Interleukin-1β induced nuclear factor-κB binds to a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 9 promoter in human chondrosarcoma cells

Altuntas,Aynur; Halacli,Sevil  Oskay; Cakmak,Ozlem; Erden,Gonul; Akyol,Sumeyya; Ugurcu,Veli; Hirohata,Satoshi; Demircan,Kadir

doi:10.3892/mmr.2015.3444

July-2015 Volume 12 Issue 1

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

July-2015 Volume 12 Issue 1

Full Size Image

Article

Interleukin-1β induced nuclear factor-κB binds to a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 9 promoter in human chondrosarcoma cells

Authors:
- Aynur Altuntas
- Sevil Oskay Halacli
- Ozlem Cakmak
- Gonul Erden
- Sumeyya Akyol
- Veli Ugurcu
- Satoshi Hirohata
- Kadir Demircan
View Affiliations / Copyright

Affiliations: Division of Chemistry, Ankara Regional Office of Council of Forensic Medicine, Ankara 06100, Turkey, Pediatric Immunology Unit, Institute of Children's Health, Hacettepe University, Ankara 06100, Turkey, Faculty of Education, Division of Biology Education, Gazi University, Ankara 06500, Turkey, Department of Clinical Biochemistry, Medical Faculty, Hacettepe University, Ankara 06100, Turkey, Department of Medical Biology, Turgut Ozal University School of Medicine, Ankara 06105, Turkey, Department of Medical Biochemistry, Dumlupinar University Medical Faculty, Kutahya 43100, Turkey, Okayama University Graduate School of Medicine, Dentistry and Pharmacological Sciences, Okayama 700‑8558, Japan
Pages: 595-600
|
Published online on: March 6, 2015

https://doi.org/10.3892/mmr.2015.3444
Expand metrics +

Abstract

Nuclear factor‑κB (NF‑κB) is involved in the regulation of inflammation‑associated genes. NF‑κB forms dimers which bind with sequences referred to as NF‑κB sites (9‑11 bp). A disintegrin‑like and metalloproteinase with thrombospondin type 1 motif 9 (ADAMTS9) is a type of proteoglycanase, which proteolytically cleaves versican and aggrecan. ADAMTS9 is a cytokine‑inducible gene that contains binding sites for NF‑κB within its promoter region. Interleukin‑1β (IL‑1β) affects cartilage metabolism and is involved in the NF‑κB pathway. It is therefore hypothesized that NF‑κB binding with ADAMTS9 promoters may activate IL‑1β, thereby promoting chondrocytic cell growth. In the present study, the OUMS‑27 chondrocytic human chondrosarcoma cell line was treated with IL‑1β with or without inhibitors of NF‑κB signaling pathways. Chromatin immunoprecipitation (ChIP) and electromobility shift assays (EMSA) were conducted order to analyze the binding of NF‑κB with the ADAMTS9 promoter region. NF‑κB‑p65 subunit phosphorylation was promoted in IL‑1β‑treated cells, which were not treated with inhibitors of NF‑κB signaling pathways. By contrast, NF‑κB‑p65 subunit phosphorylation was inhibited in cells that had been treated with BAY‑117085, an NF‑κB pathway inhibitor. ChIP and EMSA assays demonstrated that, following treatment with IL‑1β, NF‑κB‑p65 bound to elements located at ‑1177 and ‑1335 in the ADAMTS9 promoter region, in contrast to the untreated samples. The results of the present study suggested that NF‑κB may be involved in IL‑1β‑induced activation of ADAMTS9 in human chondrocytes.

