Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2015 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 11 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Visfatin: An adipokine activator of rat hepatic stellate cells

  • Authors:
    • Ning‑Lin Liang
    • Ruoting Men
    • Yongjun Zhu
    • Cong Yuan
    • Yan Wei
    • Xiaojing Liu
    • Li Yang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Cardiovascular Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1073-1078
    |
    Published online on: October 29, 2014
       https://doi.org/10.3892/mmr.2014.2795
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study was conducted to investigate the effects of visfatin on the activation of hepatic stellate cells (HSC) and the possible underlying mechanism. HSC were isolated from the livers of Sprague‑Dawley rats by in situ perfusion of collagenase and pronase and a single‑step density Nycodenz gradient. The culture‑activated cells were serum‑starved and incubated with different concentrations of recombinant visfatin (0, 25, 50, 100 or 200 ng/ml) for 24 h. The expression of α‑smooth muscle actin (α‑SMA), collagen types I and III and connective tissue growth factor (CTGF) were then measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis. The results demonstrated that 100 and 200 ng/ml concentrations of visfatin induced the expression of α‑SMA in culture‑activated rat HSC, which was accompanied by a significant increase in collagen types I and III, as confirmed by western blot and RT‑qPCR analyses. In addition, treatment of the HSC with certain concentrations of visfatin upregulated the expression of CTGF. These findings suggested that visfatin activated HSC and induced the production of collagen types I and III.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Bertolani C and Marra F: The role of adipokines in liver fibrosis. Pathophysiology. 15:91–101. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Wu D, Li H, Xiang G, et al: Adiponectin and its receptors in chronic hepatitis B patients with steatosis in china. Hepat Mon. 13:e60652013. View Article : Google Scholar : PubMed/NCBI

3 

Tsochatzis EA, Papatheodoridis GV and Archimandritis AJ: Adipokines in nonalcoholic steatohepatitis: from pathogenesis to implications in diagnosis and therapy. Mediators Inflamm. 2009:8316702009. View Article : Google Scholar : PubMed/NCBI

4 

Marra F and Bertolani C: Adipokines in liver diseases. Hepatology. 50:957–969. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Romacho T, Villalobos LA, Cercas E, Carraro R, Sanchez-Ferrer CF and Peiró C: Visfatin as a novel mediator released by inflamed human endothelial cells. PLoS One. 8:e782832013. View Article : Google Scholar : PubMed/NCBI

6 

Van den Bergh R, Morin S, Sass HJ, et al: Monocytes contribute to differential immune pressure on R5 versus X4 HIV through the adipocytokine visfatin/NAMPT. PLoS One. 7:e350742012. View Article : Google Scholar : PubMed/NCBI

7 

Yu XY, Qiao SB, Guan HS, Liu SW and Meng XM: Effects of visfatin on proliferation and collagen synthesis in rat cardiac fibroblasts. Horm Metab Res. 42:507–513. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Wang P, Xu TY, Guan YF, Su DF, Fan GR and Miao CY: Perivascular adipose tissue-derived visfatin is a vascular smooth muscle cell growth factor: role of nicotinamide mononucleotide. Cardiovasc Res. 81:370–380. 2009. View Article : Google Scholar

9 

Song HK, Lee MH, Kim BK, et al: Visfatin: a new player in mesangial cell physiology and diabetic nephropathy. Am J Physiol Renal Physiol. 295:F1485–F1494. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Liu XJ, Yang L, Mao YQ, et al: Effects of the tyrosine protein kinase inhibitor genistein on the proliferation, activation of cultured rat hepatic stellate cells. World J Gastroenterol. 8:739–745. 2002.PubMed/NCBI

11 

Friedman SL: Hepatic stellate cells: protean, multifunctional, and enigmatic cells of the liver. Physiol Rev. 88:125–172. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Nieto N: A systems biology approach for understanding the collagen regulatory network in alcoholic liver disease. Liver Int. 32:189–198. 2012. View Article : Google Scholar

13 

Zhu Y, Men R, Wen M, Hu X, Liu X and Yang L: Blockage of TRPM7 channel induces hepatic stellate cell death through endoplasmic reticulum stress-mediated apoptosis. Life Sci. 94:37–44. 2014. View Article : Google Scholar

