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Article

A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography

  • Authors:
    • Hang Sik Roh
    • Hye Min Song
    • Bo Reum Yun
    • Hyun Kyung Kang
    • Keum Suk Choi
    • Yun Ju Park
    • Dong Sub Kim
    • Seung Hee Kim
    • In Pil Mo
    • Beum‑Soo An
    • Chi Young Ahn
  • View Affiliations / Copyright

    Affiliations: Biologics Research Division, National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Cheongwon‑gun, Chungcheongbuk‑do 363‑951, Republic of Korea, College of Veterinary Medicine, Chungbuk National University, Cheongju‑si, Chungcheongbuk‑do 361‑763, Republic of Korea, Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, Miryang, Gyeongsangnam‑do 627‑706, Republic of Korea
  • Pages: 2819-2824
    |
    Published online on: December 4, 2014
       https://doi.org/10.3892/mmr.2014.3049
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Abstract

Single radial immunodiffusion (SRID) assay requires a reference antigen and an antibody to the hemagglutinin (HA) of an influenza vaccine. As it takes 2‑3 months to develop the reference antigen, vaccine development is delayed in cases of an influenza pandemic. In the present study, the measurement of the HA content of influenza vaccines was assessed using size exclusion high performance liquid chromatography (SE‑HPLC) for the rapid development of a pandemic vaccine. When the 2009 H1N1 reference antigen, pandemic 2009 H1N1 vaccine and 2010 seasonal influenza vaccines were analyzed by SE‑HPLC, the HA of the reference antigen and vaccines was specifically separated. The presence and specificity of HA were evidenced with immunoprecipitation and ELISA assays. For the influenza vaccines, the chromatogram pattern and retention time of HA were similar among the antigen types (2009 H1N1, 2010 H3N2 and 2010 B). In addition, when SE‑HPLC was applied, the ratio of HA chromatogram to peak area revealed a significant correlation with HA concentration for the reference antigen and vaccine. The result of the HA content calculation based on SE‑HPLC exhibited 99.91‑100% similarity, compared with that of SRID. These findings suggest that the measurement of peak area ratio/HA content using SE‑HPLC may be a substitute for SRID and rapidly measure HA content to enable faster development of a vaccine during an influenza pandemic.
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1 

Schild GC, Wood JM and Newman RW: A single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen. Proposals for an assay method for the haemagglutinin content of influenza vaccines. Bull World Health Organ. 52:223–231. 1975.PubMed/NCBI

2 

Wood JM, Schild GC, Newman RW and Seagroatt V: An improved single-radial-immunodiffusion technique for the assay of influenza haemagglutinin antigen: application for potency determinations of inactivated whole virus and subunit vaccines. J Biol Stand. 5:237–247. 1977. View Article : Google Scholar : PubMed/NCBI

3 

Wood JM, Mumford J, Schild GC, Webster RG and Nicholson KG: Single-radial-immunodiffusion potency tests of inactivated influenza vaccines for use in man and animals. Dev Biol Stand. 64:169–177. 1986.PubMed/NCBI

4 

Williams MS: Single-radial-immunodiffusion as an in vitro potency assay for human inactivated viral vaccines. Vet Microbiol. 37:253–262. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Hu J, Liu DC and Wang TR: Clinical application of the single radial immunodiffusion (SRID) technic to detect antitubercle bacillus antibody. Zhonghua Jie He He Hu Xi Za Zhi. 16:270–271. 318–279. 1993.(In Chinese).

