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Article Open Access

Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression

  • Authors:
    • Lei Kuang
    • Guohua Lv
    • Bing Wang
    • Lei Li
    • Yuliang Dai
    • Yawei Li
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
    Copyright: © Kuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 93-98
    |
    Published online on: February 12, 2015
       https://doi.org/10.3892/mmr.2015.3341
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Abstract

Chordoma is a rare, slow‑growing primary malignant neoplasm of the axial skeleton, which arises from the remnants of the notochord. Emerging evidence suggests that microRNAs (miRs) are dysregulated in chordoma tissues and crucially involved in chordoma pathogenesis. In the present study, the expression of 11 candidate miRs were analyzed in chordoma tissues and miR‑10a and miR‑125a were found to be significantly downregulated compared with controls. Notably, the expression of the primary transcripts, pri‑miR‑125a and pri‑miR‑10a was unaltered, suggesting that disturbed microRNA expression may be induced by altered pri‑miRNA processing. Previous studies have indicated that disturbed adenosine deaminase acting on RNA (ADAR) expression is able to alter mRNA and miRNA adenosine to inosine (A‑to‑I) levels associated with cancer pathogenesis. Therefore, the expression of ADAR1 and ADAR2 was analyzed in chordoma tissues. It was found that ADAR1 was significantly overexpressed, which was accompanied by enhanced pre‑miR‑10a and pri‑miR‑125a A‑to‑I editing. These findings suggest that ADAR2 overexpression causes enhanced pre‑miR‑10a and pri‑miR‑125a A‑to‑I editing, which alters mature miR‑10a and miR‑125a expression and may contribute to chordoma pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Kuang L, Lv G, Wang B, Li L, Dai Y and Li Y: Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression. Mol Med Rep 12: 93-98, 2015.
APA
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., & Li, Y. (2015). Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression. Molecular Medicine Reports, 12, 93-98. https://doi.org/10.3892/mmr.2015.3341
MLA
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., Li, Y."Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression". Molecular Medicine Reports 12.1 (2015): 93-98.
Chicago
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., Li, Y."Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression". Molecular Medicine Reports 12, no. 1 (2015): 93-98. https://doi.org/10.3892/mmr.2015.3341
Copy and paste a formatted citation
x
Spandidos Publications style
Kuang L, Lv G, Wang B, Li L, Dai Y and Li Y: Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression. Mol Med Rep 12: 93-98, 2015.
APA
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., & Li, Y. (2015). Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression. Molecular Medicine Reports, 12, 93-98. https://doi.org/10.3892/mmr.2015.3341
MLA
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., Li, Y."Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression". Molecular Medicine Reports 12.1 (2015): 93-98.
Chicago
Kuang, L., Lv, G., Wang, B., Li, L., Dai, Y., Li, Y."Overexpression of adenosine deaminase acting on RNA 1 in chordoma tissues is associated with chordoma pathogenesis by reducing miR‑125a and miR‑10a expression". Molecular Medicine Reports 12, no. 1 (2015): 93-98. https://doi.org/10.3892/mmr.2015.3341
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