Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2015 Volume 11 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Activin A regulates proliferation, invasion and migration in osteosarcoma cells

  • Authors:
    • Jianwei Zhu
    • Fan Liu
    • Quanming Wu
    • Xiancheng Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
  • Pages: 4501-4507
    |
    Published online on: January 30, 2015
       https://doi.org/10.3892/mmr.2015.3284
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Activin A is a member of the TGF‑β superfamily. Previous studies have demonstrated that activin A exhibited pluripotent effects in several tumours. However, the roles of activin A signaling in osteosarcoma pathogenesis have not been previously investigated. Therefore, the present study aimed to investigate the effects of activin A on osteosarcoma cell proliferation, invasion and migration. Firstly, the expression of activin A in osteosarcoma cell lines (MG63, SaOS‑2 and U2OS) and a human osteoblastic cell line (hFOB1.19) was detected using reverse transcription quantitative polymerase chain reaction and western blotting. Activin A was upregulated in osteosarcoma cell lines compared with hFOB1.19 cells. To investigate the effects of activin A on osteosarcoma cell proliferation, invasion and migration, MG63 cells were generated in which activin A was either overexpressed or depleted. MTT staining, propidium iodide staining and a Transwell assay were used to analyze the cell cycle, proliferation, invasion and migration of MG63 cells, respectively. The results of the present study revealed that the abilities of proliferation, invasion and migration were suppressed in MG63 cells in which activin A was depleted, while they were enhanced in activin A-overexpressing cells. In conclusion, the results of the present study suggested that activin A may facilitate proliferation, invasion and migration of osteosarcoma cells, and it may therefore be a potential target for the treatment of osteosarcoma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Endo-Munoz L, Evdokiou A and Saunders NA: The role of osteoclasts and tumour-associated macrophages in osteosarcoma metastasis. Biochim Biophys Acta. 1826:434–442. 2012.PubMed/NCBI

2 

Poletajew S, Fus L and Wasiutyński A: Current concepts on pathogenesis and biology of metastatic osteosarcoma tumours. Ortop Traumatol Rehabil. 13:537–545. 2011.In English, Polish. View Article : Google Scholar

3 

Tanzawa H, Uchiyama S and Sato K: Statistical observation of osteosarcoma of the maxillofacial region in Japan. Analysis of 114 Japanese cases reported between 1930 and 1989. Oral Surg Oral Med Oral Pathol. 72:444–448. 1991. View Article : Google Scholar : PubMed/NCBI

4 

Lee YY, Van Tassel P, Nauert C, Raymond AK and Edeiken J: Craniofacial osteosarcomas: plain film, CT, and MR findings in 46 cases. AJR Am J Roentgenol. 150:1397–1402. 1988. View Article : Google Scholar : PubMed/NCBI

5 

Harting MT and Blakely ML: Management of osteosarcoma pulmonary metastases. Semin Pediatr Surg. 15:25–29. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Liu AN, Zhu ZH, Chang SJ and Hang XS: Twist expression associated with the epithelial-mesenchymal transition in gastric cancer. Mol Cell Biochem. 367:195–203. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Cui J, Wang W, Li Z, Zhang Z, Wu B and Zeng L: Epigenetic changes in osteosarcoma. Bull Cancer. 98:E62–E68. 2011.PubMed/NCBI

8 

Chen YG, Wang Q, Lin SL, Chang CD, Chuang J and Ying SY: Activin signaling and its role in regulation of cell proliferation, apoptosis, and carcinogenesis. Exp Biol Med (Maywood). 231:534–544. 2006.

9 

Sulyok S, Wankell M, Alzheimer C and Werner S: Activin: an important regulator of wound repair, fibrosis, and neuroprotection. Mol Cell Endocrinol. 225:127–132. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Antsiferova M and Werner S: The bright and the dark sides of activin in wound healing and cancer. J Cell Sci. 125:3929–3937. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Kypriotou M, Rivero D, Haller S, et al: Activin A inhibits antigen-induced allergy in murine epicutaneous sensitization. Front Immunol. 4:2462013. View Article : Google Scholar : PubMed/NCBI

12 

Neel JC and Lebrun JJ: Activin and TGFβ regulate expression of the microRNA-181 family to promote cell migration and invasion in breast cancer cells. Cell Signal. 25:1556–1566. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Deli A, Kreidl E, Santifaller S, et al: Activins and activin antagonists in hepatocellular carcinoma. World J Gastroenterol. 14:1699–1709. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Matsuo SE, Leoni SG, Colquhoun A and Kimura ET: Transforming growth factor-beta1 and activin A generate anti-proliferative signaling in thyroid cancer cells. J Endocrinol. 190:141–150. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Kaneda H, Arao T, Matsumoto K, et al: Activin A inhibits vascular endothelial cell growth and suppresses tumour angiogenesis in gastric cancer. Br J Cancer. 105:1210–1217. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Burdette JE, Jeruss JS, Kurley SJ, Lee EJ and Woodruff TK: Activin A mediates growth inhibition and cell cycle arrest through Smads in human breast cancer cells. Cancer Res. 65:7968–7975. 2005.PubMed/NCBI

