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Article

Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes

  • Authors:
    • Hong Jiang
    • Zheng Li
    • Xiaohua Li
    • Jianguo Xia
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Food Science and Human Nutrition, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA
  • Pages: 4657-4663
    |
    Published online on: February 6, 2015
       https://doi.org/10.3892/mmr.2015.3312
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Abstract

Exosomes are small membrane vesicles of endocytic origin. They are derived from various cells, including tumor cells, and may serve as important modulators of intercellular communication. The present study established a U‑87 MG human glioblastoma cell line that showed a stable expression of green fluorescent protein (GFP; U‑87‑GFP). Two types of human tumor cell lines, U‑87‑GFP and LoVo human colon cancer cells, were demonstrated to communicate through the transfer of GFP messenger (m)RNA by exosomes. Furthermore, GFP mRNAs delivered by the U‑87‑GFP cell‑derived exosomes were translated into functional proteins in the recipient LoVo cells. In addition, LoVo cells were demonstrated to uptake exosomes derived from the U‑87‑GFP cells by clathrin‑mediated endocytosis. These results indicate that tumor cell‑derived exosomes may represent vesicular carriers that regulate gene expression, which may provide a pathway of intercellular communication in the tumor microenvironment during multiorgan tumorigenesis. The exosome uptake pathway may have potential therapeutic applications in multiorgan tumorigenesis.
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1 

Zitvogel L, Regnault A, Lozier A, et al: Eradication of established murine tumors using a novel cell-free vaccine: dendritic cell derived exosomes. Nat Med. 4:594–600. 1998. View Article : Google Scholar : PubMed/NCBI

2 

Théry C, Zitvogel L and Amigorena S: Exosomes: composition, biogenesis and function. Nat Rev Immunol. 2:569–579. 2002.PubMed/NCBI

3 

Johnstone RM, Bianchini A and Teng K: Reticulocyte maturation and exosome release: transferrin receptor containing exosomes shows multiple plasma membrane functions. Blood. 74:1844–1851. 1989.PubMed/NCBI

4 

Théry C, Boussac M, Véron P, et al: Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. J Immunol. 166:7309–7318. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Clayton A, Turkes A, Dewitt S, Steadman R, Mason MD and Hallett MB: Adhesion and signaling by B cell-derived exosomes: the role of integrins. FASEB J. 18:977–979. 2004.PubMed/NCBI

6 

Blanchard N, Lankar D, Faure F, et al: TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex. J Immunol. 168:3235–3241. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ and Lötvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Van Niel G, Mallegol J, Bevilacqua C, et al: Intestinal epithelial exosomes carry MHC class II/peptides able to inform the immune system in mice. Gut. 52:1690–1697. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Wolfers J, Lozier A, Raposo G, et al: Tumor-derived exosomes are a source of shared tumor rejection antigens for CTL cross-priming. Nat Med. 7:297–303. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Nilsson J, Skog J, Nordstrand A, et al: Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer. Br J Cancer. 100:1603–1607. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G and Bonnerot C: Exosomal-like vesicles are present in human blood plasma. Int Immunol. 17:879–887. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Admyre C, Johansson SM, Qazi KR, et al: Exosomes with immune modulatory features are present in human breast milk. J Immunol. 179:1969–1978. 2007. View Article : Google Scholar : PubMed/NCBI

13 

André F, Chaput N, Schartz NE, et al: Exosomes as potent cell-free peptide-based vaccine. I. Dendritic cell-derived exosomes transfer functional MHC class I/peptide complexes to dendritic cells. J Immunol. 172:2126–2136. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Karlsson M, Lundin S, Dahlgren U, Kahu H, Pettersson I and Telemo E: ‘Tolerosomes’ are produced by intestinal epithelial cells. Eur J Immunol. 31:2892–2900. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Stoner G, Casto B, Ralston S, Roebuck B, Pereira C and Bailey G: Development of a multi-organ rat model for evaluating chemopreventive agents: efficacy of indole-3-carbinol. Carcinogenesis. 23:265–272. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Pazzaglia S: Ptc1 heterozygous knockout mice as a model of multi-organ tumorigenesis. Cancer Lett. 234:124–134. 2006. View Article : Google Scholar

