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Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry

  • Authors:
    • Lucas Delmonico
    • Vivian Rabello Areias
    • Rodrigo César Pinto
    • Cintia Da Silva Matos
    • Marco Felipe Franco Rosa
    • Carolina Maria De Azevedo
    • Gilda Alves
  • View Affiliations / Copyright

    Affiliations: Applied Genetics Laboratory, Haematology Service, National Cancer Institute, Rio de Janeiro, 20230-130, Brazil, Radiology Service, Gaffrée e Guinle University Hospital, Rio de Janeiro, 20270-004, Brazil
    Copyright: © Delmonico et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 159-164
    |
    Published online on: March 5, 2015
       https://doi.org/10.3892/mmr.2015.3432
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Abstract

Nipple aspirate fluid (NAF) requires investigation as a potential source of biomarkers for early diagnosis or risk assessment in breast cancer and other breast disorders. The present study demonstrated that proteins were easily extracted from dried NAF spots on Guthrie cards and were suitable for mass spectrometry analysis. NAF was obtained from 80 women, collected on Guthrie cards, between 2007 and 2010. The NAF‑proteins were extracted from the card by incubating the card in water. These proteins were then quantified and separated using one‑dimensional, 12% SDS‑PAGE, gel electrophoresis and on high‑resolution gradient gels at different concentrations (4‑12, 8‑16 and 4‑20%). The bands with the most abundant proteins were excised from the gradient gels and the proteins were identified by liquid chromatography quadrupole time of flight. Immunoglobulins, Zn‑α2‑glicoprotein, apoliprotein D and prolactin inducible protein were among those identified. The NAF-Guthrie card collection method has not been applied previously, however, NAF proteins have been identified using other collecting techniques, confirming the feasibility of the NAF Guthrie card collection method for analyzing the proteins within NAF. The NAF‑Guthrie card collecting method has multiple advantages, including being inexpensive, non‑invasive, reliable and painless, and the cards can be stored at room temperature. Examining NAF may assist in identifying individuals at a higher risk of breast cancer and in improving patient prognosis.
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Copy and paste a formatted citation
Spandidos Publications style
Delmonico L, Areias VR, Pinto RC, Matos CD, Rosa MF, De Azevedo CM and Alves G: Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry. Mol Med Rep 12: 159-164, 2015.
APA
Delmonico, L., Areias, V.R., Pinto, R.C., Matos, C.D., Rosa, M.F., De Azevedo, C.M., & Alves, G. (2015). Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry. Molecular Medicine Reports, 12, 159-164. https://doi.org/10.3892/mmr.2015.3432
MLA
Delmonico, L., Areias, V. R., Pinto, R. C., Matos, C. D., Rosa, M. F., De Azevedo, C. M., Alves, G."Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry". Molecular Medicine Reports 12.1 (2015): 159-164.
Chicago
Delmonico, L., Areias, V. R., Pinto, R. C., Matos, C. D., Rosa, M. F., De Azevedo, C. M., Alves, G."Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry". Molecular Medicine Reports 12, no. 1 (2015): 159-164. https://doi.org/10.3892/mmr.2015.3432
Copy and paste a formatted citation
x
Spandidos Publications style
Delmonico L, Areias VR, Pinto RC, Matos CD, Rosa MF, De Azevedo CM and Alves G: Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry. Mol Med Rep 12: 159-164, 2015.
APA
Delmonico, L., Areias, V.R., Pinto, R.C., Matos, C.D., Rosa, M.F., De Azevedo, C.M., & Alves, G. (2015). Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry. Molecular Medicine Reports, 12, 159-164. https://doi.org/10.3892/mmr.2015.3432
MLA
Delmonico, L., Areias, V. R., Pinto, R. C., Matos, C. D., Rosa, M. F., De Azevedo, C. M., Alves, G."Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry". Molecular Medicine Reports 12.1 (2015): 159-164.
Chicago
Delmonico, L., Areias, V. R., Pinto, R. C., Matos, C. D., Rosa, M. F., De Azevedo, C. M., Alves, G."Protein identification from dried nipple aspirate fluid on Guthrie cards using mass spectrometry". Molecular Medicine Reports 12, no. 1 (2015): 159-164. https://doi.org/10.3892/mmr.2015.3432
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