View Figures

View References

1	Tortorella MD, Burn TC, Pratta MA, et al: Purification and cloning of aggrecanase-1: a member of the ADAMTS family of proteins. Science. 284:1664–1666. 1999. View Article : Google Scholar : PubMed/NCBI
2	Apte SS: A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motifs: the ADAMTS family. Int J Biochem Cell Biol. 36:981–985. 2004. View Article : Google Scholar : PubMed/NCBI
3	Uysal S, Ünal ZN, Erdoǧan S, et al: Augmentation of ADAMTS9 gene expression by IL-1β is reversed by NF-κB and MAPK inhibitors, but not PI3 kinase inhibitors. Cell Biochem Funct. 31:539–544. 2013.
4	Demircan K, Hirohata S, Nishida K, et al: ADAMTS-9 is synergistically induced by interleukin-1beta and tumor necrosis factor alpha in OUMS-27 chondrosarcoma cells and in human chondrocytes. Arthritis Rheum. 52:1451–1460. 2005. View Article : Google Scholar : PubMed/NCBI
5	Yaykasli KO, Oohashi T, Hirohata S, Hatipoglu OF, Inagawa K, Demircan K and Ninomiya Y: ADAMTS9 activation by interleukin 1 beta via NFATc1 in OUMS-27 chondrosarcoma cells and in human chondrocytes. Mol Cell Biochem. 323:69–79. 2009. View Article : Google Scholar
6	Xu YX, Pindolia KR, Janakiraman N, Chapman RA and Gautam SC: Curcumin inhibits IL1 alpha and TNF-alpha induction of AP-1 and NF-κB DNA-binding activity in bone marrow stromal cells. Hematopathol Mol Hematol. 11:49–62. 1997.PubMed/NCBI
7	Chen D, Li X, Zhai Z and Shu HB: A novel zinc finger protein interacts with receptor-interacting protein (RIP) and inhibits tumor necrosis factor (TNF)- and IL1-induced NF-kappa B activation. J Biol Chem. 277:15985–15991. 2002. View Article : Google Scholar : PubMed/NCBI
8	Cogswell JP, Godlevski MM, Wisely GB, et al: NF-kappa B regulates IL-1 beta transcription through a consensus NF-kappa B binding site and a nonconsensus CRE-like site. J Immunol. 153:712–723. 1994.PubMed/NCBI
9	Hoesel B and Schmid JA: The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer. 12:862013. View Article : Google Scholar
10	Barnes PJ and Karin M: Nuclear factor-kappaB: a pivotal transcription factor in chronic inflammatory diseases. N Engl J Med. 336:1066–1071. 1997. View Article : Google Scholar : PubMed/NCBI
11	Hayden MS and Ghosh S: Signaling to NF-kappaB. Genes Dev. 18:2195–2224. 2004. View Article : Google Scholar : PubMed/NCBI
12	Bret C, Hose D, Reme T, et al: Gene expression profile of ADAMs and ADAMTSs metalloproteinases in normal and malignant plasma cells and in the bone marrow environment. Exp Hematol. 39:546–557. 2011. View Article : Google Scholar : PubMed/NCBI
13	Demircan K, Yonezawa T, Takigawa T, et al: ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse. Neurosci Lett. 544:25–30. 2013. View Article : Google Scholar : PubMed/NCBI
14	Lin EA and Liu CJ: The role of ADAMTSs in arthritis. Protein Cell. 1:33–47. 2010. View Article : Google Scholar
15	Kevorkian L, Young DA, Darrah C, et al: Expression profiling of metalloproteinases and their inhibitors in cartilage. Arthritis Rheum. 50:131–141. 2004. View Article : Google Scholar : PubMed/NCBI
16	Zheng X, Zhu S, Chang S, et al: Protective effects of chronic resveratrol treatment on vascular inflammatory injury in streptozotocin-induced type 2 diabetic rats: Role of NF-kappa B signaling. Eur J Pharmacol. 25:S0014–2999. 2013.
17	Choi YJ, Lee WS, Lee EG, Sung MS and Yoo WT: Sulforaphane inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of MMPs, COX-2, and PGE2. Inflammation. 37:1496–1503. 2014. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Interleukin-1β induced nuclear factor-κB binds to a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 9 promoter in human chondrosarcoma cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Related Articles