Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2015 Volume 11 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2015 Volume 11 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis

  • Authors:
    • Xiong Zhang
    • Dong‑Dong Qi
    • Ting‑Ting Zhang
    • Qing‑Xin Chen
    • Guang‑Zhi Wang
    • Guang‑Yu Sui
    • Xue‑Wei Hao
    • Shouli Sun
    • Xue Song
    • Ying‑Li Chen
  • View Affiliations / Copyright

    Affiliations: Department of Psychiatry, Hulunbeier Mental Health Center, Yakeshi, Inner Mongolia 022150, P.R. China, Clinical Laboratory, Hulunbeier Mental Health Center, Yakeshi, Inner Mongolia 022150, P.R. China, Department of Urinary Surgery, General Hospital of Daqing Oil Field, Daqing, Heilongjiang 163000, P.R. China, Department of Pharmacology, Harbin Medical University, Harbin, Heilongjiang 150000, P.R. China, Department of Pathology, The Fifth Affiliated Hospital, Harbin Medical University, Daqing, Heilongjiang 163319, P.R. China, Clinical Laboratory, Chinese Medicine Hospital of Daqing, Daqing, Heilongjiang 163319, P.R. China, Department of Immunology, College of Medical Laboratory Science and Technology, Harbin Medical University‑Daqing, Daqing, Heilongjiang 163319, P.R. China
  • Pages: 2083-2091
    |
    Published online on: November 10, 2014
       https://doi.org/10.3892/mmr.2014.2910
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The T42 peptide, generated from two active fragments of tumstatin, has been shown to have anti‑tumor activity. The adenoviral vector is the most frequently used vector in research and clinical trials for gene therapy. In the present study, the anti‑tumor activity of the T42 peptide and quadruple T42 (4xT42) peptide adenoviral vectors were elucidated for the first time, to the best of our knowledge. Human embryonic kidney 293 cells were infected with plasmid adenovirus (pAd)‑enhanced green fluorescent protein (EGFP)‑T42 or pAd‑EGFP‑4xT42 and the expression of the T42 and 4xT42 genes was confirmed by the identification of GFP expression and reverse transcription polymerase chain reaction experiments. The anti‑cancer effects of pAd‑EGFP‑T42 and pAd‑EGFP‑4xT42 on breast cancer cells in vivo and in vitro were subsequently investigated. The results indicated that the packaging of the recombinant adenoviruses with the viral titer was successful, following purification at 5x109 plaque forming units/ml. The results also revealed that the recombinant adenoviruses promoted apoptosis in MCF‑7 breast cancer cells and inhibited cancer growth. Through the analysis of caspase‑3, B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein expression, it was demonstrated that the T42/4xT42 peptide may induce apoptosis via the mitochondrial pathway. In addition, mouse xenograft experiments confirmed that the T42 peptide inhibited tumor growth and reduced angiogenesis in vivo. In conclusion, the results of the present study indicated that the T42 and 4xT42 peptide genes, transfected by a recombinant adenovirus, may provide a potential novel strategy for the treatment of breast cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Tazawa H, Kagawa S and Fujiwara T: Advances in adenovirus-mediated p53 cancer gene therapy. Expert Opin Biol Ther. 13:1569–1583. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Liu SX, Xia ZS and Zhong YQ: Gene therapy in pancreatic cancer. World J Gastroenterol. 20:13343–13368. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Folkman J: Tumour angiogenesis: therapeutic implications. N Engl J Med. 285:1182–1186. 1971. View Article : Google Scholar : PubMed/NCBI

4 

Hlatky L, Hahnfeldt P and Folkman J: Clinical application of antiangiogenic therapy: microvessel density, what it does and doesn’t tell us. J Natl Cancer Inst. 94:883–893. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Albig AR and Schiemann WP: Fibulin-5 antagonizes vascular endothelial growth factor (VEGF) signaling and angiogenic sprouting by endothelial cells. DNA Cell Biol. 23:367–379. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Sim BK: Angiostatin and endostatin: endothelial cell-specific endogenous inhibitors of angiogenesis and tumor growth. Angiogenesis. 2:37–48. 1998. View Article : Google Scholar

7 

Ribatti D: History of research on angiogenesis. Chem Immunol Allergy. 99:1–14. 2014. View Article : Google Scholar

8 

Hamano Y, Zeisberg M, Sugimoto H, et al: Physiological levels of tumstatin, a fragment of collagen IV alpha3 chain, are generated by MMP-9 proteolysis and suppress angiogenesis via alphaVbeta3 integrin. Cancer Cell. 3:589–601. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Su XJ, Wu FJ, Yuan LJ and Lin XS: Determination of anti-Hepg2 cells activity and purification of tumor chalone T42 peptide. J Jilin Univ, Med Ed. 36:86–89. 2010.

10 

Maeshima Y, Colorado PC, Torre A, et al: Distinct antitumor properties of a type IV collagen domain derived from basement membrane. J Biol Chem. 275:21340–21348. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Maeshima Y, Manfredi M, Reimer C, et al: Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin. J Biol Chem. 276:15240–15248. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Saus J, Wieslander J, Langeveld JP, Quinones S and Hudson BG: Identification of the Goodpasture antigen as the alpha 3(IV) chain of collagen IV. J Biol Chem. 263:13374–13380. 1988.PubMed/NCBI

13 

Wang SJ, Liu XH, Ji YB and Chen N: Study on Biological Activity of Two Modified Anti-tumor Peptide of Tumstatin. Biochemistry and Biophysics. 34:1152–1161. 2007.(In Chinese).

14 

Gao Y, Yu Y, Lu S, Sun BC and Wang XH: Cloning and expression of human tumstatin gene in E. Coli. Chinese Journal of Biochemical Pharmaceutics. 26:324–327. 2005.(In Chinese).

