:537–549. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Wang P, Xu J and Zhang C: CREB, a possible upstream regulator of Bcl-2 in trichosanthin-induced HeLa cell apoptosis. Mol Biol Rep. 37:1891–1896. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Wang P and Li JC: Trichosanthin-induced specific changes of cytoskeleton configuration were associated with the decreased expression level of actin and tubulin genes in apoptotic Hela cells. Life Sci. 81:1130–1140. 2007. View Article : Google Scholar

11 

Liu J and Lin A: Role of JNK activation in apoptosis: a double-edged sword. Cell Res. 15:36–42. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Ouyang DY, Chan H, Wang YY, et al: An inhibitor of c-Jun N-terminal kinases (CEP-11004) counteracts the anti-HIV-1 action of trichosanthin. Biochem Biophys Res Commun. 339:25–29. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Huang H, Chan H, Wang YY, et al: Trichosanthin suppresses the elevation of p38 MAPK, and Bcl-2 induced by HSV-1 infection in Vero cells. Life Sci. 79:1287–1292. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Wang P, Chen LL, Yan H and Li JC: Trichosanthin suppresses HeLa cell proliferation through inhibition of the PKC/MAPK signaling pathway. Cell Biol Toxicol. 25:479–488. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Kanduc D, Mittelman A, Serpico R, et al: Cell death: apoptosis versus necrosis (review). Int J Oncol. 21:165–170. 2002.PubMed/NCBI

16 

Gao LL, Feng L, Yao ST, et al: Molecular mechanisms of celery seed extract induced apoptosis via s phase cell cycle arrest in the BGC-823 human stomach cancer cell line. Asian Pac J Cancer Prev. 12:2601–2606. 2011.PubMed/NCBI

17 

Chirgwin JM, Przybyla AE, MacDonald RJ and Rutter WJ: Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 18:5294–5299. 1979.PubMed/NCBI

18 

Perez IC, Le Guiner C, Ni W, Lyles J, Moullier P and Snyder RO: PCR-based detection of gene transfer vectors: application to gene doping surveillance. Anal Bioanal Chem. 405:9641–9653. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Korzeniewski C and Callewaert DM: An enzyme-release assay for natural cytotoxicity. J Immunol Methods. 64:313–320. 1983. View Article : Google Scholar : PubMed/NCBI

20 

Decker T and Lohmann-Matthes ML: A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods. 115:61–69. 1988. View Article : Google Scholar

21 

Lu Z and Hunter T: Ubiquitylation and proteasomal degradation of the p21(Cip1), p27(Kip1) and p57(Kip2) CDK inhibitors. Cell Cycle. 9:2342–2352. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Bennett BL, Sasaki DT, Murray BW, et al: SP600125, an anthrapyrazolone inhibitor of Jun N-terminal kinase. Proc Natl Acad Sci USA. 98:13681–13686. 2001.PubMed/NCBI

23 

Xu J, Gao DF, Yan GL and Fan JM: Induced apoptotic action of recombinant trichosanthin in human stomach adenocarcinoma MCG803 cells. Mol Biol Rep. 36:1559–1564. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Lefebvre JL: Candidates for larynx preservation: the next step? Oncologist. 15(Suppl 3): 30–32. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Chen T, Stephens PA, Middleton FK and Curtin NJ: Targeting the S and G2 checkpoint to treat cancer. Drug Discov Today. 17:194–202. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Miotto B and Struhl K: JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress. Mol Cell. 44:62–71. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Li J, Xia X, Nie H, Smith MA and Zhu X: PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562. Biochim Biophys Acta. 1770:63–70. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Li J, Xia X, Ke Y, et al: Trichosanthin induced apoptosis in HL-60 cells via mitochondrial and endoplasmic reticulum stress signaling pathways. Biochim Biophys Acta. 1770:1169–1180. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Wang P, Yan H and Li JC: CREB-mediated Bcl-2 expression in trichosanthin-induced Hela cell apoptosis. Biochem Biophys Res Commun. 363:101–105. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Zhang C, Gong Y, Ma H, et al: Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells. Biochem J. 355:653–661. 2001.PubMed/NCBI

31 

Li M, Chen F, Liu CP, et al: Dexamethasone enhances trichosanthin-induced apoptosis in the HepG2 hepatoma cell line. Life Sci. 86:10–16. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang D, Chen B, Zhou J, Zhou L, Li Q, Liu F, Chou KY, Tao L and Lu LM: Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation. Mol Med Rep 11: 349-356, 2015.
APA
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F. ... Lu, L. (2015). Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation. Molecular Medicine Reports, 11, 349-356. https://doi.org/10.3892/mmr.2014.2760
MLA
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F., Chou, K., Tao, L., Lu, L."Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation". Molecular Medicine Reports 11.1 (2015): 349-356.
Chicago
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F., Chou, K., Tao, L., Lu, L."Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation". Molecular Medicine Reports 11, no. 1 (2015): 349-356. https://doi.org/10.3892/mmr.2014.2760
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang D, Chen B, Zhou J, Zhou L, Li Q, Liu F, Chou KY, Tao L and Lu LM: Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation. Mol Med Rep 11: 349-356, 2015.
APA
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F. ... Lu, L. (2015). Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation. Molecular Medicine Reports, 11, 349-356. https://doi.org/10.3892/mmr.2014.2760
MLA
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F., Chou, K., Tao, L., Lu, L."Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation". Molecular Medicine Reports 11.1 (2015): 349-356.
Chicago
Zhang, D., Chen, B., Zhou, J., Zhou, L., Li, Q., Liu, F., Chou, K., Tao, L., Lu, L."Low concentrations of trichosanthin induce apoptosis and cell cycle arrest via c‑Jun N‑terminal protein kinase/mitogen‑activated protein kinase activation". Molecular Medicine Reports 11, no. 1 (2015): 349-356. https://doi.org/10.3892/mmr.2014.2760
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