14 

Liu XJ, Yang L, Wu HB, Qiang O, Huang MH and Wang YP: Apoptosis of rat hepatic stellate cells induced by anti-focal adhesion kinase antibody. World J Gastroenterol. 8:734–738. 2002.PubMed/NCBI

15 

Kim JG, Kim EO, Jeong BR, et al: Visfatin stimulates proliferation of MCF-7 human breast cancer cells. Mol Cells. 30:341–345. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Patel ST, Mistry T, Brown JE, et al: A novel role for the adipokine visfatin/pre-B cell colony-enhancing factor 1 in prostate carcinogenesis. Peptides. 31:51–57. 2010. View Article : Google Scholar

17 

Höinghaus R, Hewicker-Trautwein M and Mischke R: Immunocytochemical differentiation of neoplastic and hyperplastic canine epithelial lesions in cytologic imprint preparations. Vet J. 173:79–90. 2007. View Article : Google Scholar

18 

Gilliver SC, Ruckshanthi JP, Atkinson SJ and Ashcroft GS: Androgens influence expression of matrix proteins and proteolytic factors during cutaneous wound healing. Lab Invest. 87:871–881. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Maeda E, Shelton JC, Bader DL and Lee DA: Differential regulation of gene expression in isolated tendon fascicles exposed to cyclic tensile strain in vitro. J Appl Physiol (1985). 106:506–512. 2009. View Article : Google Scholar

20 

Li L, Chen GP, Yang Y, Ye Y, Yao L and Hu SJ: Chronic inhibition of farnesyl pyrophosphate synthase attenuates cardiac hypertrophy and fibrosis in spontaneously hypertensive rats. Biochem Pharmacol. 79:399–406. 2010. View Article : Google Scholar

21 

Chen A and Zheng S: Curcumin inhibits connective tissue growth factor gene expression in activated hepatic stellate cells in vitro by blocking NF-kappaB and ERK signalling. Br J Pharmacol. 153:557–567. 2008. View Article : Google Scholar

22 

Dodig M, Ogunwale B, Dasarathy S, Li M, Wang BC and McCullough AJ: Differences in regulation of type I collagen synthesis in primary and passaged hepatic stellate cell cultures: the role of alpha5beta1-integrin. Am J Physiol Gastrointest Liver Physiol. 293:G154–G164. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Troeger JS, Mederacke I, Gwak GY, Dapito DH, Mu X, Hsu CC, Pradere JP, et al: Deactivation of hepatic stellate cells during liver fibrosis resolution in mice. Gastroenterology. 143:1073–1083. e10222012. View Article : Google Scholar : PubMed/NCBI

24 

Ponomarenko Y, Leo MA, Kroll W and Lieber CS: Effects of alcohol consumption on eight circulating markers of liver fibrosis. Alcohol and Alcoholism. 37:252–255. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Cheng F, Li Y, Feng L and Li S: Hepatic stellate cell activation and hepatic fibrosis induced by ischemia/reperfusion injury. Transplant Proc. 40:2167–2170. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Gressner OA, Lahme B, Demirci I, Gressner AM and Weiskirchen R: Differential effects of TGF-beta on connective tissue growth factor (CTGF/CCN2) expression in hepatic stellate cells and hepatocytes. J Hepatol. 47:699–710. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Jiang JX, Mikami K, Shah VH and Torok NJ: Leptin induces phagocytosis of apoptotic bodies by hepatic stellate cells via a Rho guanosine triphosphatase-dependent mechanism. Hepatology. 48:1497–1505. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Kamada Y, Tamura S, Kiso S, et al: Enhanced carbon tetrachloride-induced liver fibrosis in mice lacking adiponectin. Gastroenterology. 125:1796–1807. 2003. View Article : Google Scholar

29 

Adya R, Tan BK, Punn A, Chen J and Randeva HS: Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis. Cardiovasc Res. 78:356–365. 2008. View Article : Google Scholar

30 

Kim SR, Bae SK, Choi KS, et al: Visfatin promotes angiogenesis by activation of extracellular signal-regulated kinase 1/2. Biochem Biophys Res Commun. 357:150–156. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Xie H, Tang SY, Luo XH, et al: Insulin-like effects of visfatin on human osteoblasts. Calcif Tissue Int. 80:201–210. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Brenner DA: Molecular pathogenesis of liver fibrosis. Trans Am Clin Climatol Assoc. 120:361–368. 2009.PubMed/NCBI

