Open Access

Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer

  • Authors:
    • Xiao‑Lu Zhu
    • Shang‑Yun Wen
    • Zhi‑Hong Ai
    • Juan Wang
    • Yan‑Li Xu
    • Yin‑Cheng Teng
  • View Affiliations

  • Published online on: February 17, 2015     https://doi.org/10.3892/mmr.2015.3363
  • Pages: 55-62
  • Copyright: © Zhu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The aim of the present study was to investigate the characteristic microRNAs (miRNAs) expressed during the pre‑invasive and invasive stages of cervical cancer. A gene expression profile (GSE7803) containing 21 invasive squamous cell cervical carcinoma samples, 10 normal squamous cervical epithelium samples and seven high‑grade squamous intraepithelial cervical lesion samples, was obtained from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified using significance analysis of microarray software, and a Gene Ontology (GO) enrichment analysis was conducted using the Database for Annotation, Visualization and Integrated Discovery. The miRNAs that interacted with the identified DEGs were selected, based on the TarBase v5.0 database. Regulatory networks were constructed from these selected miRNAs along with their corresponding target genes among the DEGs. The regulatory networks were visualized using Cytoscape. A total of 1,160 and 756 DEGs were identified in the pre‑invasive and invasive stages of cervical cancer, respectively. The results of the GO enrichment demonstrated that the DEGs were predominantly involved in the immune response and the cell cycle, in the pre‑invasive and invasive stages, respectively. Furthermore, a total of 18 and 26 characteristic miRNAs were screened in the pre‑invasive and invasive stages, respectively. These miRNAs may be potential biomarkers and targets for the diagnosis and treatment of the different stages of cervical cancer.
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July-2015
Volume 12 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhu XL, Wen SY, Ai ZH, Wang J, Xu YL and Teng YC: Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer. Mol Med Rep 12: 55-62, 2015
APA
Zhu, X., Wen, S., Ai, Z., Wang, J., Xu, Y., & Teng, Y. (2015). Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer. Molecular Medicine Reports, 12, 55-62. https://doi.org/10.3892/mmr.2015.3363
MLA
Zhu, X., Wen, S., Ai, Z., Wang, J., Xu, Y., Teng, Y."Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer". Molecular Medicine Reports 12.1 (2015): 55-62.
Chicago
Zhu, X., Wen, S., Ai, Z., Wang, J., Xu, Y., Teng, Y."Screening for characteristic microRNAs between pre-invasive and invasive stages of cervical cancer". Molecular Medicine Reports 12, no. 1 (2015): 55-62. https://doi.org/10.3892/mmr.2015.3363