17 

Ramachandran A, Marshall ES, Love DR, Baguley BC and Shelling AN: Activin is a potent growth suppressor of epithelial ovarian cancer cells. Cancer Lett. 285:157–165. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Ottley E and Gold E: Insensitivity to the growth inhibitory effects of activin A: an acquired capability in prostate cancer progression. Cytokine Growth Factor Rev. 23:119–125. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chang KP, Kao HK, Liang Y, et al: Overexpression of activin A in oral squamous cell carcinoma: association with poor prognosis and tumour progression. Ann Surg Oncol. 17:1945–1956. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Hoda MA, Munzker J, Ghanim B, et al: Suppression of activin A signals inhibits growth of malignant pleural mesothelioma cells. Br J Cancer. 107:1978–1986. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Marino FE, Risbridger G and Gold E: The therapeutic potential of blocking the activin signalling pathway. Cytokine Growth Factor Rev. 24:477–484. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Yoshinaga K, Mimori K, Inoue H, et al: Activin A enhances MMP-7 activity via the transcription factor AP-1 in an esophageal squamous cell carcinoma cell line. Int J Oncol. 33:453–459. 2008.PubMed/NCBI

23 

Antsiferova M, Huber M, Meyer M, et al: Activin enhances skin tumourigenesis and malignant progression by inducing a pro-tumourigenic immune cell response. Nat Commun. 2:5762011. View Article : Google Scholar : PubMed/NCBI

24 

Terpos E, Kastritis E, Christoulas D, et al: Circulating activin A is elevated in patients with advanced multiple myeloma and correlates with extensive bone involvement and inferior survival; no alterations post-lenalidomide and dexamethasone therapy. Ann Oncol. 23:2681–2686. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

26 

Wang HJ, Ruan HJ, He XJ, et al: MicroRNA-101 is down-regulated in gastric cancer and involved in cell migration and invasion. Eur J Cancer. 46:2295–2303. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Phillips DJ, Jones KL, Clarke IJ, Scheerlinck JP and de Kretser DM: Activin A: from sometime reproductive factor to genuine cytokine. Vet Immunol Immunopathol. 108:23–27. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Sobral LM, Bufalino A, Lopes MA, Graner E, Salo T and Coletta RD: Myofibroblasts in the stroma of oral cancer promote tumorigenesis via secretion of activin A. Oral Oncol. 47:840–846. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Kang HY, Huang HY, Hsieh CY, et al: Activin A enhances prostate cancer cell migration through activation of androgen receptor and is overexpressed in metastatic prostate cancer. J Bone Miner Res. 24:1180–1193. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Le Bras GF, Allison GL, Richards NF, Ansari SS, Washington MK and Andl CD: CD44 upregulation in E-cadherin-negative esophageal cancers results in cell invasion. PloS one. 6:e270632011. View Article : Google Scholar : PubMed/NCBI

31 

Incorvaia L, Badalamenti G, Rini G, et al: MMP-2, MMP-9 and activin A blood levels in patients with breast cancer or prostate cancer metastatic to the bone. Anticancer Res. 27:1519–1525. 2007.PubMed/NCBI

32 

Jianwei Z, Fan L, Xiancheng L, Enzhong B, Shuai L and Can L: MicroRNA 181a improves proliferation and invasion, suppresses apoptosis of osteosarcoma cell. Tumour Biol. 34:3331–3337. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Liu F, Wu Q and Liu X: Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Mol Med Rep 11: 4501-4507, 2015.
APA
Zhu, J., Liu, F., Wu, Q., & Liu, X. (2015). Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Molecular Medicine Reports, 11, 4501-4507. https://doi.org/10.3892/mmr.2015.3284
MLA
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11.6 (2015): 4501-4507.
Chicago
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11, no. 6 (2015): 4501-4507. https://doi.org/10.3892/mmr.2015.3284
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Liu F, Wu Q and Liu X: Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Mol Med Rep 11: 4501-4507, 2015.
APA
Zhu, J., Liu, F., Wu, Q., & Liu, X. (2015). Activin A regulates proliferation, invasion and migration in osteosarcoma cells. Molecular Medicine Reports, 11, 4501-4507. https://doi.org/10.3892/mmr.2015.3284
MLA
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11.6 (2015): 4501-4507.
Chicago
Zhu, J., Liu, F., Wu, Q., Liu, X."Activin A regulates proliferation, invasion and migration in osteosarcoma cells". Molecular Medicine Reports 11, no. 6 (2015): 4501-4507. https://doi.org/10.3892/mmr.2015.3284
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team