33 

Liu Y, Liu H, Meyer C, Li J, Nadalin S, Konigsrainer A, Weng H, et al: Transforming growth factor-beta (TGF-beta)-mediated connective tissue growth factor (CTGF) expression in hepatic stellate cells requires Stat3 signaling activation. J Biol Chem. 288:30708–30719. 2013. View Article : Google Scholar : PubMed/NCBI

34 

Paradis V, Dargere D, Bonvoust F, Vidaud M, Segarini P and Bedossa P: Effects and regulation of connective tissue growth factor on hepatic stellate cells. Lab Invest. 82:767–773. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Leask A, Holmes A, Black CM and Abraham DJ: Connective tissue growth factor gene regulation. Requirements for its induction by transforming growth factor-beta 2 in fibroblasts. J Biol Chem. 278:13008–13015. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Zheng S and Chen A: Curcumin suppresses the expression of extracellular matrix genes in activated hepatic stellate cells by inhibiting gene expression of connective tissue growth factor. Am J Physiol Gastrointest Liver Physiol. 290:G883–G893. 2006. View Article : Google Scholar

37 

Li G, Xie Q, Shi Y, et al: Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. J Gene Med. 8:889–900. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Crean JK, Finlay D, Murphy M, et al: The role of p42/44 MAPK and protein kinase B in connective tissue growth factor induced extracellular matrix protein production, cell migration, and actin cytoskeletal rearrangement in human mesangial cells. J Biol Chem. 277:44187–44194. 2002. View Article : Google Scholar : PubMed/NCBI

39 

Gore-Hyer E, Pannu J, Smith EA, Grotendorst G and Trojanowska M: Selective stimulation of collagen synthesis in the presence of costimulatory insulin signaling by connective tissue growth factor in scleroderma fibroblasts. Arthritis Rheum. 48:798–806. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Kubota S and Takigawa M: Role of CCN2/CTGF/Hcs24 in bone growth. Int Rev Cytol. 257:1–41. 2007. View Article : Google Scholar : PubMed/NCBI

41 

Ivkovic S, Yoon BS, Popoff SN, et al: Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development. Development. 130:2779–2791. 2003. View Article : Google Scholar : PubMed/NCBI

42 

Shi-wen X, Stanton LA, Kennedy L, et al: CCN2 is necessary for adhesive responses to transforming growth factor-beta1 in embryonic fibroblasts. J Biol Chem. 281:10715–10726. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liang NL, Men R, Zhu Y, Yuan C, Wei Y, Liu X and Yang L: Visfatin: An adipokine activator of rat hepatic stellate cells. Mol Med Rep 11: 1073-1078, 2015.
APA
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., & Yang, L. (2015). Visfatin: An adipokine activator of rat hepatic stellate cells. Molecular Medicine Reports, 11, 1073-1078. https://doi.org/10.3892/mmr.2014.2795
MLA
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., Yang, L."Visfatin: An adipokine activator of rat hepatic stellate cells". Molecular Medicine Reports 11.2 (2015): 1073-1078.
Chicago
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., Yang, L."Visfatin: An adipokine activator of rat hepatic stellate cells". Molecular Medicine Reports 11, no. 2 (2015): 1073-1078. https://doi.org/10.3892/mmr.2014.2795
Copy and paste a formatted citation
x
Spandidos Publications style
Liang NL, Men R, Zhu Y, Yuan C, Wei Y, Liu X and Yang L: Visfatin: An adipokine activator of rat hepatic stellate cells. Mol Med Rep 11: 1073-1078, 2015.
APA
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., & Yang, L. (2015). Visfatin: An adipokine activator of rat hepatic stellate cells. Molecular Medicine Reports, 11, 1073-1078. https://doi.org/10.3892/mmr.2014.2795
MLA
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., Yang, L."Visfatin: An adipokine activator of rat hepatic stellate cells". Molecular Medicine Reports 11.2 (2015): 1073-1078.
Chicago
Liang, N., Men, R., Zhu, Y., Yuan, C., Wei, Y., Liu, X., Yang, L."Visfatin: An adipokine activator of rat hepatic stellate cells". Molecular Medicine Reports 11, no. 2 (2015): 1073-1078. https://doi.org/10.3892/mmr.2014.2795
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team