6 

Gerdil C: The annual production cycle for influenza vaccine. Vaccine. 21:1776–1779. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Kapteyn JC, Saidi MD, Dijkstra R, et al: Haemagglutinin quantification and identification of influenza A&B strains propagated in PER. C6 cells: a novel RP-HPLC method. Vaccine. 24:3137–3144. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Kapteyn JC, Porre AM, de Rond EJ, et al: HPLC-based quantification of haemagglutinin in the production of egg- and MDCK cell-derived influenza virus seasonal and pandemic vaccines. Vaccine. 27:1468–1477. 2009. View Article : Google Scholar

9 

Garcia-Canas V, Lorbetskie B, Bertrand D, Cyr TD and Girard M: Selective and quantitative detection of influenza virus proteins in commercial vaccines using two-dimensional high-performance liquid chromatography and fluorescence detection. Anal Chem. 79:3164–3172. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Williams TL, Luna L, Guo Z, et al: Quantification of influenza virus hemagglutinins in complex mixtures using isotope dilution tandem mass spectrometry. Vaccine. 26:2510–2520. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Peyre M, Fusheng G, Desvaux S and Roger F: Avian influenza vaccines: a practical review in relation to their application in the field with a focus on the Asian experience. Epidemiol Infect. 137:1–21. 2009. View Article : Google Scholar

12 

Li C, Shao M, Cui X, et al: Application of deglycosylation and electrophoresis to the quantification of influenza viral hemagglutinins facilitating the production of 2009 pandemic influenza (H1N1) vaccines at multiple manufacturing sites in China. Biologicals. 38:284–289. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Wood JM and Levandowski RA: The influenza vaccine licensing process. Vaccine. 21:1786–1788. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Ferguson NM, Cummings DA, Fraser C, Cajka JC, Cooley PC and Burke DS: Strategies for mitigating an influenza pandemic. Nature. 442:448–452. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Roh HS, Song HM, Yun BR, Kang HK, Choi KS, Park YJ, Kim DS, Kim SH, Mo IP, An BS, An BS, et al: A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography. Mol Med Rep 11: 2819-2824, 2015.
APA
Roh, H.S., Song, H.M., Yun, B.R., Kang, H.K., Choi, K.S., Park, Y.J. ... Ahn, C.Y. (2015). A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography. Molecular Medicine Reports, 11, 2819-2824. https://doi.org/10.3892/mmr.2014.3049
MLA
Roh, H. S., Song, H. M., Yun, B. R., Kang, H. K., Choi, K. S., Park, Y. J., Kim, D. S., Kim, S. H., Mo, I. P., An, B., Ahn, C. Y."A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography". Molecular Medicine Reports 11.4 (2015): 2819-2824.
Chicago
Roh, H. S., Song, H. M., Yun, B. R., Kang, H. K., Choi, K. S., Park, Y. J., Kim, D. S., Kim, S. H., Mo, I. P., An, B., Ahn, C. Y."A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography". Molecular Medicine Reports 11, no. 4 (2015): 2819-2824. https://doi.org/10.3892/mmr.2014.3049
Copy and paste a formatted citation
x
Spandidos Publications style
Roh HS, Song HM, Yun BR, Kang HK, Choi KS, Park YJ, Kim DS, Kim SH, Mo IP, An BS, An BS, et al: A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography. Mol Med Rep 11: 2819-2824, 2015.
APA
Roh, H.S., Song, H.M., Yun, B.R., Kang, H.K., Choi, K.S., Park, Y.J. ... Ahn, C.Y. (2015). A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography. Molecular Medicine Reports, 11, 2819-2824. https://doi.org/10.3892/mmr.2014.3049
MLA
Roh, H. S., Song, H. M., Yun, B. R., Kang, H. K., Choi, K. S., Park, Y. J., Kim, D. S., Kim, S. H., Mo, I. P., An, B., Ahn, C. Y."A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography". Molecular Medicine Reports 11.4 (2015): 2819-2824.
Chicago
Roh, H. S., Song, H. M., Yun, B. R., Kang, H. K., Choi, K. S., Park, Y. J., Kim, D. S., Kim, S. H., Mo, I. P., An, B., Ahn, C. Y."A hemagglutinin quantification method for development of an influenza pandemic vaccine using size exclusion high performance liquid chromatography". Molecular Medicine Reports 11, no. 4 (2015): 2819-2824. https://doi.org/10.3892/mmr.2014.3049
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