15 

Li Y, Liu XH, Lin XS, et al: Inhibitory effect of tumstatin related peptide T42 on human umbilical vein endothelial cells and human gastric adenocarcinoma. J Clin Rehab Tissue Engineer Res. 11:1837–1840. 2007.(In Chinese).

16 

Su XJ, Lin XS, Wu FJ and Yuan LJ: Antitumor activity of tumor chalone 42 peptide combined with cyclophosphamide. China Cancer. 5:334–337. 2010.

17 

Jones MS II, Harrach B, Ganac RD, et al: New adenovirus species found in a patient presenting with gastroenteritis. J Virol. 81:5978–5984. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Bernt KM, Ni S, Tieu AT and Lieber A: Assessment of a combined, adenovirus-mediated oncolytic and immunostimulatory tumor therapy. Cancer Res. 65:4343–4352. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Takehashi M, Kanatsu-Shinohara M, Inoue K, et al: Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. Proc Natl Acad Sci USA. 104:2596–2601. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Qi DD, Chen YL, Guo JZ, Zhu GM, Zhang T, Jiang S and Wang YZ: Constructiong of recombinant adenovirus vector with 4T42 peptide gene, and study of the effect of anti- SKBR3 cell in vitro. Chinese Journal of Gerontology. 32:2781–2783. 2012.(In Chinese).

21 

Maeshima Y, Yerramalla UL, Dhanabal M, et al: Extracellular matrix-derived peptide binds to alpha(v)beta(3) integrin and inhibits angiogenesis. J Biol Chem. 276:31959–31968. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Kawaguchi T, Yamashita Y, Kanamori M, et al: The PTEN/Akt pathway dictates the direct alphaVbeta3-dependent growth-inhibitory action of an active fragment of tumstatin in glioma cells in vitro and in vivo. Cancer Res. 66:11331–11340. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Maeshima Y, Sudhakar A, Lively JC, et al: Tumstatin, an endothelial cell-specific inhibitor of protein synthesis. Science. 295:140–143. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Zhang GM, Zhang YM, Fu SB, et al: Effects of cloned tumstatin-related and angiogenesis-inhibitory peptides on proliferation and apoptosis of endothelial cells. Chin Med J (Engl). 121:2324–2330. 2008.

25 

Shahan TA, Ziaie Z, Pasco S, et al: Identification of CD47/integrin-associated protein and alpha(v)beta3 as two receptors for the alpha3(IV) chain of type IV collagen on tumor cells. Cancer Res. 59:4584–4590. 1999.PubMed/NCBI

26 

Moreno-Manzano V, Lucio-Cazana J, Konta T, Nakayama K and Kitamura M: Enhancement of TNF-alpha-induced apoptosis by immobilized arginine-glycine-aspartate: involvement of a tyrosine kinase-dependent, MAP kinase-independent mechanism. Biochem Biophys Res Commun. 277:293–298. 2000. View Article : Google Scholar : PubMed/NCBI

27 

Spector DJ, Halbert DN and Raskas HJ: Regulation of integrated adenovirus sequences during adenovirus infection of transformed cells. J Virol. 36:860–871. 1980.PubMed/NCBI

28 

Graham FL, Smiley J, Russell WC and Nairn R: Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 36:59–74. 1977. View Article : Google Scholar : PubMed/NCBI

29 

Silman NJ and Fooks AR: Biophysical targeting of adenovirus vectors for gene therapy. Curr Opin Mol Ther. 2:524–531. 2000.

30 

He TC, Zhou S, da Costa LT, Yu J, Kinzler KW and Vogelstein B: A simplified system for generating recombinant adenoviruses. Pro Natl Acad Sci USA. 95:2509–2514. 1998. View Article : Google Scholar

31 

Li YJ, Sun LC, He Y, et al: The anti-tumor properties of two tumstatin peptide fragments in human gastric carcinoma. Acta Pharmacol Sin. 30:1307–1315. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Qi DD, Zhang TT, Chen QX, Wang GZ, Sui GY, Hao XW, Sun S, Song X, Chen YL, Chen YL, et al: Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis. Mol Med Rep 11: 2083-2091, 2015.
APA
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G. ... Chen, Y. (2015). Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis. Molecular Medicine Reports, 11, 2083-2091. https://doi.org/10.3892/mmr.2014.2910
MLA
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G., Hao, X., Sun, S., Song, X., Chen, Y."Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis". Molecular Medicine Reports 11.3 (2015): 2083-2091.
Chicago
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G., Hao, X., Sun, S., Song, X., Chen, Y."Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis". Molecular Medicine Reports 11, no. 3 (2015): 2083-2091. https://doi.org/10.3892/mmr.2014.2910
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Qi DD, Zhang TT, Chen QX, Wang GZ, Sui GY, Hao XW, Sun S, Song X, Chen YL, Chen YL, et al: Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis. Mol Med Rep 11: 2083-2091, 2015.
APA
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G. ... Chen, Y. (2015). Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis. Molecular Medicine Reports, 11, 2083-2091. https://doi.org/10.3892/mmr.2014.2910
MLA
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G., Hao, X., Sun, S., Song, X., Chen, Y."Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis". Molecular Medicine Reports 11.3 (2015): 2083-2091.
Chicago
Zhang, X., Qi, D., Zhang, T., Chen, Q., Wang, G., Sui, G., Hao, X., Sun, S., Song, X., Chen, Y."Antitumor activity of adenoviral vector containing T42 and 4xT42 peptide gene through inducing apoptosis of tumor cells and suppressing angiogenesis". Molecular Medicine Reports 11, no. 3 (2015): 2083-2091. https://doi.org/10.3892/mmr.2014.2910
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team