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Article

Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis

  • Authors:
    • Jong Seok Lee
    • Young Rae Kim
    • Jun Myoung Park
    • Young Eon Kim
    • Nam In Baek
    • Eock Kee Hong
  • View Affiliations / Copyright

    Affiliations: Department of Bioengineering and Technology, College of Engineering, Kangwon National University, Chuncheon, Gangwon‑do 200‑701, Republic of Korea, Division of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Gyeonggi‑Do 463‑746, Republic of Korea, Department of Oriental Medicinal Materials and Processing, Kyung Hee University, Yongin, Gyeonggi‑do 446‑701, Republic of Korea
  • Pages: 2723-2728
    |
    Published online on: December 11, 2014
       https://doi.org/10.3892/mmr.2014.3078
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Abstract

The present study investigated the cytoprotective effects of cyanidin‑3‑glucoside (C3G), isolated from mulberry fruits, on the glucotoxicity‑induced apoptosis of pancreatic β‑cells to evaluate the antidiabetic effects of this compound. MIN6N pancreatic β‑cells were used to investigate the cytoprotective effects of C3G. In addition, the effects of C3G on the glucotoxicity‑induced apoptosis of pancreatic β‑cells was evaluated using MTT assay, immunofluorescent staining, flow cytometric and western blot analyses. The pancreatic β‑cells cultured under high glucose conditions exhibited distinct apoptotic features. C3G decreased the generation of intracellular reactive oxygen species, DNA fragmentation and the rate of apoptosis. C3G also prevented pancreatic β‑cell apoptosis induced by high glucose conditions by interfering with the intrinsic apoptotic pathways. In addition, C3G treatment resulted in increased insulin secretion compared with treatment with high glucose only. In conclusion, the results of the present study suggested that C3G obtained from mulberry fruits may be a potential phytotherapeutic agent for the prevention of diabetes.
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Copy and paste a formatted citation
Spandidos Publications style
Lee JS, Kim YR, Park JM, Kim YE, Baek NI and Hong EK: Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. Mol Med Rep 11: 2723-2728, 2015.
APA
Lee, J.S., Kim, Y.R., Park, J.M., Kim, Y.E., Baek, N.I., & Hong, E.K. (2015). Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. Molecular Medicine Reports, 11, 2723-2728. https://doi.org/10.3892/mmr.2014.3078
MLA
Lee, J. S., Kim, Y. R., Park, J. M., Kim, Y. E., Baek, N. I., Hong, E. K."Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis". Molecular Medicine Reports 11.4 (2015): 2723-2728.
Chicago
Lee, J. S., Kim, Y. R., Park, J. M., Kim, Y. E., Baek, N. I., Hong, E. K."Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis". Molecular Medicine Reports 11, no. 4 (2015): 2723-2728. https://doi.org/10.3892/mmr.2014.3078
Copy and paste a formatted citation
x
Spandidos Publications style
Lee JS, Kim YR, Park JM, Kim YE, Baek NI and Hong EK: Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. Mol Med Rep 11: 2723-2728, 2015.
APA
Lee, J.S., Kim, Y.R., Park, J.M., Kim, Y.E., Baek, N.I., & Hong, E.K. (2015). Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis. Molecular Medicine Reports, 11, 2723-2728. https://doi.org/10.3892/mmr.2014.3078
MLA
Lee, J. S., Kim, Y. R., Park, J. M., Kim, Y. E., Baek, N. I., Hong, E. K."Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis". Molecular Medicine Reports 11.4 (2015): 2723-2728.
Chicago
Lee, J. S., Kim, Y. R., Park, J. M., Kim, Y. E., Baek, N. I., Hong, E. K."Cyanidin-3‑glucoside isolated from mulberry fruits protects pancreatic β-cells against glucotoxicity-induced apoptosis". Molecular Medicine Reports 11, no. 4 (2015): 2723-2728. https://doi.org/10.3892/mmr.2014.3078
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