17 

Yamagishi M, Natsume M, Osakabe N, et al: Chemoprevention of lung carcinogenesis by cacao liquor proanthocyanidins in a male rat multi-organ carcinogenesis model. Cancer Lett. 191:49–57. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Skog J, Würdinger T, van Rijn S, et al: Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol. 10:1470–1476. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Fero ML, Rivkin M, Tasch M, et al: A Syndrome of multiorgan hyperplasia with features of gigantism, tumorigenesis, and female sterility in p27(Kip1)-deficient mice. Cell. 85:733–744. 1996. View Article : Google Scholar : PubMed/NCBI

20 

Al-Nedawi K, Meehan B, Micallef J, et al: Intercellular transfer of the oncogenic receptor EGFRvIII by microvesicles derived from tumour cells. Nat Cell Biol. 10:619–624. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Baj-Krzyworzeka M, Szatanek R, Weglarczyk K, et al: Tumour-derived microvesicles carry several surface determinants and mRNA of tumour cells and transfer some of these determinants to monocytes. Cancer Immunol Immunother. 55:808–818. 2006. View Article : Google Scholar

22 

Martinez MC, Larbret F, Zobairi F, et al: Transfer of differentiation signal by membrane microvesicles harboring hedgehog morphogens. Blood. 108:3012–3020. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Gratton SEA, Ropp PA, Pohlhaus PD, et al: The effect of particle design on cellular internalization pathways. Proc Natl Acad Sci USA. 105:11613–11618. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Weggen S, Eriksen JL, Das P, et al: A subset of NSAIDs lower amyloidogenic Abeta42 independently of cyclooxygenase activity. Nature. 414:212–216. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Ratajczak J, Wysoczynski M, Hayek F, Janowska-Wieczorek A and Ratajczak MZ: Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication. Leukemia. 20:1487–1495. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Yuan A, Farber EL, Rapoport AL, et al: Transfer of microRNAs by embryonic stem cell microvesicles. PLoS One. 4:e47222009. View Article : Google Scholar : PubMed/NCBI

27 

Simons M and Raposo G: Exosomes - vesicular carriers for intercellular communication. Curr Opin Cell Biol. 21:575–581. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Janowska-Wieczorek A, Wysoczynski M, Kijowski J, et al: Microvesicles derived from activated platelets induce metastasis and angiogenesis in lung cancer. Int J Cancer. 113:752–760. 2005. View Article : Google Scholar

29 

Liu C, Yu S, Zinn K, et al: Murine mammary carcinoma exosomes promote tumor growth by suppression of NK cell function. J Immunol. 176:1375–1385. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Schorey JS and Bhatnagar S: Exosome function: from tumor immunology to pathogen biology. Traffic. 9:871–881. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Ratajczak J, Miekus K, Kucia M, et al: Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. Leukemia. 20:847–856. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Hunter MP, Ismail N, Zhang X, et al: Detection of microRNA expression in human peripheral blood microvesicles. PLoS One. 3:e36942008. View Article : Google Scholar : PubMed/NCBI

33 

Taylor DD and Gercel-Taylor C: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol. 110:13–21. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jiang H, Li Z, Li X and Xia J: Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes. Mol Med Rep 11: 4657-4663, 2015.
APA
Jiang, H., Li, Z., Li, X., & Xia, J. (2015). Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes. Molecular Medicine Reports, 11, 4657-4663. https://doi.org/10.3892/mmr.2015.3312
MLA
Jiang, H., Li, Z., Li, X., Xia, J."Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes". Molecular Medicine Reports 11.6 (2015): 4657-4663.
Chicago
Jiang, H., Li, Z., Li, X., Xia, J."Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes". Molecular Medicine Reports 11, no. 6 (2015): 4657-4663. https://doi.org/10.3892/mmr.2015.3312
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang H, Li Z, Li X and Xia J: Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes. Mol Med Rep 11: 4657-4663, 2015.
APA
Jiang, H., Li, Z., Li, X., & Xia, J. (2015). Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes. Molecular Medicine Reports, 11, 4657-4663. https://doi.org/10.3892/mmr.2015.3312
MLA
Jiang, H., Li, Z., Li, X., Xia, J."Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes". Molecular Medicine Reports 11.6 (2015): 4657-4663.
Chicago
Jiang, H., Li, Z., Li, X., Xia, J."Intercellular transfer of messenger RNAs in multiorgan tumorigenesis by tumor cell-derived exosomes". Molecular Medicine Reports 11, no. 6 (2015): 4657-4663. https://doi.org/10.3892/mmr.2015.3312
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