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Article Open Access

Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells

Corrigendum in: /mmr/12/6/8329
  • Authors:
    • Du Meng
    • Rui Liu
    • Li Pei
    • Lei Hou
    • Qian Ning
    • Qing Yu
    • Lu Feng
    • Xinhan Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Meng et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 2555-2561
    |
    Published online on: December 3, 2014
       https://doi.org/10.3892/mmr.2014.3043
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Abstract

The aim of the present study was to investigate the feasibility of lentiviral‑mediated Cys‑Asn‑Gly‑Arg‑Cys (CNGRC) peptide gene transduction in adipose stem cells. Adipose stem cells were prepared using enzymatic digestion and repeated adherence methods and identified in culture by immunofluorescence staining of surface markers. The pluripotency of the cultured adipose stem cells was confirmed by their induced differentiation into bone and fat cells. Following polymerase chain reaction amplification, the gene sequence for the CNGRC peptide was cloned into a lentiviral vector, which was then co‑transfected into 293T cells with packaging plasmids Helper 1.0 and Helper 2.0. The lentiviruses carrying the CNGRC peptide gene were then harvested and used to transfect adipose stem cells. Following transduction, expression of CNGRC in adipose stem cells was detected using western blot analysis. Adipose stem cells in culture were successfully induced to differentiate into adipocytes and osteoblasts and the lentiviral vector containing CNGRC‑3Flag‑EGFP was successfully constructed. Following transduction, western blot analysis and immunofluorescence staining demonstrated expression of the CNGRC protein in adipose stem cells. This suggested that adipose stem cell lines expressing the CNGRC peptide were successfully established.
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Copy and paste a formatted citation
Spandidos Publications style
Meng D, Liu R, Pei L, Hou L, Ning Q, Yu Q, Feng L and Zhao X: Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329. Mol Med Rep 11: 2555-2561, 2015.
APA
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q. ... Zhao, X. (2015). Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329. Molecular Medicine Reports, 11, 2555-2561. https://doi.org/10.3892/mmr.2014.3043
MLA
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q., Feng, L., Zhao, X."Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329". Molecular Medicine Reports 11.4 (2015): 2555-2561.
Chicago
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q., Feng, L., Zhao, X."Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329". Molecular Medicine Reports 11, no. 4 (2015): 2555-2561. https://doi.org/10.3892/mmr.2014.3043
Copy and paste a formatted citation
x
Spandidos Publications style
Meng D, Liu R, Pei L, Hou L, Ning Q, Yu Q, Feng L and Zhao X: Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329. Mol Med Rep 11: 2555-2561, 2015.
APA
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q. ... Zhao, X. (2015). Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329. Molecular Medicine Reports, 11, 2555-2561. https://doi.org/10.3892/mmr.2014.3043
MLA
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q., Feng, L., Zhao, X."Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329". Molecular Medicine Reports 11.4 (2015): 2555-2561.
Chicago
Meng, D., Liu, R., Pei, L., Hou, L., Ning, Q., Yu, Q., Feng, L., Zhao, X."Lentivirus vector-mediated gene transduction of CNGRC peptide in rat adipose stem cells Corrigendum in /mmr/12/6/8329". Molecular Medicine Reports 11, no. 4 (2015): 2555-2561. https://doi.org/10.3892/mmr.2014.3043
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